gen. nov., sp. nov., a long-chain n-alkene-degrading, sulfate-reducing bacterium Free

Abstract

A novel anaerobic, long-chain alkene-degrading, sulfate-reducing bacterium, strain LM2801, was isolated from brackish sediment of a wastewater decantation facility of an oil refinery (Berre lagoon, France). Cells of strain LM2801 were Gram-negative, motile, slightly curved or vibrioid rods. Its optimum growth conditions were 30–36 °C, 6–10 g NaCl l and pH 7.5. Strain LM2801 incompletely oxidized long-chain alkenes (from C to C) and fatty acids (C to C). The DNA G+C content was 45.5 mol%. Sequence analyses of the 16S rRNA and genes indicated that the strain was a member of the family within the . This novel isolate possesses phenotypic and phylogenetic traits that do not allow its classification as a member of any previously described genus. Therefore, strain LM2801 is described as a member of a new genus, gen. nov., of which sp. nov. is the type species. The type strain of is LM2801 (=DSM 18843 =JCM 14469).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65240-0
2007-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2699.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65240-0&mimeType=html&fmt=ahah

References

  1. Aeckersberg F., Bak F., Widdel F. 1991; Anaerobic oxidation of saturated hydrocarbons to CO2 by a new type of sulfate-reducing bacterium. Arch Microbiol 156:5–14 [CrossRef]
    [Google Scholar]
  2. Aeckersberg F., Rainey F. A., Widdel F. 1998; Growth, natural relationships, cellular fatty acids and metabolic adaptation of sulfate-reducing bacteria that utilize long-chain alkanes under anoxic conditions. Arch Microbiol 170:361–369 [CrossRef]
    [Google Scholar]
  3. Buck J. D. 1982; Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993
    [Google Scholar]
  4. Cline J. D. 1969; Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 14:454–458 [CrossRef]
    [Google Scholar]
  5. Cravo-Laureau C., Matheron R., Cayol J.-L., Joulian C., Hirschler-Réa A. 2004a; Desulfatibacillum aliphaticivorans gen. nov., sp. nov. an n-alkane- and n-alkene-degrading sulfate-reducing bacterium. Int J Syst Evol Microbiol 54:77–83 [CrossRef]
    [Google Scholar]
  6. Cravo-Laureau C., Matheron R., Joulian C., Cayol J.-L., Hirschler-Réa A. 2004b; Desulfatibacillum alkenivorans sp. nov., a novel n-alkene-degrading, sulfate-reducing bacterium, and emended description of the genus Desulfatibacillum . Int J Syst Evol Microbiol 54:1639–1642 [CrossRef]
    [Google Scholar]
  7. Curiale J. A., Frolov E. B. 1998; Occurrence and origin of olefins in crude oils. A critical review. Org Geochem 29:397–408 [CrossRef]
    [Google Scholar]
  8. Kolmert A., Wikström P., Hallberg K. B. 2000; A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures. J Microbiol Methods 41:179–184 [CrossRef]
    [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  10. So C. M., Young L. Y. 1999; Isolation and characterization of a sulfate-reducing bacterium that anaerobically degrades alkanes. Appl Environ Microbiol 65:2969–2976
    [Google Scholar]
  11. Vogel A. I. 1961 A Text-Book of Quantitative Inorganic Analysis , 3rd edn. London: Longmans;
    [Google Scholar]
  12. Widdel F., Bak F. 1992; Gram-negative mesophilic sulfate-reducing bacteria. In The Prokaryotes , 2nd edn. vol 4 pp 3352–3378 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  13. Widdel F., Musat F., Knittel K., Galushko A. 2007; Anaerobic degradation of hydrocarbons with sulphate as electron acceptor. In Sulfate-Reducing Bacteria: Environmental and Engineered Systems pp 265–303 Edited by Barton L. L., Hamilton W. A. Cambridge: Cambridge University Press;
    [Google Scholar]
  14. Wind T., Stubner S., Conrad R. 1999; Sulfate-reducing bacteria in rice field soil and on rice roots. Syst Appl Microbiol 22:269–279 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65240-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65240-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed