RT Journal Article SR Electronic(1) A1 De Bruyne, Katrien A1 Schillinger, Ulrich A1 Caroline, Lily A1 Boehringer, Benjamin A1 Cleenwerck, Ilse A1 Vancanneyt, Marc A1 De Vuyst, Luc A1 Franz, Charles M. A. P. A1 Vandamme, PeterYR 2007 T1 Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 12 SP 2952 OP 2959 DO https://doi.org/10.1099/ijs.0.65292-0 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, ovoid lactic acid bacterium, strain LMG 23990T, was isolated from Ethiopian coffee fermentation. 16S rRNA gene sequence analysis indicated that the novel strain belongs to the genus Leuconostoc, with Leuconostoc citreum and Leuconostoc lactis as the closest neighbours (99.6 and 99.0 % 16S rRNA gene sequence similarity, respectively). Genotypic fingerprinting by fluorescent amplified fragment length polymorphism, whole-cell protein electrophoresis, DNA–DNA hybridizations, comparative sequence analysis of pheS, rpoA, atpA, and physiological and biochemical tests allowed us to differentiate strain LMG 23990T from all established Leuconostoc species. Strain LMG 23990T (=CCUG 54536T) therefore represents a novel species, for which the name Leuconostoc holzapfelii sp. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65292-0