1887

Abstract

A Gram-negative, aerobic, light-orange-coloured, marine bacterium (designated SST-19) was isolated from beach sediment in Jeju, Korea, and its taxonomic position was determined by means of a polyphasic approach. In a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain SST-19 was positioned within the family ‘’, class , and formed a consistent cluster with ATCC 23167 (94.7 % sequence similarity). The levels of 16S rRNA gene sequence similarity between the isolate and recognized members of the family ranged from 84 to 89 %, with ATCC 23123, ATCC 23147, DSM 1100 and ATCC 23119 showing values of 89.0 , 88.4 , 86.4 and 84.1 %, respectively. Strain SST-19 required seawater for growth and its cells were unbranched, flexible rods or filaments with gliding motility. The cellular fatty acids consisted mainly of saturated, unsaturated and iso-branched fatty acids, the major components being C 7 and/or iso-C 2-OH (24.1 %), C (13.5 %), iso-C (11.1 %) and C (10.9 %). The G+C content of the DNA was found to be 51.3 mol%. On the basis of the results from phenotypic and phylogenetic analyses, the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SST-19 (=JBRI 2009=KCTC 12774=JCM 14216).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65254-0
2007-12-01
2020-09-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/12/2814.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65254-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  2. Brosius J., Palmer J. L., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Garrity G. M., Holt J. G. 2001; Phylum BVI. Chloroflexi phy. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn., vol. 1 (The Archaea and the deeply branching and phototrophic Bacteria ) pp 427–446 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  5. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  6. Gross J. 1911; über freilebende Spironemaceen. Mitt Zool Stat Neapel 20:188–203
    [Google Scholar]
  7. Holt J. G. 1989; Genus Herpetosiphon Holt and Lewin, 1965, 2408AL . In Bergey's Manual of Systematic Bacteriology vol 3 pp 2136–2138 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. Holt J. G., Lewin R. A. 1968; Herpetosiphon aurantiacus gen. et sp. n., a new filamentous gliding organism. J Bacteriol 95:2407–2408
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  10. Lee S. D. 2006a; Blastococcus jejuensis sp. nov., an actinomycete from beach sediment, and emended description of the genus Blastococcus Ahrens and Moll 1970. Int J Syst Evol Microbiol 56:2391–2396 [CrossRef]
    [Google Scholar]
  11. Lee S. D. 2006b; Brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment. Int J Syst Evol Microbiol 56:1889–1892 [CrossRef]
    [Google Scholar]
  12. Lee S. D. 2006c; Kineococcus marinus sp. nov., isolated from marine sediment of the coast of Jeju, Korea. Int J Syst Evol Microbiol 56:1279–1283 [CrossRef]
    [Google Scholar]
  13. Lee S. D. 2007; Tamnana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae , isolated from beach sediment in Korea. Int J Syst Evol Microbiol 57:764–769 [CrossRef]
    [Google Scholar]
  14. Lewin R. A. 1970; New Herpetosiphon species (Flexibacterales). Can J Microbiol 16:517–520 [CrossRef]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  18. Sly L. I., Taghavi M., Fegan M. 1998; Phylogenetic heterogeneity within the genus Herpetosiphon : transfer of the marine species Herpetosiphon cohaerans , Herpetosiphon nigricans and Herpetosiphon persicus to the genus Lewinella gen. nov. in the Flexibacter Bacteroides Cytophaga phylum.. Int J Syst Bacteriol 48:731–737 [CrossRef]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  20. van Veen W. L., van der Kooij D., Geuze E. C. W. A., van der Vlies A. W. 1973; Investigations on the sheathed bacterium Haliscomenobacter hydrossis gen. n., sp. n., isolated from activated sludge. Antonie Van Leeuwenhoek 39:207–216 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65254-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65254-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error