1887

Abstract

Two strains of lactic acid bacteria were isolated from faeces of two actively racing thoroughbred horses. The isolates formed a subcluster in the phylogenetic group, closely related to , , and , by phylogenetic analysis based on 16S rRNA gene sequences. Levels of DNA–DNA relatedness revealed that the isolates belonged to the same taxon and were genetically separated from their phylogenetic relatives. Biochemical and physiological characteristics also distinguished the isolates from their phylogenetic relatives. The isolates produced spherical or oval cells, and tetrad-like cells were rarely seen. To the best of our knowledge, this is the first report of this morphological characteristic within the genus . Thus, the isolates represent an atypical novel species of the genus , for which the name sp. nov. is proposed. The type strain is NRIC 0697 (=JCM 14505 =DSM 18793).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65250-0
2008-04-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/4/914.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65250-0&mimeType=html&fmt=ahah

References

  1. Baele, M., Vancanneyt, M., Devriese, L. A., Lefebvre, K., Swings, J. & Haesebrouck, F. ( 2003; ). Lactobacillus ingluviei sp. nov., isolated from the intestinal tract of pigeons. Int J Syst Evol Microbiol 53, 133–136.[CrossRef]
    [Google Scholar]
  2. Brashears, M. M., Jaroni, D. & Trimble, J. ( 2003; ). Isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of Escherichia coli O157 : H7 in cattle. J Food Prot 66, 355–363.
    [Google Scholar]
  3. Casey, P. G., Casey, G. D., Gardiner, G. E., Tangney, M., Stanton, C., Ross, R. P., Hill, C. & Fitzgerald, G. F. ( 2004; ). Isolation and characterization of anti-Salmonella lactic acid bacteria from the porcine gastrointestinal tract. Lett Appl Microbiol 39, 431–438.[CrossRef]
    [Google Scholar]
  4. Cavalli-Sforza, L. L. & Edwards, A. W. F. ( 1967; ). Phylogenetic analysis models and estimation procedures. Am J Hum Genet 19, 233–257.
    [Google Scholar]
  5. Collins, M. D., Rodrigues, U. M., Ash, C., Aguirre, M., Farrow, J. A. E., Martinez-Murcia, A., Philips, B. A., Williams, A. M. & Wallbanks, S. ( 1991; ). Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA. FEMS Microbiol Lett 77, 5–12.[CrossRef]
    [Google Scholar]
  6. Collins, M. D., Samelis, J., Metaxopoulos, J. & Wallbanks, S. ( 1993; ). Taxonomic studies on some leuconostoc-like organisms from fermented sausages: description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. J Appl Microbiol 75, 595–603.
    [Google Scholar]
  7. Dellaglio, F., Torriani, S. & Felis, G. E. ( 2004; ). Reclassification of Lactobacillus cellobiosus Rogosa et al. 1953 as a later synonym of Lactobacillus fermentum Beijerinck 1901. Int J Syst Evol Microbiol 54, 809–812.[CrossRef]
    [Google Scholar]
  8. Endo, A. & Okada, S. ( 2005; ). Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials. Int J Syst Evol Microbiol 55, 83–85.[CrossRef]
    [Google Scholar]
  9. Endo, A. & Okada, S. ( 2006; ). Oenococcus kitaharae sp. nov., a novel non-acidophilic and non-malolactic fermenting oenococcus isolated from a composting distilled shochu residue. Int J Syst Evol Microbiol 56, 2345–2348.[CrossRef]
    [Google Scholar]
  10. Endo, A., Okada, S. & Morita, H. ( 2007; ). Molecular profiling of Lactobacillus, Streptococcus, and Bifidobacterium species in feces of active racehorses. J Gen Appl Microbiol 53, 191–200.[CrossRef]
    [Google Scholar]
