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Abstract

A bacterial isolate, strain TU-7, with an optimum growth temperature of about 45–50 °C and an optimum pH for growth between 8.0 and 8.5, was recovered from a hot spring in the Furnas area of the island of São Miguel in the Azores. The new isolate is non-pigmented, facultatively chemolithoorganotrophic, strictly aerobic and catalase- and oxidase-positive. The organism oxidized thiosulfate to sulfate with enhancement of growth. This organism assimilated organic acids but did not assimilate carbohydrates or polyols. 16S rRNA gene sequence analysis placed strain TU-7 within the radiation of the as a deep branch of the family . The major fatty acids of strain TU-7 are 18 : 17, 18 : 0, 19 : 0 cyclo 8 and an aliphatic chain with an equivalent chain-length of 20.195, identified as 19 : 0 2-OH cyclo 8 (Δ11 : 12). Ubiquinone 10 is the major respiratory quinone and the major polar lipids are phosphatidylcholine, phosphatidylethanolamine, diphosphosphatidylglycerol and phosphatidylglycerol in addition to two unidentified aminolipids. Bacteriochlorophyll and genes were not detected. Based on 16S rRNA gene sequence analysis and physiological and biochemical characteristics, we describe a novel species of a new genus represented by strain TU-7, for which we propose the name gen. nov., sp. nov. The type strain of is strain TU-7 (=DSM 17972 =CIP 109115).

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2008-04-01
2019-10-14
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One-dimensional TLC of polar lipids of strains TU-7 , H-8 and FRR-10 . Lipids were stained by spraying with 5 % molydbatophosphoric acid in ethanol followed by heating at 160 °C. DPG, Diphosphatidylglycerol; PE, phosphatidylethnolamine; PG, phosphatidylglycerol; PC, phosphatidylcholine; AL, aminolipid.

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Mean fatty acid composition of strain TU-7 grown at 50 °C. [PDF](73 KB)

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