@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65283-0, author = "Lee, Dong Wan and Lee, Jin Mi and Seo, Jae Pyo and Schumann, Peter and Kim, Sae Jae and Lee, Soon Dong", title = "Phycicola gilvus gen. nov., sp. nov., an actinobacterium isolated from living seaweed", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "6", pages = "1318-1323", doi = "https://doi.org/10.1099/ijs.0.65283-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65283-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "DAB, 2,4-diaminobutyric acid", abstract = "A Gram-positive, aerobic, non-mycelium-forming actinomycete, designated strain SSWW-21T, was isolated from a living seaweed sample on the coast of Jeju, Republic of Korea. Cells of the organism were rod-shaped or coccoid depending on culture age. Short rods were observed in young cultures, whereas older cultures predominantly consisted of coccoid cells. Rod-shaped cells were motile by means of flagella, but coccoid cells were non-motile. Budding-like cell division was observed. The temperature and pH for growth were 4–30 °C and pH 6.1–10.1. Growth occurred in the presence of up to 2 % NaCl. Phylogenetic analyses showed that the organism formed a distinct clade within the family Microbacteriaceae. Comparative 16S rRNA gene sequence analyses showed that strain SSWW-21T was related most closely to the type strains of Okibacterium fritillariae (96.8 % similarity), Leifsonia poae (96.8 %) and Agreia bicolorata (96.6 %). Levels of 16S rRNA gene sequence similarity between the new isolate and members of other genera within the family Microbacteriaceae were in the range 93.1–96.3 %. The cell-wall peptidoglycan of strain SSWW-21T was of the B-type (2,4-diaminobutyric acid as the diagnostic diamino acid). The acyl type of the muramic acid was acetyl. The predominant menaquinone was MK-11. The polar lipid profile contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and diphosphatidylglycerol. Mycolic acids were not present. The major cellular fatty acids were saturated branched-chain components (anteiso-C15 : 0 and anteiso-C17 : 0). The G+C content of the DNA was 69.8 mol%. On the basis of the phenotypic and phylogenetic data presented, strain SSWW-21T is considered to represent a novel species of a new genus within the family Microbacteriaceae, for which the name Phycicola gilvus gen. nov., sp. nov. is proposed. The type strain of Phycicola gilvus is SSWW-21T (=KCTC 19185T =DSM 18319T).", }