sp. nov., isolated from soil Free

Abstract

A Gram-positive, non-motile, coccoid-shaped bacterium, designated strain DS-8, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic study. Strain DS-8 grew optimally at 30 °C and pH 6.5–7.5 in the presence of 0.5–1.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain DS-8 joined the type strain of , with a bootstrap resampling value of 92.5 %, and shared 96.5 % 16S rRNA gene sequence similarity with . The cell-wall peptidoglycan type was based on -diaminopimelic acid and the acyl type of the muramic acid was acetyl. The predominant menaquinone was MK-8(H). The major fatty acids were iso-C and iso-C. Major polar lipids detected in strain DS-8 were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 70.7 mol%. DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain DS-8 differs from . On the basis of the data obtained, strain DS-8 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-8 (=KCTC 19248=CCUG 54521).

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2008-03-01
2024-03-28
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