1887

International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 2

Reclassification of Ying 2007 as gen. nov., comb. nov. and description of sp. nov., a halophilic bacterium isolated from Xinjiang province in China Free

Abstract

A Gram-negative, non-motile and moderately halophilic rod-shaped bacterium, designated strain BH206, was isolated from a saline lake of Xinjiang province in China. The isolate showed catalase-positive and oxidase-negative reactions and did not reduce nitrate. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was most closely related to [] HY1 with 95.8 % 16S rRNA gene sequence similarity, and formed a tight phyletic group with [] HY1 with a bootstrap value of 99 % within the family . However, strain BH206 and [] HY1 formed a phyletic lineage distinct from other species. The 16S rRNA gene sequence similarities of strain BH206 with other related type species were lower than 94.6 %. On the basis of physiological and molecular properties, it is clear that [] should be reclassified in the new genus as gen. nov., comb. nov. (type strain HY1=CGMCC 1.6101=JCM 14015) and that strain BH206 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain of is BH206 (=KCTC 12883=DSM 19287).

  • Published Online:
Keyword(s): ML, maximum-likelihood , MP, maximum-parsimony and NJ, neighbour-joining
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65297-0
2008-02-01
2021-09-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/2/438.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65297-0&mimeType=html&fmt=ahah

References

  1. Bernardet, J. F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  2. Carrasco, I. J., Márquez, M. C., Yanfen, X., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A.(2006).Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. Int J Syst Microbiol 56, 599–604.[CrossRef] [Google Scholar]
  3. Cole, J. R., Chai, B., Marsh, T. L., Farris, R. J., Wang, Q., Kulam, S. A., Chandra, S., McGarrell, D. M., Schmidt, T. M. & other authors(2003). The ribosomal database project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef] [Google Scholar]
  4. Dobson, S. J., Colwell, R. R., Franzmann, P. D. & McMeekin, T. A.(1993). Direct sequencing of the PCR-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43, 77–83.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(2002).phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Science, University of Washington, Seattle, USA. http://evolution.genetics.washington.edu/phylip.html
  6. Gomori, G.(1955). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146. [Google Scholar]
  7. Ivanova, E. P., Bowman, J. P., Christen, R., Zhukova, N. V., Lysenko, A. M., Gorshkova, N. M., Mitik-Dineva, N., Sergeev, A. F. & Mikhailov, V. V.(2006).Salegentibacter flavus sp. nov. Int J Syst Evol Microbiol 56, 583–586.[CrossRef] [Google Scholar]
  8. Jeon, C. O., Lim, J.-M., Lee, J. M., Xu, L. H., Jiang, C. L. & Kim, C.-J.(2005). Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55, 1891–1896.[CrossRef] [Google Scholar]
  9. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  10. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  11. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  12. Lanyi, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  13. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  14. McCammon, S. A. & Bowman, J. P.(2000). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063.[CrossRef] [Google Scholar]
  15. Nedashkovskaya, O. I., Kim, S. B., Han, S. K., Lysenko, A. M., Rohde, M., Zhukova, N. V., Falsen, E., Frolova, G. M., Mikhailov, V. V. & Bae, K. S.(2003).Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. Int J Syst Evol Microbiol 53, 1967–1971.[CrossRef] [Google Scholar]
  16. Nedashkovskaya, O. I., Suzuki, M., Vancanneyt, M., Cleenwerck, I., Zhukova, N. V., Vysotskii, M. V., Mikhailov, V. V. & Swings, J.(2004).Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus. Int J Syst Evol Microbiol 54, 1107–1110.[CrossRef] [Google Scholar]
  17. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Mikhailov, V. V., Bae, K. S. & Kim, I. S.(2005a).Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 55, 235–294.[CrossRef] [Google Scholar]
  18. Nedashkovskaya, O. I., Kim, S. B., Lee, K. H., Mikhailov, V. V. & Bae, K. S.(2005b).Gillisia mitskevichiae sp. nov., a novel bacterium of the family Flavobacteriaceae, isolated from sea water. Int J Syst Evol Microbiol 55, 321–323.[CrossRef] [Google Scholar]
  19. Nedashkovskaya, O. I., Kim, S. B., Vancanney, M., Shin, D. S., Lysenko, A. M., Shevchenko, L. S., Krasokhin, V. B., Mikhailov, V. V., Swings, J. & Bae, K. S.(2006).Salegentibacter agarivorans sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the sponge Artemisina sp. Int J Syst Evol Microbiol 56, 883–887.[CrossRef] [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  21. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  22. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  23. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J.(2004).Gillisia limnaea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a microbial mat in Lake Fryxell, Antarctica. Int J Syst Evol Microbiol 54, 445–448.[CrossRef] [Google Scholar]
  24. Ying, J.-Y., Liu, Z.-P., Wang, B.-J., Dai, X., Yang, S.-S. & Liu, S.-J.(2007).Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter. Int J Syst Evol Microbiol 57, 219–222.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65297-0
Loading
Reclassification of Salegentibacter catena Ying et al. 2007 as Salinimicrobium catena gen. nov., comb. nov. and description of Salinimicrobium xinjiangense sp. nov., a halophilic bacterium isolated from Xinjiang province in China
Int J Syst Evol Microbiol 58, 438 (2008); https://doi.org/10.1099/ijs.0.65297-0
/content/journal/ijsem/10.1099/ijs.0.65297-0
/content/journal/ijsem/10.1099/ijs.0.65297-0
Loading

Data & Media loading...

Supplements

vol. , part 2, pp. 438–442

Transmission electron micrograph showing general morphology of negatively stained cells of strain BH206 .

Cellular fatty acid composition (%) of strain BH206T and related type strains

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error