A Gram-negative, non-spore-forming, rod-shaped bacterial strain, K106T, was isolated from wastewater collected from a textile dye works in Korea. Strain K106T grew optimally at pH 7.0–7.5 and 30–37 °C in the presence of 0–1.0 % (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed that strain K106T joined the type strain of Chelatococcus asaccharovorans with a bootstrap resampling value of 99.9 %. The predominant ubiquinone of strain K106T was Q-10. The fatty acid profile of strain K106T was similar to that of C. asaccharovorans DSM 6462T. Major polar lipids of strain K106T and C. asaccharovorans DSM 6462T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two aminolipids and two unidentified phospholipids. sym-Homospermidine, spermidine and putrescine were major polyamines. The DNA G+C content was 68.3 mol%. Strain K106T exhibited 16S rRNA gene sequence similarity of 96.6 % to the type strain of C. asaccharovorans. DNA–DNA relatedness data and differential phenotypic properties, particularly differences in cell morphology and the ability to utilize nitrilotriacetate, demonstrated that strain K106T can be differentiated from C. asaccharovorans. On the basis of phenotypic, phylogenetic and genetic data, strain K106T represents a novel species of the genus Chelatococcus, for which the name Chelatococcus daeguensis sp. nov. is proposed. The type strain is K106T (=KCTC 12979T =CCUG 54519T).
Auling, G., Busse, H.-J., Egli, T., El-Banna, T. & Stackebrandt, E.(1993). Description of the Gram-negative, obligately aerobic, nitrilotriacetate (NTA)-utilizing bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asaccharovorans, gen. nov., sp. nov. Syst Appl Microbiol16, 104–112.
[Google Scholar]
Busse, H.-J. & Auling, G.(1988). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol11, 1–8.[CrossRef][Google Scholar]
Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
Egli, T., Weilenmann, H.-U., El-Banna, T. & Auling, G.(1988). Gram-negative, aerobic, nitrilotriacetate-utilizing bacteria from wastewater and soil. Syst Appl Microbiol10, 297–305.[CrossRef][Google Scholar]
Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39, 224–229.[CrossRef][Google Scholar]
Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol19, 161–207.
[Google Scholar]
Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol19, 1–67.
[Google Scholar]
Lee, K.-B., Liu, C.-T., Anzai, Y., Kim, H., Aono, T. & Oyaizu, H.(2005). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol55, 1907–1919.[CrossRef][Google Scholar]
Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2, 233–241.[CrossRef][Google Scholar]
Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note no. 101. Newark, DE: MIDI.
Schenkel, E., Berlaimont, V., Dubois, J., Helson-Cambier, M. & Hanocq, M.(1995). Improved high-performance liquid chromatographic method for the determination of polyamines as their benzoylated derivatives: application to P388 cancer cells. J Chromatogr B Biomed Appl668, 189–197.[CrossRef][Google Scholar]
Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol44, 846–849.[CrossRef][Google Scholar]
Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25, 125–128.[CrossRef][Google Scholar]
Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37, 463–464.[CrossRef][Google Scholar]
Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol46, 502–505.[CrossRef][Google Scholar]
Yoon, J.-H., Lee, S. T. & Park, Y.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol48, 187–194.[CrossRef][Google Scholar]
Yoon, J.-H., Kang, K. H. & Park, Y.-H.(2003).Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol53, 449–454.[CrossRef][Google Scholar]