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Abstract

A novel bacterial strain, designated PMB02, was isolated from a leaf of the tree . Colonies grown on TYG agar plates were circular, pink-pigmented and slow-growing, being 0.2–1.5 mm in diameter after 3 days growth. The cells of strain PMB02 were Gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20–30 °C, pH 6–8 and in media containing less than 1 % NaCl. The major respiratory quinone was identified as Q-10. A phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain PMB02 was related to members of the genus . A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species and , with which it showed sequence similarities of 97.7 and 97.4 %, respectively. The values for DNA–DNA hybridization between strain PMB02 and CCM 7218 and GR3 were less than 32 %. On the basis of the phenotypic characterization, the phylogenetic analysis and the DNA–DNA relatedness data, strain PMB02 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PMB02 (=KCTC 12901=JCM 14648).

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2007-12-01
2019-10-20
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References

  1. Anesti, V., Vohra, J., Goonetilleka, S., McDonald, I. R., Sträubler, B., Stackebrandt, E., Kelly, D. P. & Wood, A. P. ( 2004; ). Molecular detection and isolation of facultatively methylotrophic bacteria, including Methylobacterium podarium sp. nov., from the human foot microflora. Environ Microbiol 6, 820–830.[CrossRef]
    [Google Scholar]
  2. Aslam, Z., Lee, C. S., Kim, K.-H., Im, W.-T., Ten, L. N. & Lee, S. T. ( 2007; ). Methylobacterium jeotgali sp. nov., a non-pigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57, 566–571.[CrossRef]
    [Google Scholar]
  3. Bousfield, I. J. & Green, P. N. ( 1985; ). Reclassification of bacteria of the genus Protomonas Urakami and Komagata 1984 in the genus Methylobacterium (Patt, Cole, and Hanson) emend. Green and Bousfield 1983. Int J Syst Bacteriol 35, 209 [CrossRef]
    [Google Scholar]
  4. Corpe, W. A. & Basile, D. V. ( 1982; ). Methanol-utilizing bacteria associated with green plants. Dev Ind Microbiol 23, 483–494.
    [Google Scholar]
  5. Doronina, N. V., Trotsenko, Y. A., Tourova, T. P., Kuznetsov, B. B. & Leisinger, T. ( 2000; ). Methylopila helvetica sp. nov. and Methylobacterium dichloromethanicum sp. nov.: novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane. Syst Appl Microbiol 23, 210–218.[CrossRef]
    [Google Scholar]
  6. Doronina, N. V., Trotsenko, Y. A., Kuznetsov, B. B., Tourova, T. P. & Salkinoja-Salonen, M. S. ( 2002; ). Methylobacterium suomiense sp. nov. and Methylobacterium lusitanum sp. nov., aerobic, pink-pigmented, facultatively methylotrophic bacteria. Int J Syst Evol Microbiol 52, 773–776.[CrossRef]
    [Google Scholar]
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 2002; ). phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Gallego, V., Garcia, M. T. & Ventosa, A. ( 2005a; ). Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 55, 281–287.[CrossRef]
    [Google Scholar]
  10. Gallego, V., Garcia, M. T. & Ventosa, A. ( 2005b; ). Methylobacterium variabile sp. nov., a methylotrophic bacterium isolated from an aquatic environment. Int J Syst Evol Microbiol 55, 1429–1433.[CrossRef]
    [Google Scholar]
  11. Gallego, V., Garcia, M. T. & Ventosa, A. ( 2005c; ). Methylobacterium isbiliense sp. nov., isolated from the drinking water system of Sevilla, Spain. Int J Syst Evol Microbiol 55, 2333–2337.[CrossRef]
    [Google Scholar]
  12. Gallego, V., Garcia, M. T. & Ventosa, A. ( 2006; ). Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water. Int J Syst Evol Microbiol 56, 339–342.[CrossRef]
    [Google Scholar]
  13. Green, P. N. ( 1992; ). The genus Methylobacterium. In The Prokaryotes, 2nd edn, pp. 2342–2349. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  14. Green, P. N. & Bousfield, I. J. ( 1983; ). Emendation of Methylobacterium Patt, Cole, and Hanson 1976; Methylobacterium rhodinum (Heumann 1962) comb. nov. corrig.; Methylobacterium radiotolerans (Ito & Iizuka 1971) comb. nov., corrig.; and Methylobacterium mesophilicum (Austin & Goodfellow 1979) comb. nov. Int J Syst Bacteriol 33, 875–877.[CrossRef]
