1887

Abstract

A Gram-positive, non-motile, rod-shaped bacterial strain, DS-30, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain DS-30 grew optimally at pH 6.0–7.0 and 25 °C in the presence of 0–0.5 % (w/v) NaCl. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-30 fell within the genus , forming a coherent cluster with the type strains of and , with which it exhibited 16S rRNA gene sequence similarity values of 98.1 and 98.3 %, respectively. Strain DS-30 exhibited 16S rRNA gene sequence similarity values below 96.8 % with respect to the type strains of the other species. The chemotaxonomic properties of strain DS-30 were consistent with those of the genus : the cell-wall peptidoglycan type was based on -diaminopimelic acid, the predominant menaquinone was MK-8(H) and the major fatty acids were iso-C, C 8 and C 9. The DNA G+C content was 71.3 mol%. Strain DS-30 exhibited DNA–DNA relatedness levels of 19 and 22 % with respect to the type strains of . and . , respectively, and could be differentiated from these species with reference to a number of phenotypic characteristics. On the basis of the data obtained, strain DS-30 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-30 (=KCTC 19246 =CCUG 54522).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65267-0
2008-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/2/434.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65267-0&mimeType=html&fmt=ahah

References

  1. Choi, D. H., Kim, H. M., Noh, J.-H. & Cho, B. C.(2007).Nocardioides marinus sp. nov. Int J Syst Evol Microbiol 57, 775–779.[CrossRef] [Google Scholar]
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  3. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  4. Kubota, M., Kawahara, K., Sekiya, K., Uchida, T., Hattori, Y., Futamata, H. & Hiraishi, A.(2005).Nocardioides aromaticivorans sp. nov., a dibenzofuran-degrading bacterium isolated from dioxin-polluted environments. Syst Appl Microbiol 28, 165–174.[CrossRef] [Google Scholar]
  5. Lawson, P. A., Collins, M. D., Schumann, P., Tindall, B. J., Hirsch, P. & Labrenz, M.(2000). New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella lacustris sp. nov. Syst Appl Microbiol 23, 219–229.[CrossRef] [Google Scholar]
  6. Lee, S. D.(2007).Nocardioides furvisabuli sp. nov., isolated from black sand. Int J Syst Evol Microbiol 57, 35–39.[CrossRef] [Google Scholar]
  7. Prauser, H.(1976).Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 58–65.[CrossRef] [Google Scholar]
  8. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  9. Tamura, T. & Yokota, A.(1994). Transfer of Nocardioides fastidiosa Collins and Stackebrandt 1989 to the genus Aeromicrobium as Aeromicrobium fastidiosum comb. nov. Int J Syst Bacteriol 44, 608–611.[CrossRef] [Google Scholar]
  10. Urzì, C., Salamone, P., Schumann, P. & Stackebrandt, E.(2000).Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. Int J Syst Evol Microbiol 50, 529–536.[CrossRef] [Google Scholar]
  11. Wang, Y. M., Zhang, Z. S., Xu, X. L., Ruan, J. S. & Wang, Y.(2001).Actinopolymorpha singaporensis gen. nov., sp. nov., a novel actinomycete from the tropical rainforest of Singapore. Int J Syst Evol Microbiol 51, 467–473. [Google Scholar]
  12. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  13. Yi, H. & Chun, J.(2004).Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 1295–1299.[CrossRef] [Google Scholar]
  14. Yoon, J.-H., Rhee, S.-K., Lee, J.-S., Park, Y.-H. & Lee, S. T.(1997).Nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column. Int J Syst Bacteriol 47, 933–938.[CrossRef] [Google Scholar]
  15. Yoon, J.-H., Cho, Y.-G., Lee, S. T., Suzuki, K., Nakase, T. & Park, Y.-H.(1999).Nocardioides nitrophenolicus sp. nov., a p-nitrophenol-degrading bacterium. Int J Syst Bacteriol 49, 675–680.[CrossRef] [Google Scholar]
  16. Yoon, J.-H., Kim, I.-G., Kang, K. H., Oh, T.-K. & Park, Y.-H.(2004).Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. Int J Syst Evol Microbiol 54, 71–75.[CrossRef] [Google Scholar]
  17. Yoon, J.-H., Kim, I.-G., Lee, M.-H., Lee, C.-H. & Oh, T.-K.(2005a).Nocardioides alkalitolerans sp. nov., isolated from an alkaline serpentinite soil in Korea. Int J Syst Evol Microbiol 55, 809–814.[CrossRef] [Google Scholar]
  18. Yoon, J.-H., Kim, I.-G., Lee, M.-H. & Oh, T.-K.(2005b).Nocardioides kribbensis sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 55, 1611–1614.[CrossRef] [Google Scholar]
  19. Yoon, J.-H., Lee, C.-H. & Oh, T.-K.(2005c).Nocardioides dubius sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 55, 2209–2212.[CrossRef] [Google Scholar]
  20. Yoon, J.-H., Lee, C.-H. & Oh, T.-K.(2006a).Nocardioides lentus sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 56, 271–275.[CrossRef] [Google Scholar]
  21. Yoon, J.-H., Lee, J.-K., Jung, S.-Y., Kim, J.-A., Kim, H.-K. & Oh, T.-K.(2006b).Nocardioides kongjuensis sp. nov., an N-acylhomoserine lactone-degrading bacterium. Int J Syst Evol Microbiol 56, 1783–1787.[CrossRef] [Google Scholar]
  22. Yoon, J.-H., Kang, S.-J., Lee, C.-H. & Oh, T.-K.(2007).Nocardioides insulae sp. nov., isolated from soil. Int J Syst Evol Microbiol 57, 136–140.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65267-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65267-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error