RT Journal Article SR Electronic(1) A1 Lu, Shipeng A1 Lee, Jung Ro A1 Ryu, Seung Hyun A1 Chung, Bok Sil A1 Choe, Woo-Seok A1 Jeon, Che OkYR 2007 T1 Runella defluvii sp. nov., isolated from a domestic wastewater treatment plant JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 11 SP 2600 OP 2603 DO https://doi.org/10.1099/ijs.0.65252-0 PB Microbiology Society, SN 1466-5034, AB A long, Gram-negative, rod-shaped bacterium, designated strain EMB13T, was isolated from a wastewater treatment plant in Korea. The isolate was strictly aerobic and non-motile. The strain grew optimally at 30–35 °C and pH 7.5–8.0, and the predominant fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), C16 : 1 ω5c and iso-C17 : 0 3-OH. The strain contained a large amount of phosphatidylethanolamine and small amounts of phosphatidylcholine and an unknown phospholipid as the polar lipids. The G+C content of the genomic DNA was 40.1 mol% and the major isoprenoid quinone was menaquinone-7. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB13T belonged to the genus Runella and was most closely related to Runella limosa EMB111T, with a 16S rRNA gene sequence similarity of 97.1 %. DNA–DNA relatedness between strain EMB13T and R. limosa EMB111T was approximately 25 %. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain EMB13T represents a novel species within the genus Runella, for which the name Runella defluvii sp. nov. is proposed. The type strain is EMB13T (=KCTC 12614T =DSM 17976T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65252-0