The taxonomic position of a novel bacterial strain, YC5194, with antimicrobial activity, isolated from the rhizosphere of pepper in Jinju, South Korea, was studied using a polyphasic approach. Cells of the strain were Gram-negative, rod-shaped, facultative anaerobes. It grew at a temperature of 15–37 °C (optimum 28 °C). Growth of the strain occurred between pH 5.5 and 8.5, with an optimum of pH 7.0–7.5. The strain inhibited mycelial growth of , , , , and and growth of . The G+C content of the total DNA was 65.4 mol%. The 16S rRNA gene sequence of the strain was most closely related to species of the genus (<94.0 to >99.0 % sequence similarity). Chemotaxonomic data (major quinone, Q-8; major polar lipids, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyl--methylethanolamine; major fatty acids, iso-C, summed feature 3, C, iso-C 9 and C 7) supported the affiliation of strain YC5194 to the genus . Phylogenetic analysis based on 16S rRNA gene sequences, DNA–DNA hybridization data and biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain YC5194 from species of with validly published names. Strain YC5194 therefore represents a novel species, for which the name sp. nov. is proposed. The type strain is YC5194 (=KCTC 22007 =DSM 19286).


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[PDF file of Supplementary Tables S1-S3](149 KB)


[PDF file of Supplementary Tables S1-S3](149 KB)


Transmission electron micrographs of cells of sp. nov. YC5194 in a 3 day-old culture cultivated on 0.1 % TSA.

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