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Abstract

An aerobic, Gram-positive, non-motile, non-spore-forming, rod–coccus-shaped bacterium, strain 5317S-21, was isolated from an air sample from Suwon city, Republic of Korea. The isolate was able to grow within a pH range of 5.0–9.0 and a temperature range of 5–35 °C and it tolerated up to 2 % (w/v) NaCl. The cell-wall peptidoglycan contained -diaminopimelic acid as diagnostic diamino acid. The predominant isoprenoid quinone was MK-8(H). The major polar lipids were phosphatidylinositol, phosphatidylethanolamine and diphosphatidylglycerol; phosphatidylglycerol and several unknown phospholipids were also detected. Mycolic acids were absent. The only whole-cell sugar was glucose. The major cellular fatty acids were iso-C, C 8 and iso-C. 16S rRNA gene sequence analysis indicated that strain 5317S-21 was related phylogenetically to members of the genus , with 97.4 % sequence similarity to the type strains of and . The G+C content of the genomic DNA of strain 5317S-21 was 73 mol%. Levels of DNA–DNA relatedness between strain 5317S-21 and the type strains of and were 37 and 41 %, respectively. It was concluded that strain 5317S-21 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5317S-21 (=KACC 20583 =DSM 18566).

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2007-12-01
2019-10-15
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Polar lipid profiles of strain 5317S-21 , DSM 12331 and DSM 12332 after separation by two-dimensional TLC. [PDF](44 KB)

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Cellular fatty acid compositions of members of the genus . [PDF](37 KB)

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