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Abstract

Two Gram-negative, cold-adapted, moderately halophilic, aerobic bacteria, designated strains M3B and M3T, were isolated from marine sediment collected from the South Shetland Islands, Antarctica. The organisms were rod-shaped, catalase- and oxidase-positive, and motile by means of polar flagella. These two psychrotolerant strains required Na and grew at NaCl concentrations of 1–15 % and temperatures between 4 and 42 °C. 16S rRNA gene sequence analysis placed strains M3B and M3T within the genus . DNA–DNA hybridization experiments between the Antarctic isolate M3B and type strains of phylogenetically related species, namely , , , , and , revealed levels of relatedness lower than 32 %. Strain M3T showed 99 % DNA relatedness to strain M3B. The DNA G+C contents of M3B and M3T were 57.1 and 57.4 mol%, respectively, and their major isoprenoid quinone was ubiquinone-9. Several phenotypic characteristics, together with data on cellular fatty acid composition, served to differentiate strains M3B and M3T from strains of related species. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains M3B and M3T belong to the same genospecies and represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M3B (=LMG 24048=CECT 7243).

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2008-06-01
2019-10-21
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vol. , part 6, pp. 1346 - 1349

Cellular fatty acid composition (%) for strains M3B and M3T and their closest phylogenetic neighbours

. Levels of DNA–DNA relatedness among strains

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