1887

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Volume 57, Issue 12

Proposal of gen. nov. and reclassification of , and as gen. nov., comb. nov., comb. nov. and comb. nov. Free

Abstract

A polyphasic study was undertaken to clarify the taxonomic position of endospore-forming strains 433-D9, 433-E17 and 121-X1. BOX-PCR-generated fingerprints indicated that they may be members of a single species. 16S rRNA gene sequence similarity demonstrated that a representative of this group, 433-D9, is affiliated closely with DSM 16317 (100 %), DSM 16319 (99.8 %) and NRRL BD-87 (97.1 %). Sequence similarities revealed NRRL NRS-1691 and several species as the next nearest relatives. DNA–DNA hybridization results showed that strain 433-D9 is a member of . Detection of -Lys–-Asp-based peptidoglycan in strain 433-D9, DSM 16317 and DSM 16319 was in agreement with their close relationship, but differentiated these strains from NRRL BD-87 and NRRL NRS-1691, for which -Lys–-Glu was reported. A similar quinone system was detected in strains 433-D9, 433-E17, 121-X1, DSM 16317, DSM 16319 and NRRL BD-87. This system, unusual for bacilli, consisted of the major compound menaquinone MK-8 (69–80 %) and moderate amounts of MK-7 (19–30 %). This observation was in contrast to the predominance of MK-7 of the closest relative NRRL NRS-1691, as also reported for representatives of the closely related non-endospore-forming genus . Strains 433-D9, DSM 16317 and DSM 16319 exhibited homogeneous and discriminative polar lipid profiles and fatty acid profiles consisting of major acids i-C and ai-C and moderate amounts of i-C 10 and i-C I/ai-C B that discriminated them from closely related strains such as NRRL BD-87. On the basis of clear-cut discriminative chemotaxonomic markers, we propose strains 433-D9, 433-E17 and 121-X1, DSM 16317, DSM 16319 and NRRL BD-87 to be reclassified within a separate genus. For this new taxon, we propose the name gen. nov., and we propose the reclassification of , and as gen. nov., comb. nov. (the type species of , with the type strain DSM 16317 =LMG 22165), comb. nov. (type strain DSM 16319 =LMG 22166) and comb. nov. (type strain NRRL BD-87 =DSM 15031 =JCM 11077).

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2007-12-01
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Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov.
Int J Syst Evol Microbiol 57, 2729 (2007); https://doi.org/10.1099/ijs.0.65256-0
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