1887

Abstract

Two Gram-negative, cold-adapted, moderately halophilic, aerobic bacteria, designated strains M3B and M3T, were isolated from marine sediment collected from the South Shetland Islands, Antarctica. The organisms were rod-shaped, catalase- and oxidase-positive, and motile by means of polar flagella. These two psychrotolerant strains required Na and grew at NaCl concentrations of 1–15 % and temperatures between 4 and 42 °C. 16S rRNA gene sequence analysis placed strains M3B and M3T within the genus . DNA–DNA hybridization experiments between the Antarctic isolate M3B and type strains of phylogenetically related species, namely , , , , and , revealed levels of relatedness lower than 32 %. Strain M3T showed 99 % DNA relatedness to strain M3B. The DNA G+C contents of M3B and M3T were 57.1 and 57.4 mol%, respectively, and their major isoprenoid quinone was ubiquinone-9. Several phenotypic characteristics, together with data on cellular fatty acid composition, served to differentiate strains M3B and M3T from strains of related species. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains M3B and M3T belong to the same genospecies and represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M3B (=LMG 24048=CECT 7243).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65298-0
2008-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/6/1346.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65298-0&mimeType=html&fmt=ahah

References

  1. Barrow, G. I. & Feltham, R. K. A.(1993).Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  2. Bozal, N., Montes, M. J., Tudela, E., Jiménez, F. & Guinea, J.(2002).Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52, 195–205. [Google Scholar]
  3. Button, D. K. & Robertson, B. R.(2001). Determination of DNA content of aquatic bacteria by flow cytometry. Appl Environ Microbiol 67, 1636–1645.[CrossRef] [Google Scholar]
  4. Cleenwerck, I., Vandemeulebroecke, K., Janssens, D. & Swings, J.(2002). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol 52, 1551–1588.[CrossRef] [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  6. Gauthier, M. J., Lafay, B., Christen, R., Fernandez, L., Acquaviva, M., Bonin, P. & Bertrand, J.-C.(1992).Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42, 568–576.[CrossRef] [Google Scholar]
  7. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K.(1998). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef] [Google Scholar]
  8. Green, D. H., Bowman, J. P., Smith, E. A., Gutierrez, T. & Bolch, C. J. S.(2006).Marinobacter algicola sp. nov., isolated from laboratory cultures of paralytic shellfish toxin-producing dinoflagellates. Int J Syst Evol Microbiol 56, 523–527.[CrossRef] [Google Scholar]
  9. Kim, B.-Y., Weon, H.-Y., Yoo, S.-H., Kim, J.-S., Kwon, S.-W., Stackebrandt, E. & Go, S.-J.(2006).Marinobacter koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 56, 2653–2656.[CrossRef] [Google Scholar]
  10. Martín, S., Márquez, M. C., Sánchez-Porro, C., Mellado, E., Arahal, D. R. & Ventosa, A.(2003).Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity. Int J Syst Evol Microbiol 53, 1383–1387.[CrossRef] [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  12. Niemann, S., Pühler, A., Tichy, H. V., Simon, R. & Selbitschka, W.(1997). Evaluation of the resolving power of the three different fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population. J Appl Microbiol 82, 477–484.[CrossRef] [Google Scholar]
  13. Romanenko, L. A., Schumann, P., Rohde, M., Zhukova, N. V., Mikhailov, V. V. & Stackebrandt, E.(2005).Marinobacter bryozoorum sp. nov. and Marinobacter sediminum sp. nov., novel bacteria from the marine environment. Int J Syst Evol Microbiol 55, 143–148.[CrossRef] [Google Scholar]
  14. Shieh, W. Y., Jean, W. D., Lin, Y. T. & Tseng, M.(2003).Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan. Can J Microbiol 49, 244–252.[CrossRef] [Google Scholar]
  15. Shivaji, S., Gupta, P., Chaturvedi, P., Suresh, K. & Delille, D.(2005).Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 55, 1453–1456.[CrossRef] [Google Scholar]
  16. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  17. Wilson, K.(1987). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Green Publishing & Wiley-Interscience.
  18. Yoon, J.-H., Yeo, S. H., Kim, I.-G. & Oh, T. K.(2004).Marinobacter flavimaris sp. nov. and Marinobacter daepoensis sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54, 1799–1803.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65298-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65298-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1346 - 1349

Cellular fatty acid composition (%) for strains M3B and M3T and their closest phylogenetic neighbours

. Levels of DNA–DNA relatedness among strains

[Single PDF file](82 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error