1887

Abstract

A long, Gram-negative, rod-shaped bacterium, designated strain EMB13, was isolated from a wastewater treatment plant in Korea. The isolate was strictly aerobic and non-motile. The strain grew optimally at 30–35 °C and pH 7.5–8.0, and the predominant fatty acids were iso-C, summed feature 3 (C 7 and/or iso-C 2-OH), C 5 and iso-C 3-OH. The strain contained a large amount of phosphatidylethanolamine and small amounts of phosphatidylcholine and an unknown phospholipid as the polar lipids. The G+C content of the genomic DNA was 40.1 mol% and the major isoprenoid quinone was menaquinone-7. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB13 belonged to the genus and was most closely related to EMB111, with a 16S rRNA gene sequence similarity of 97.1 %. DNA–DNA relatedness between strain EMB13 and EMB111 was approximately 25 %. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain EMB13 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is EMB13 (=KCTC 12614 =DSM 17976).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65252-0
2007-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2600.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65252-0&mimeType=html&fmt=ahah

References

  1. Chelius M. K., Triplett E. W. 2000; Dyadobacter fermentans gen. nov., sp. nov., a novel Gram-negative bacterium isolated from surface-sterilized Zea mays stems. Int J Syst Evol Microbiol 50:751–758 [CrossRef]
    [Google Scholar]
  2. Chelius M. K., Henn J. A., Triplett E. W. 2002; Runella zeae sp. nov., a novel Gram-negative bacterium from the stems of surface-sterilized Zea mays . Int J Syst Evol Microbiol 52:2061–2063 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 2002 phylip (phylogeny inference package) version 3.6a. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  5. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146
    [Google Scholar]
  6. Jeon C. O., Lim J.-M., Lee J.-M., Xu L.-H., Jiang C.-L., Kim C.-J. 2005; Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55:1891–1896 [CrossRef]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  9. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  10. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  11. Larkin J. M., Williams P. M. 1978; Runella slithyformis gen. nov., sp. nov., a curved, nonflexible, pink bacterium. Int J Syst Bacteriol 28:32–36 [CrossRef]
    [Google Scholar]
  12. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  13. Lu S., Park M., Ro H.-S., Lee D. S., Park W., Jeon C. O. 2006; Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. J Microbiol 44:155–161
    [Google Scholar]
  14. Park M., Lu S., Ryu S. H., Chung B. S., Park W., Kim C. J., Jeon C. O. 2006; Flavobacterium croceum sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 56:2443–2447 [CrossRef]
    [Google Scholar]
  15. Park M., Ryu S. H., Vu T. H., Ro H. S., Yun P. Y., Jeon C. O. 2007; Flavobacterium defluvii sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 57:233–237 [CrossRef]
    [Google Scholar]
  16. Reddy G. S. N., Garcia-Pichel F. 2005; Dyadobacter crusticola sp. nov., from biological soil crusts in the Colorado Plateau, USA, and an emended description of the genus Dyadobacter Chelius and Triplett 2000. Int J Syst Evol Microbiol 55:1295–1299 [CrossRef]
    [Google Scholar]
  17. Ryu S. H., Nguyen M. T., Park W., Kim C.-J., Jeon C. O. 2006; Runella limosa sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 56:2757–2760 [CrossRef]
    [Google Scholar]
  18. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  19. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C., Nesme X., Rosselló-Mora R., Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  20. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65252-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65252-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error