sp. nov., isolated from Lake Ejinor, Inner Mongolia, China Free

Abstract

A novel halophilic archaeon, strain EJ-32, was isolated from water from Lake Ejinor in Inner Mongolia, China. The taxonomy of strain EJ-32 was studied by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain EJ-32 was shown to be phylogenetically related to (97.9 %), (97.9 %), (97.8 %), (97.8 %), (97.6 %), (97.4 %), (97.1 %) and (96.4 %). Strain EJ-32 was found to be neutrophilic, non-motile and Gram-negative. It grew in medium containing saturation concentrations of NaCl and did not require magnesium for optimal growth. The G+C content of the DNA is 64.0 mol%. Values for DNA–DNA hybridization with respect to phylogenetically related species were ≤49 %, indicating that EJ-32 constitutes a different genospecies. The data show that strain EJ-32 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EJ-32 (=CECT 7194=CGMCC 1.6782=JCM 14265).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65241-0
2007-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2538.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65241-0&mimeType=html&fmt=ahah

References

  1. Arahal D. R., Dewhirst F. E., Paster B. J., Volcani B. E., Ventosa A. 1996; Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Appl Environ Microbiol 62:3779–3786
    [Google Scholar]
  2. Castillo A. M., Gutiérrez M. C., Kamekura M., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. 2006; Halorubrum orientale sp. nov., a halophilic archaeon isolated from Lake Ejinor, Inner Mongolia, China. Int J Syst Evol Microbiol 56:2559–2563 [CrossRef]
    [Google Scholar]
  3. Cui H.-L., Tohty D., Zhou P.-J., Liu S.-J. 2006; Halorubrum lipolyticum sp. nov. and Halorubrum aidingense sp. nov., isolated from two salt lakes in Xin-Jiang, China. Int J Syst Evol Microbiol 56:1631–1634 [CrossRef]
    [Google Scholar]
  4. Fan H., Xue Y., Ma Y., Ventosa A., Grant W. D. 2004; Halorubrum tibetense sp. nov., a novel haloalkaliphilic archaeon from Lake Zabuye in Tibet, China. Int J Syst Evol Microbiol 54:1213–1216 [CrossRef]
    [Google Scholar]
  5. Feng J., Zhou P., Liu S. 2004; Halorubrum xinjiangense sp. nov., a novel halophile isolated from saline lakes in China. Int J Syst Evol Microbiol 54:1789–1791 [CrossRef]
    [Google Scholar]
  6. Feng J., Zhou P., Zhou Y., Liu S., Warren-Rhodes K. 2005; Halorubrum alkaliphilum sp. nov., a novel haloalkaliphile isolated from a soda lake in Xinjiang, China. Int J Syst Evol Microbiol 55:149–152 [CrossRef]
    [Google Scholar]
  7. Fitch W. M. 1971; Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. Franzmann P. D., Stackebrandt E., Sanderson K., Volkman J. K., Cameron D. E., Stevenson P. L., McMeekin T. A., Burton H. R. 1988; Halobacterium lacusprofundi sp. nov., a halophilic bacterium isolated from Deep Lake, Antarctica. Syst Appl Microbiol 11:20–27 [CrossRef]
    [Google Scholar]
  9. Grant W. D., Kamekura M., McGenity T. J., Ventosa A. 2001; Order I. Halobacteriales Grant & Larsen 1989b, 495VP (Effective publication: Grant & Larsen 1989a, 2216. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp 294–334 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  10. Gutiérrez M. C., Kamekura M., Holmes M. L., Dyall-Smith M. L., Ventosa A. 2002; Taxonomic characterization of Haloferax sp. (“H. alicantei ”) strain Aa 2.2: description of Haloferax lucentesis sp. nov.. Extremophiles 6479–483 [CrossRef]
    [Google Scholar]
  11. Gutiérrez M. C., Castillo A. M., Kamekura M., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. 2007; Halopiger xanaduensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from saline Lake Shangmatala in Inner Mongolia, China. Int J Syst Evol Microbiol 571402–1407 [CrossRef]
    [Google Scholar]
  12. Johnson J. L. 1994; Similarity analysis of DNAs. In Methods for General and Molecular Biotechnology . pp 655–681 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  13. Kamekura M. 1993; Lipids of extreme halophiles. In The Biology of Halophilic Bacteria . pp 135–161 Edited by Vreeland R. H., Hochstein L. I. Boca Raton: CRC Press;
  14. Kamekura M., Dyall-Smith M. L. 1995; Taxonomy of the family Halobacteriaceae and the description of two genera Halorubrobacterium and Natrialba . J Gen Appl Microbiol 41:333–350 [CrossRef]
    [Google Scholar]
  15. Kamekura M., Dyall-Smith M. L., Upasani V., Ventosa A., Kates M. 1997; Diversity of alkaliphilic halobacteria: proposals for transfer of Natronobacterium vacuolatum, Natronobacterium magadii, and Natronobacterium pharaonis to Halorubrum, Natrialba and Natronomonas gen.nov., respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis comb. nov., respectively. Int J Syst Bacteriol 47:853–857 [CrossRef]
    [Google Scholar]
  16. Kharroub K., Quesada E., Ferrer R., Fuentes S., Aguilera M., Boulahrouf A., Ramos-Cormenzana A., Monteoliva-Sánchez M. 2006; Halorubrum ezzemoulense sp. nov., a halophilic archaeon isolated from Ezzemoul sabkha, Algeria. Int J Syst Evol Microbiol 56:1583–1588 [CrossRef]
    [Google Scholar]
  17. Lizama C., Monteoliva-Sanchez M., Suarez-Garcia A., Rosello-Mora R., Aguilera M., Campos V., Ramos-Cormenzana A. 2002; Halorubrum tebenquichense sp. nov., a novel halophilic archaeon isolated from the Atacama Saltern, Chile. Int J Syst Evol Microbiol 52:149–155
    [Google Scholar]
  18. Lopez-Garcia P., Moreira D., Lopez-Lopez A., Rodríguez-Valera F. 2001; A novel haloarchaeal-related lineage is widely distributed in deep oceanic regions. Environ Microbiol 3:72–78 [CrossRef]
    [Google Scholar]
  19. Ludwig W., Strunk O., Klugbauer S., Klugbauer N., Weizenernegger M., Neumaier J., Bachleitner M., Schleifer K.-H. 1998; Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19:554–568 [CrossRef]
    [Google Scholar]
  20. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  21. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  22. McGenity T. J., Grant W. D. 1995; Transfer of Halobacterium saccharovorum, Halobacterium sodomense, Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the genus Halorubrum gen. nov., as Halorubrum saccharovorum comb nov., Halorubrum sodomense comb nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov. Syst Appl Microbiol 18:237–243 [CrossRef]
    [Google Scholar]
  23. McGenity T. J., Grant W. D. 2001; Genus VII. Halorubrum . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp 320–324 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  24. Mwatha W. E., Grant W. D. 1993; Natronobacterium vacuolata sp. nov., a haloalkaliphilic archaeon isolated from Lake Magadi, Kenya. Int J Syst Bacteriol 43:401–404 [CrossRef]
    [Google Scholar]
  25. Oren A. 1983; Halorubrum sodomense sp. nov., a Dead Sea halobacterium with an extremely high magnesium requirement. Int J Syst Bacteriol 33:381–386 [CrossRef]
    [Google Scholar]
  26. Oren A. 2002 Halophilic Microorganisms and their Environments Dordrecht: Kluwer Academic Publishers;
    [Google Scholar]
  27. Oren A., Ventosa A. 1996; A proposal for the transfer of Halorubrobacterium distributum and Halorubrobacterium coriense to the genus Halorubrum as Halorubrum distributum comb. nov. and Halorubrum coriense comb. nov., respectively. Int J Syst Bacteriol 46:1180 [CrossRef]
    [Google Scholar]
  28. Oren A., Ventosa A., Grant W. D. 1997; Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  29. Owen R. J., Hill L. R. 1979; The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists (Society for Applied Bacteriology Technical Series, no. 14), 2nd edn. pp 277–296 Edited by Skinner F. A., Lovelock D. W. London: Academic Press;
    [Google Scholar]
  30. Petter H. F. M. 1931; On bacteria of salted fish. Proc K Ned Akad Wet Amsterdam 34:1417–1423 (in Dutch
    [Google Scholar]
  31. Rodriguez-Valera F. 1988; Characteristics and microbial ecology of hypersaline environments. In Halophilic Bacteria vol. 1 pp 3–30 Edited by Rodriguez-Valera F. Boca Raton: CRC Press;
    [Google Scholar]
  32. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  33. Tomlinson G. A., Hochstein L. I. 1976; Halobacterium saccharovorum sp. nov., a carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 22:587–591 [CrossRef]
    [Google Scholar]
  34. Ventosa A. 2006; Unusual micro-organisms from unusual habitats: hypersaline environments. In Prokaryotic Diversity: Mechanisms and Significance . pp 223–253 Edited by Logan N. A., Lappin-Scott H. M., Oyston P. C. F. Cambridge: Cambridge University Press;
  35. Ventosa A., Gutiérrez M. C., Kamekura M., Zvyagintseva I. S., Oren A. 2004; Taxonomic study of Halorubrum distributum and proposal of Halorubrum terrestre sp. nov. Int J Syst Evol Microbiol 54:389–392 [CrossRef]
    [Google Scholar]
  36. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  37. Zvyagintseva I. S., Tarasov A. L. 1987; Extreme halophilic bacteria from saline soils. Microbiology (English translation of Mikrobiologiya) 56:839–844 (in Russian)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65241-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65241-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed