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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 6

gen. nov., sp. nov., an actinobacterium isolated from living seaweed Free

Abstract

A Gram-positive, aerobic, non-mycelium-forming actinomycete, designated strain SSWW-21, was isolated from a living seaweed sample on the coast of Jeju, Republic of Korea. Cells of the organism were rod-shaped or coccoid depending on culture age. Short rods were observed in young cultures, whereas older cultures predominantly consisted of coccoid cells. Rod-shaped cells were motile by means of flagella, but coccoid cells were non-motile. Budding-like cell division was observed. The temperature and pH for growth were 4–30 °C and pH 6.1–10.1. Growth occurred in the presence of up to 2 % NaCl. Phylogenetic analyses showed that the organism formed a distinct clade within the family . Comparative 16S rRNA gene sequence analyses showed that strain SSWW-21 was related most closely to the type strains of (96.8 % similarity), (96.8 %) and (96.6 %). Levels of 16S rRNA gene sequence similarity between the new isolate and members of other genera within the family were in the range 93.1–96.3 %. The cell-wall peptidoglycan of strain SSWW-21 was of the B-type (2,4-diaminobutyric acid as the diagnostic diamino acid). The acyl type of the muramic acid was acetyl. The predominant menaquinone was MK-11. The polar lipid profile contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and diphosphatidylglycerol. Mycolic acids were not present. The major cellular fatty acids were saturated branched-chain components (anteiso-C and anteiso-C). The G+C content of the DNA was 69.8 mol%. On the basis of the phenotypic and phylogenetic data presented, strain SSWW-21 is considered to represent a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is SSWW-21 (=KCTC 19185 =DSM 18319).

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Keyword(s): DAB, 2,4-diaminobutyric acid
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2008-06-01
2024-04-24
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Phycicola gilvus gen. nov., sp. nov., an actinobacterium isolated from living seaweed
Int J Syst Evol Microbiol 58, 1318 (2008); https://doi.org/10.1099/ijs.0.65283-0
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