A bacterial isolate, strain TU-7, with an optimum growth temperature of about 45–50 °C and an optimum pH for growth between 8.0 and 8.5, was recovered from a hot spring in the Furnas area of the island of São Miguel in the Azores. The new isolate is non-pigmented, facultatively chemolithoorganotrophic, strictly aerobic and catalase- and oxidase-positive. The organism oxidized thiosulfate to sulfate with enhancement of growth. This organism assimilated organic acids but did not assimilate carbohydrates or polyols. 16S rRNA gene sequence analysis placed strain TU-7 within the radiation of the as a deep branch of the family . The major fatty acids of strain TU-7 are 18 : 17, 18 : 0, 19 : 0 cyclo 8 and an aliphatic chain with an equivalent chain-length of 20.195, identified as 19 : 0 2-OH cyclo 8 (Δ11 : 12). Ubiquinone 10 is the major respiratory quinone and the major polar lipids are phosphatidylcholine, phosphatidylethanolamine, diphosphosphatidylglycerol and phosphatidylglycerol in addition to two unidentified aminolipids. Bacteriochlorophyll and genes were not detected. Based on 16S rRNA gene sequence analysis and physiological and biochemical characteristics, we describe a novel species of a new genus represented by strain TU-7, for which we propose the name gen. nov., sp. nov. The type strain of is strain TU-7 (=DSM 17972 =CIP 109115).


Article metrics loading...

Loading full text...

Full text loading...



  1. Alarico, S., Rainey, F. A., Empadinhas, N., Schumann, P., Nobre, M. F. & da Costa, M. S.(2002).Rubritepida flocculans gen. nov., sp. nov., a new slightly thermophilic member of the α-1 subclass of the Proteobacteria. Syst Appl Microbiol 25, 198–206.[CrossRef] [Google Scholar]
  2. Albuquerque, L., Santos, J., Travassos, P., Nobre, M. F., Rainey, F. A., Wait, R., Empadinhas, N., Silva, M. T. & da Costa, M. S.(2002).Albidovulum inexpectatum gen. nov., sp. nov., a non-photosynthetic and slightly thermophilic bacterium from a marine hot spring that is very closely related to the photosynthetic genus Rhodovulum. Appl Environ Microbiol 68, 4266–4273.[CrossRef] [Google Scholar]
  3. Albuquerque, L., Simões, C., Nobre, M. F., Pino, N. M., Battista, J. R., Silva, M. T., Rainey, F. A. & da Costa, M. S.(2005).Truepera radiovictrix gen. nov., sp. nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov. FEMS Microbiol Lett 247, 161–169.[CrossRef] [Google Scholar]
  4. Cole, J. R., Chain, B., Marsh, T. L., Farris, R. J., Wang, Q., Kulam, S. A., Chandra, S., McGarrell, D. M., Schmidt, T. M. & other authors(2003). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef] [Google Scholar]
  5. Degryse, E., Glansdorff, N. & Pierard, A.(1978). A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch Microbiol 117, 189–196.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. França, L., Rainey, F. A., Nobre, M. F. & da Costa, M. S.(2006).Tepidicella xavieri gen. nov., sp. nov., a betaproteobacterium isolated from a hot spring runoff. Int J Syst Evol Microbiol 56, 907–912.[CrossRef] [Google Scholar]
  8. Hanada, S., Kawase, Y., Hiraishi, A., Takaichi, S., Matsuura, K., Shimada, K. & Nagashima, K. V. P.(1997).Porphyrobacter tepidarius sp. nov., a moderately thermophilic aerobic photosynthetic bacterium isolated from a hot spring. Int J Syst Bacteriol 47, 408–413.[CrossRef] [Google Scholar]
  9. Heimbrook, M. E., Wang, W. L. L. & Campbell, G.(1989). Staining bacterial flagella easily. J Clin Microbiol 27, 2612–2615. [Google Scholar]
  10. Hiraishi, A.(2005). Genus IV. Acidocella Kishimoto, Kosako, Wakao, Tano, and Hiraishi 1996, 362VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 65–68. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  11. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  12. Liaaen-Jensen, S. & Jensen, A.(1971). Quantitative determination of carotenoids in photosynthetic tissues. Methods Enzymol 23, 586–602. [Google Scholar]
  13. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  15. Moreira, C., Rainey, F. A., Nobre, M. F., Silva, M. T. & da Costa, M. S.(2000).Tepidimonas ignava gen. nov., sp. nov., a new chemolithoheterotrophic and slightly thermophilic member of the β-Proteobacteria. Int J Syst Evol Microbiol 50, 735–742.[CrossRef] [Google Scholar]
  16. Nielsen, P., Fritze, D. & Priest, F. G.(1995). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141, 1745–1761.[CrossRef] [Google Scholar]
  17. Pires, A. L., Albuquerque, L., Tiago, I., Nobre, M. F., Empadinhas, N., Veríssimo, A. & da Costa, M.(2005).Meiothermus timidus sp. nov., a slightly thermophilic yellow-pigmented species. FEMS Microbiol Lett 245, 39–45.[CrossRef] [Google Scholar]
  18. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  19. Rainey, F. A., Silva, J., Nobre, M. F., Silva, M. T. & da Costa, M. S.(2003).Porphyrobacter cryptus sp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a-containing species. Int J Syst Evol Microbiol 53, 35–41.[CrossRef] [Google Scholar]
  20. Rathgeber, C. & Yurkov, V. V.(2005). Genus VII. Craurococcus Saitoh, Suzuki and Nishimura 1998, 1044VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 70–72. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  21. Reasoner, D. J. & Geldreich, E. E.(1985). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49, 1–7. [Google Scholar]
  22. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  23. Sievers, M. & Swings, J.(2005). Genus I. Acetobacter Beijerinck 1898, 215AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 51–54. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  24. Sörbo, B.(1987). Sulfate: turbidimetric and nephelometric methods. Methods Enzymol 143, 3–6. [Google Scholar]
  25. Tindall, B. J.(1989). Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum. FEMS Microbiol Lett 60, 251–254.[CrossRef] [Google Scholar]
  26. Westley, J.(1987). Thiocyanate and thiosulfate. Methods Enzymol 143, 23–25. [Google Scholar]
  27. Williams, R. A. D. & da Costa, M. S.(1992). The genus Thermus and related microorganisms. In The Prokaryotes, 2nd edn, pp. 3745–3753. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  28. Yurkov, V. V.(2005). Genus XII. Roseococcus Yurkov, Stackebrandt, Holmes, Fuerst, Hugenholtz, Golecki, Gad'on, Gorlenko, Kompantseva and Drews 1994c, 430VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 85–87. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  29. Zhang, G., Dong, H., Xu, Z., Zhao, D. & Zhang, C.(2005). Microbiol diversity in ultra-high-pressure rocks and fluids from the Chinese continental scientific drilling project in China. Appl Environ Microbiol 71, 3213–3227.[CrossRef] [Google Scholar]

Data & Media loading...


One-dimensional TLC of polar lipids of strains TU-7 , H-8 and FRR-10 . Lipids were stained by spraying with 5 % molydbatophosphoric acid in ethanol followed by heating at 160 °C. DPG, Diphosphatidylglycerol; PE, phosphatidylethnolamine; PG, phosphatidylglycerol; PC, phosphatidylcholine; AL, aminolipid.


Mean fatty acid composition of strain TU-7 grown at 50 °C. [PDF](73 KB)


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error