    [Google Scholar]
  15. Green, P. N., Bousfield, I. J. & Hood, D. ( 1988; ). Three new Methylobacterium species: M. rhodesianum sp. nov., M. zatmanii sp. nov., and M. fujisawaense sp. nov. Int J Syst Bacteriol 38, 124–127.[CrossRef]
    [Google Scholar]
  16. Hirano, S. S. & Upper, C. D. ( 1991; ). Bacterial community dynamics. In Microbial Ecology of Leaves, pp. 271−294. Edited by J. H. Andrews & S. S. Hirano. New York: Springer.
  17. Jourand, P., Giraud, E., Bena, G., Sy, A., Willems, A., Gillis, M., Dreyfus, B. & de Lajudie, P. ( 2004; ). Methylobacterium nodulans sp. nov., for a group of aerobic, facultatively methylotrophic, legume root-nodule-forming and nitrogen-fixing bacteria. Int J Syst Evol Microbiol 54, 2269–2273.[CrossRef]
    [Google Scholar]
  18. Kim, M. K., Im, W.-T., Ohta, H., Lee, M. & Lee, S.-T. ( 2005; ). Sphingopyxis granuli sp. nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in alpha-4 subclass of the Proteobacteria. J Microbiol 43, 152–157.
    [Google Scholar]
  19. Lidstrom, M. E. & Chistoserdova, L. ( 2002; ). Plants in the pink: cytokinin production by Methylobacterium. J Bacteriol 184, 1818 [CrossRef]
    [Google Scholar]
  20. Madhaiyan, M., Kim, B.-Y., Poonguzhali, S., Kwon, S.-W., Song, M.-H., Ryu, J.-H., Go, S.-J., Koo, B.-S. & Sa, T.-M. ( 2007; ). Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice. Int J Syst Evol Microbiol 57, 326–331.[CrossRef]
    [Google Scholar]
  21. McDonald, I. R., Droning, N. V., Trotsenko, Y. A., McAnulla, C. & Murrell, J. C. ( 2001; ). Hyphomicrobium chloromethanicum sp. nov. and Methylobacterium chloromethanicum sp. nov., chloromethane-utilizing bacteria isolated from a polluted environment. Int J Syst Evol Microbiol 51, 119–122.
    [Google Scholar]
  22. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  23. Ostle, A. G. & Holt, J. G. ( 1982; ). Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol 44, 238–241.
    [Google Scholar]
  24. Patt, T. E., Cole, G. C. & Hanson, R. S. ( 1976; ). Methylobacterium, a new genus of facultatively methylotrophic bacteria. Int J Syst Bacteriol 26, 226–229.[CrossRef]
    [Google Scholar]
  25. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  26. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  27. Stanier, R. Y., Palleroni, N. J. & Doudoroff, M. ( 1966; ). The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43, 159–271.[CrossRef]
    [Google Scholar]
  28. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  29. Urakami, T., Araki, H. H., Suzuki, K. & Komogata, K. ( 1993; ). Further studies of the genus Methylobacterium and description of Methylobacterium aminovorans sp. nov. Int J Syst Bacteriol 43, 504–513.[CrossRef]
    [Google Scholar]
  30. Van Aken, B., Peres, C. M., Lafferty-Doty, S., Yoon, J. M. & Schnoor, J. L. ( 2004; ). Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides × nigra DN34). Int J Syst Evol Microbiol 54, 1191–1196.[CrossRef]
    [Google Scholar]
  31. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  32. Wood, A. P., Kelly, D. P., McDonald, I. R., Jordan, S. L., Morgan, T. D., Khan, S., Murrell, J. C. & Borodina, E. ( 1998; ). A novel pink-pigmented facultative methylotroph, Methylobacterium thiocyanatum sp. nov., capable of growth on thiocyanate or cyanate as sole nitrogen sources. Arch Microbiol 169, 148–158.[CrossRef]
    [Google Scholar]
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vol. , part 12, pp. 2849-2853

Level of DNA–DNA hybridization of strain PMB02 and phylogenetically related species of the genus .

Fatty acid content (%) of strain PMB02 and related species of the genus .

Phase-contrast microphotograph of strain PMB02 (a) and polyhydroxyalkanoate-stained cells (b). Yellow granules represent PHAs which are accumulated in the cell.

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