A Gram-negative, aerobic, light-orange-coloured, marine bacterium (designated SST-19T) was isolated from beach sediment in Jeju, Korea, and its taxonomic position was determined by means of a polyphasic approach. In a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain SST-19T was positioned within the family ‘Saprospiraceae’, class Sphingobacteria, and formed a consistent cluster with Lewinella persica ATCC 23167T (94.7 % sequence similarity). The levels of 16S rRNA gene sequence similarity between the isolate and recognized members of the family ranged from 84 to 89 %, with Lewinella cohaerens ATCC 23123T, Lewinella nigricans ATCC 23147T, Haliscomenobacter hydrossis DSM 1100T and Saprospira grandis ATCC 23119T showing values of 89.0 , 88.4 , 86.4 and 84.1 %, respectively. Strain SST-19T required seawater for growth and its cells were unbranched, flexible rods or filaments with gliding motility. The cellular fatty acids consisted mainly of saturated, unsaturated and iso-branched fatty acids, the major components being C16 : 1ω7c and/or iso-C15 : 0 2-OH (24.1 %), C18 : 0 (13.5 %), iso-C15 : 0 (11.1 %) and C16 : 0 (10.9 %). The G+C content of the DNA was found to be 51.3 mol%. On the basis of the results from phenotypic and phylogenetic analyses, the isolate represents a novel species of the genus Lewinella, for which the name Lewinella agarilytica sp. nov. is proposed. The type strain is SST-19T (=JBRI 2009T=KCTC 12774T=JCM 14216T).
BernardetJ.-F.,
NakagawaY.,
HolmesB.2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070[CrossRef]
BrosiusJ.,
PalmerJ. L.,
KennedyJ. P.,
NollerH. F.1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli
. Proc Natl Acad Sci U S A 75:4801–4805[CrossRef]
GarrityG. M.,
HoltJ. G.2001; Phylum BVI. Chloroflexi phy. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn., vol. 1 (The Archaea and the deeply branching and phototrophic Bacteria ) pp 427–446 Edited by
BooneD. R.,
CastenholzR. W.,
GarrityG. M.
New York: Springer;
GordonR. E.,
BarnettD. A.,
HanderhanJ. E.,
PangC. H.-N.1974; Nocardia coeliaca, Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63[CrossRef]
HoltJ. G.1989; Genus Herpetosiphon Holt and Lewin, 1965, 2408AL
. In Bergey's Manual of Systematic Bacteriology vol 3 pp 2136–2138 Edited by
StaleyJ. T.,
BryantM. P.,
PfennigN.,
HoltJ. G.
Baltimore: Williams & Wilkins;
JukesT. H.,
CantorC. R.1969; Evolution of protein molecules. In Mammalian Protein Metabolismvol. 3 pp 21–132 Edited by
MunroH. N.
New York: Academic Press;
LeeS. D.2006a; Blastococcus jejuensis sp. nov., an actinomycete from beach sediment, and emended description of the genus Blastococcus Ahrens and Moll 1970. Int J Syst Evol Microbiol 56:2391–2396[CrossRef]
LeeS. D.2006c; Kineococcus marinus sp. nov., isolated from marine sediment of the coast of Jeju, Korea. Int J Syst Evol Microbiol 56:1279–1283[CrossRef]
LeeS. D.2007; Tamnana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae , isolated from beach sediment in Korea. Int J Syst Evol Microbiol 57:764–769[CrossRef]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
SlyL. I.,
TaghaviM.,
FeganM.1998; Phylogenetic heterogeneity within the genus Herpetosiphon : transfer of the marine species Herpetosiphon cohaerans , Herpetosiphon nigricans and Herpetosiphon persicus to the genus Lewinella gen. nov. in the Flexibacter – Bacteroides – Cytophaga phylum.. Int J Syst Bacteriol 48:731–737[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
van VeenW. L.,
van der KooijD.,
GeuzeE. C. W. A.,
van der VliesA. W.1973; Investigations on the sheathed bacterium Haliscomenobacter hydrossis gen. n., sp. n., isolated from activated sludge. Antonie Van Leeuwenhoek 39:207–216[CrossRef]