1887

Abstract

A moderately halophilic, Gram-negative bacterium, designated strain BH843, was isolated from a solar saltern in Korea and subjected to a taxonomic analysis. Strain BH843 grew at salinities of 1–14 % (w/v) NaCl and at temperatures of 10–40 °C. The cells were motile cocci or short rods with single flagella and contained C, C cyclo 8 and C cyclo as the major fatty acids. The G+C content of the genomic DNA was 66 mol% and the predominant ubiquinone was Q-9. Comparative 16S rRNA gene sequence analyses showed that strain BH843 formed a distinct phyletic line within the genus , and the levels of 16S rRNA gene sequence similarity with respect to recognized species were below 95.1 %. The levels of DNA–DNA relatedness between strain BH843 and the type strains of phylogenetically closely related species were below 25 %. On the basis of phenotypic, chemotaxonomic and molecular data, strain BH843 represents a novel species within the genus , for which the name is proposed. The type strain is BH843 (=KCTC 12584=DSM 17892).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65285-0
2007-10-01
2024-11-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/10/2194.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65285-0&mimeType=html&fmt=ahah

References

  1. Arahal D. R., García M. T., Ludwig W., Schleifer K. H., Ventosa A. 2001; Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51:1443–1448
    [Google Scholar]
  2. Arahal D. R., Castillo A. M., Ludwig W., Schleifer K. H., Ventosa A. 2002a; Proposal of Cobetia marina gen. nov., comb. nov. within the family Halomonadaceae , to include the species Halomonas marina . Syst Appl Microbiol 25:207–211 [CrossRef]
    [Google Scholar]
  3. Arahal D. R., Ludwig W., Schleifer K. H., Ventosa A. 2002b; Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. Int J Syst Evol Microbiol 52:241–249
    [Google Scholar]
  4. Baumann L., Bowditch R. D., Baumann P. 1983; Description of Deleya gen. nov. created to accommodate the marine species Alcaligenes aestus , A. pacificus , A. cupidus , A. venustus , and Pseudomonas marina . Int J Syst Bacteriol 33:793–802 [CrossRef]
    [Google Scholar]
  5. Berendes F., Gottschalk G., Heine-Dobbernack E., Moore E. R. B., Tindall B. J. 1996; Halomonas desiderata sp. nov., a new alkaliphilic, halotolerant and denitrifying bacterium isolated from a municipal sewage works. Syst Appl Microbiol 19:158–167 [CrossRef]
    [Google Scholar]
  6. Bouchotroch S., Quesada E., Moral A. D., Llamas I., Béjar V. 2001; Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 51:1625–1632 [CrossRef]
    [Google Scholar]
  7. Christensen H., Bisgaard M., Frederiksen W., Mutters R., Kuhnert P., Olsen J. E. 2001; Is characterization of a single isolate sufficient for valid publication of a new genus or species? Proposal to modify recommendation 30b of the Bacteriological Code (1990 Revision. Int J Syst Evol Microbiol 51:2221–2225 [CrossRef]
    [Google Scholar]
  8. Cole J. R., Chai B., Marsh T. L., Farris R. J., Wang Q., Kulam S. A., Chandra S., McGarrell D. M., Schmidt T. M. other authors 2003; The ribosomal database project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31:442–443 [CrossRef]
    [Google Scholar]
  9. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  10. Dobson S. J., Franzmann P. D. 1996; Unification of the genera Deleya (Baumann et al . 1983), Halomonas (Vreeland et al . 1980),and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas , and placement of the genus Zymobacter in the family Halomonadaceae . Int J Syst Bacteriol 46:550–558 [CrossRef]
    [Google Scholar]
  11. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  12. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  13. Franzmann P. D., Wehmeyer U., Stackebrandt E. 1989; Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya . Syst Appl Microbiol 11:16–19
    [Google Scholar]
  14. Garcia M. T., Mellado E., Ostos J. C., Ventosa A. 2004; Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. Int J Syst Evol Microbiol 54:1723–1728 [CrossRef]
    [Google Scholar]
  15. Garriga M., Ehrmann M. A., Arnau J., Hugas M., Vogel R. F. 1998; Carnimonas nigrificans gen. nov., sp. nov. a bacterial causative agent for black spot formation on cured meat products. Int J Syst Bacteriol 48:677–686 [CrossRef]
