RT Journal Article SR Electronic(1) A1 Lee, Dong Wan A1 Lee, Jin Mi A1 Seo, Jae Pyo A1 Schumann, Peter A1 Kim, Sae Jae A1 Lee, Soon DongYR 2008 T1 Phycicola gilvus gen. nov., sp. nov., an actinobacterium isolated from living seaweed JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 6 SP 1318 OP 1323 DO https://doi.org/10.1099/ijs.0.65283-0 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, aerobic, non-mycelium-forming actinomycete, designated strain SSWW-21T, was isolated from a living seaweed sample on the coast of Jeju, Republic of Korea. Cells of the organism were rod-shaped or coccoid depending on culture age. Short rods were observed in young cultures, whereas older cultures predominantly consisted of coccoid cells. Rod-shaped cells were motile by means of flagella, but coccoid cells were non-motile. Budding-like cell division was observed. The temperature and pH for growth were 4–30 °C and pH 6.1–10.1. Growth occurred in the presence of up to 2 % NaCl. Phylogenetic analyses showed that the organism formed a distinct clade within the family Microbacteriaceae. Comparative 16S rRNA gene sequence analyses showed that strain SSWW-21T was related most closely to the type strains of Okibacterium fritillariae (96.8 % similarity), Leifsonia poae (96.8 %) and Agreia bicolorata (96.6 %). Levels of 16S rRNA gene sequence similarity between the new isolate and members of other genera within the family Microbacteriaceae were in the range 93.1–96.3 %. The cell-wall peptidoglycan of strain SSWW-21T was of the B-type (2,4-diaminobutyric acid as the diagnostic diamino acid). The acyl type of the muramic acid was acetyl. The predominant menaquinone was MK-11. The polar lipid profile contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and diphosphatidylglycerol. Mycolic acids were not present. The major cellular fatty acids were saturated branched-chain components (anteiso-C15 : 0 and anteiso-C17 : 0). The G+C content of the DNA was 69.8 mol%. On the basis of the phenotypic and phylogenetic data presented, strain SSWW-21T is considered to represent a novel species of a new genus within the family Microbacteriaceae, for which the name Phycicola gilvus gen. nov., sp. nov. is proposed. The type strain of Phycicola gilvus is SSWW-21T (=KCTC 19185T =DSM 18319T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65283-0