Five novel strains (2002, 2902, 2006, 108 and 117) of cellulose-degrading, anaerobic, thermophilic bacteria were isolated from terrestrial hot springs of Kamchatka (Far East, Russia). Strains 2002 and 108 were non-spore-forming bacteria with a Gram-positive type cell wall and peritrichous flagella. Optimum growth of strains 2002 and 108 occurred at pH 7.0 and at temperatures of 70 and 65 °C, respectively. The G+C contents of the DNA of strains 2002 and 108 were 35.1 and 36.4 mol%, respectively. Comparative 16S rRNA gene sequence analysis revealed that the isolates belonged to the genus . However, DNA–DNA hybridization experiments indicated that the levels of relatedness between strains 2002 and 108 and those of recognized members of the genus ranged between 32 and 54 %. Based on both phenotypic and genomic differences, strains 2002 and 108 are considered to represent two novel species of the genus . The names proposed for these organisms are sp. nov. (type strain 2002=DSM 18902=VKM B-2412) and sp. nov. (type strain 108=DSM 18901=VKM B-2411).


Article metrics loading...

Loading full text...

Full text loading...



  1. Bergquist, P. L., Saul, D. J., Gibbs, M. D., Morris, D. D., Te'o, V. S. J. & Morgan, H. W.(1999). Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria. FEMS Microbiol Ecol 28, 99–110.[CrossRef] [Google Scholar]
  2. Bonch-Osmolovskaya, E. A., Sokolova, T. G., Kostrikina, N. A. & Zavarzin, G. A.(1990).Desulfurella acetivorans gen. nov. and sp. nov. – a new thermophilic sulfur-reducing eubacterium. Arch Microbiol 153, 151–155.[CrossRef] [Google Scholar]
  3. Bredholt, S., Sonne-Hansen, J., Nielsen, P., Mathrani, I. M. & Ahring, K.(1999).Caldicellulosiruptor kristjanssonii, sp. nov., a cellulolytic, extremely thermophilic, anaerobic bacterium. Int J Syst Bacteriol 49, 991–996.[CrossRef] [Google Scholar]
  4. Garrity, G. M., Boone, D. R. & Castenholz, R. W. (editors)(2001).Bergey's Manual of Systematic Bacteriology, vol. 1, 2nd edn. New York: Springer.
  5. Huang, C.-Y., Patel, B. K., Mah, R. A. & Baresi, L.(1998).Caldicellulosiruptor owensensis sp. nov., an anaerobic, extremely thermophilic, xylanolytic bacterium. Int J Syst Bacteriol 48, 91–97.[CrossRef] [Google Scholar]
  6. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  7. Miroshnichenko, M. L., Gongadze, G. A., Lysenko, A. M. & Bonch-Osmolovskaya, E. A.(1994).Desulfurella multipotens sp. nov., a new sulfur-respiring thermophilic eubacterium from Raoul Island (Kermadec archipelago, New Zealand). Arch Microbiol 161, 88–93. [Google Scholar]
  8. Mladenovska, Z., Mathrani, I. M. & Ahring, B. K.(1995). Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium. Arch Microbiol 163, 223–230.[CrossRef] [Google Scholar]
  9. Onyenwoke, R. U., Lee, Y.-J., Dabrowski, S., Ahring, B. K. & Wiegel, J.(2006). Reclassification of Thermoanaerobium acetigenum as Caldicellulosiruptoracetigenus comb. nov. and emendation of the genus description. Int J Syst Evol Microbiol 56, 1391–1395.[CrossRef] [Google Scholar]
  10. Pfennig, N.(1965). Anreicherungskulturen für rote und grüne Schwefelbakterien. Zentralbl Bakteriol I Abt IS (Suppl. V), 179–185. [Google Scholar]
  11. Pfennig, N. & Lippert, K. D.(1966). Über das Vitamin B12-Bedürfnis phototropher Schwefelbacterien. Arch Microbiol 55, 245–256. [Google Scholar]
  12. Rainey, F. A., Donnison, A. M., Janssen, P. H., Saul, D., Rodrigo, A., Berquist, P. L., Daniel, R. M., Stackebrandt, E. & Morgan, H. W.(1994). Description of Caldicellulosiruptor saccharolyticus gen. nov., sp. nov.: an obligately anaerobic, extremely thermophilic, cellulolytic bacterium. FEMS Microbiol Lett 120, 263–266.[CrossRef] [Google Scholar]
  13. Svetlichnii, V. A. & Svetlichnaya, T. P.(1988).Dictyoglomus turgidus sp. nov., a new extreme thermophilic eubacterium isolated from hot springs in the Uzon volcano crater. Microbiology (English translation of Mikrobiologiia) 57, 435–441. [Google Scholar]
  14. Svetlichnii, V. A., Svetlichnaya, T. P., Chernych, N. A. & Zavarzin, G. A.(1990).Anaerocellum thermophilum gen. nov., an extreme thermophilic cellulolytic eubacterium isolated from hot springs in the valley of Geysers. Microbiology (English translation of Mikrobiologiia) 59, 871–879. [Google Scholar]
  15. Van de Peer, Y. & De Wachter, R.(1994).treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570. [Google Scholar]
  16. Wolin, E. A., Wolin, M. J. & Wolfe, R. S.(1963). Formation of methane by bacterial extracts. J Biol Chem 238, 2882–2886. [Google Scholar]

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error