    [Google Scholar]
  16. Gomori G. 1955; Preparation of buffers for use in enzyme studies. In Methods in Enzymology vol 1 pp 138–146 Edited by Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  17. Heyrman J., Balcaen A., Vos P. D., Swings J. 2002; Halomonas muralis sp. nov., isolated from microbial biofilms colonizing the walls and murals of the Saint-Catherine chapel (Castle Herberstein, Austria). Int J Syst Evol Microbiol 52:2049–2054 [CrossRef]
    [Google Scholar]
  18. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  19. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  20. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  21. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  22. Lee E. M., Jeon C. O., Choi I., Chang K. S., Kim C.-J. 2005; Silanimonas lenta gen. nov., sp. nov. a slightly thermophilic and alkaliphilic gammaproteobacterium isolated from a hot spring. Int J Syst Evol Microbiol 55:385–389 [CrossRef]
    [Google Scholar]
  23. Lee J. C., Jeon C. O., Lim J. M., Lee S. M., Lee J. M., Song S. M., Park D. J., Li W. J., Kim C.-J. 2005; Halomonas taeanensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 55:2027–2032 [CrossRef]
    [Google Scholar]
  24. Lim J.-M., Yoon J. H., Lee J. C., Jeon C. O., Park D. J., Sung C. K., Kim C.-J. 2004; Halomonas koreensis sp. nov., a moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 54:2037–2042 [CrossRef]
    [Google Scholar]
  25. Martínez-Cánovas M. J., Béjar V., Martínez-Checa F., Quesada E. 2004; Halomonas anticariensis sp. nov., from Fuente de Piedra, a saline-wetland wildfowl reserve in Málaga, southern Spain. Int J Syst Evol Microbiol 54:1329–1332 [CrossRef]
    [Google Scholar]
  26. Mata J. A., Martínez-Cánovas M. J., Quesada E., Béjar V. 2002; A detailed phenotypic characterization of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375 [CrossRef]
    [Google Scholar]
  27. Mellado E., Moore E. R., Nieto J. J., Ventosa A. 1995; Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina , and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45:712–716 [CrossRef]
    [Google Scholar]
  28. Poli A., Esposito E., Orlando P., Lama L., Giordano A., de Appolonia F., Nicolaus B., Gambacorta A. 2007; Halomonas alkaliantarctica sp. nov., isolated from saline lake Cape Russell in Antarctica, an alkalophilic moderately halophilic, exopolysaccharide-producing bacterium. Syst Appl Microbiol 30:31–38 [CrossRef]
    [Google Scholar]
  29. Romano I., Nicolaus B., Lama L., Manca M. C., Gambacorta A. 1996; Characterization of a haloalkalophilic strictly aerobic bacterium, isolated from Pantelleria island. Syst Appl Microbiol 19:326–333 [CrossRef]
    [Google Scholar]
  30. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [CrossRef]
    [Google Scholar]
  31. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
  32. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  33. Teles F., Haffajee A. D., Socransky S. S. 2007; Multiple displacement amplification as an aid in checkerboard DNA-DNA hybridization. Oral Microbiol Immunol 22:118–125 [CrossRef]
    [Google Scholar]
  34. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  35. Ventosa A., Nieto J. J., Oren A. 1998; Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62:504–544
    [Google Scholar]
  36. Vreeland R. H., Litchfield C. D., Martin E. L., Elliot E. 1980; Halomonas elongata , a new genus and species of extremely salt tolerant bacteria. Int J Syst Bacteriol 30:485–495 [CrossRef]
    [Google Scholar]
  37. Yakimov M. M., Golyshin P. N., Lang S., Moore E. R., Abraham W. R., Lunsdorf H., Timmis K. N. 1998; Alcanivorax borkumensis gen. nov., sp. nov., a new hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 48:339–348 [CrossRef]
    [Google Scholar]
  38. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  39. Yoon J.-H., Lee K.-C., Kho Y.-H., Kang K.-H., Kim C.-J., Park Y.-H. 2002; Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52:123–130
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.65285-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65285-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error