A novel strictly anaerobic, vancomycin-resistant, Gram-positive coccus (strain CCRI-16110T) was isolated from a human faecal specimen. This strain was characterized using morphological, biochemical and molecular taxonomic methods. The organism was unable to hydrolyse aesculin and failed to produce acid from cellobiose, d-lactose and α-raffinose. Acetic acid was the sole product of glucose fermentation by the organism. On the basis of 16S rRNA and tuf gene sequence comparison, strain CCRI-16110T was most closely related to species of the genus Ruminococcus and formed a hitherto unknown sublineage within the Clostridium coccoides rRNA cluster of organisms (cluster XIVa). Based on phenotypic and phylogenetic evidence, a novel species, Ruminococcus gauvreauii sp. nov., is proposed. The type strain is CCRI-16110T (=NML 060141T =CCUG 54292T =JCM 14987T).
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International Journal of Systematic and Evolutionary
Microbiology vol.
58 , part 6, pp. 1393 - 1397
Supplementary Fig. S1. Neighbour-joining phylogenetic
tree based on 16S rRNA gene sequences of strain CCRI-16110
T(
Ruminococcus gauvreauii sp. nov.) and closely related
Gram-positive cocci and bacilli taxa within cluster XIVa (
Clostridium coccoides group) as well as clusters XIVb
and IV (
Clostridium leptum group) of the
Clostridium subphylum.
Supplementary Fig. S2. Maximum-parsimony phylogenetic
tree based on 16S rRNA gene sequences of strain CCRI-16110
T(
R. gauvreauii sp. nov.) and related taxa of
Gram-positive cocci within clusters XIVa (
Clostridium coccoides group) and IV (
Clostridium leptum group).
[PDF file of Supplementary
Figs S1 and S2](792 KB)