1887

Abstract

A Gram-positive, non-motile, rod-shaped bacterial strain, DS-30, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain DS-30 grew optimally at pH 6.0–7.0 and 25 °C in the presence of 0–0.5 % (w/v) NaCl. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-30 fell within the genus , forming a coherent cluster with the type strains of and , with which it exhibited 16S rRNA gene sequence similarity values of 98.1 and 98.3 %, respectively. Strain DS-30 exhibited 16S rRNA gene sequence similarity values below 96.8 % with respect to the type strains of the other species. The chemotaxonomic properties of strain DS-30 were consistent with those of the genus : the cell-wall peptidoglycan type was based on -diaminopimelic acid, the predominant menaquinone was MK-8(H) and the major fatty acids were iso-C, C 8 and C 9. The DNA G+C content was 71.3 mol%. Strain DS-30 exhibited DNA–DNA relatedness levels of 19 and 22 % with respect to the type strains of . and . , respectively, and could be differentiated from these species with reference to a number of phenotypic characteristics. On the basis of the data obtained, strain DS-30 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-30 (=KCTC 19246 =CCUG 54522).

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2008-02-01
2020-01-23
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