  11. Ezaki, T., Yamamoto, N., Ninomiya, K., Suzuki, S. & Yabuuchi, E. ( 1983; ). Transfer of Peptococcus indolicus, Peptococcus asaccharolyticus, Peptococcus prevotii, and Peptococcus magnus to the genus Peptostreptococcus and proposal of Peptostreptococcus tetradius sp. nov. Int J Syst Bacteriol 33, 683–698.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  13. Garvie, E. I. ( 1986; ). Genus Pediococcus Claussen 1903. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1075–1079. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  14. Hammes, W. P. & Hertel, C. ( 2006; ). The genera Lactobacillus and Carnobacterium. In The Prokaryotes, 3rd edn, vol. 4, pp. 320–403. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  15. Hammes, W. P. & Vogel, R. F. ( 1996; ). The genus Lactobacillus. In The Lactic Acid Bacteria, vol. 2, The Genera of Lactic Acid Bacteria, pp. 19–54. Edited by B. J. B. Wood & W. H. Holzapfel. London: Blackie.
  16. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  17. Kluge, A. G. & Farris, F. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  18. Liu, L., Zhang, B., Tong, H. & Dong, X. ( 2006; ). Pediococcus ethanolidurans sp. nov., isolated from the walls of a distilled-spirit-fermenting cellar. Int J Syst Evol Microbiol 56, 2405–2408.[CrossRef]
    [Google Scholar]
  19. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  20. Morita, H., Shiratori, M., Murakami, M., Takami, H., Kato, Y., Endo, A., Nakajima, F., Takagi, M., Akita, H. & other authors ( 2007; ). Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds. Int J Syst Evol Microbiol 57, 2836–2839.[CrossRef]
    [Google Scholar]
  21. Ouwehand, A. C., Salminen, S. & Isolauri, E. ( 2002; ). Probiotics: an overview of beneficial effects. Antonie van Leeuwenhoek 82, 279–289.[CrossRef]
    [Google Scholar]
  22. Rogosa, M. ( 1974; ). Genus Lactobacillus. In Bergey's Manual of Determinative Bacteriology, 8th edn, pp. 576–593. Edited by R. E. Buchanan & N. E. Gibbons. Baltimore: Williams & Wilkins.
  23. Roos, S., Karner, F., Axelsson, L. & Jonsson, H. ( 2000; ). Lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine. Int J Syst Evol Microbiol 50, 251–258.[CrossRef]
    [Google Scholar]
  24. Roos, S., Engstrand, L. & Jonsson, H. ( 2005; ). Lactobacillus gastricus sp. nov., Lactobacillus antri sp. nov., Lactobacillus kalixensis sp. nov. and Lactobacillus ultunensis sp. nov., isolated from human stomach mucosa. Int J Syst Evol Microbiol 55, 77–82.[CrossRef]
    [Google Scholar]
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  26. Schleifer, K. H. & Ludwig, W. ( 1995; ). Phylogeny of the genus Lactobacillus and related genera. Syst Appl Microbiol 18, 461–467.[CrossRef]
    [Google Scholar]
  27. Simpson, W. J. & Taguchi, H. ( 1996; ). The genus Pediococcus with notes on the genera Tetragenococcus and Aerococcus. In The Lactic Acid Bacteria, vol. 2, The Genera of Lactic Acid Bacteria, pp. 125–172. Edited by B. J. B. Wood & W. H. Holzapfel. London: Blackie.
  28. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  29. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  30. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  31. Tong, H. & Dong, X. ( 2005; ). Lactobacillus concavus sp. nov., isolated from the walls of a distilled spirit fermenting cellar in China. Int J Syst Evol Microbiol 55, 2199–2202.[CrossRef]
    [Google Scholar]
  32. Walter, J., Hertel, C., Tannock, G. W., Lis, C. M., Munro, K. & Hammes, W. P. ( 2001; ). Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human faeces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol 67, 2578–2585.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65250-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65250-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 914 - 918

Phylogenetic relationships of strains NRIC 0696 and NRIC 0697 ( sp. nov.) to related species based on 16S rRNA gene sequences, based on the maximum-likelihood method (Fig. S1) and the maximum-parsimony method (Fig. S2). [PDF](22 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error