A novel Gram-negative, motile, aerobic rod-shaped bacterium was isolated from a sp. specimen collected from the Logatchev hydrothermal vent field at the Mid-Atlantic Ridge. The novel strain, M41, was catalase- and oxidase-positive and metabolised various carbohydrates and amino acids. It grew well in marine broth with an optimal growth temperature of 31 °C to 34 °C (range 4–40 °C) and salinity requirement of 3 % (range 0.3–9 %). The pH range for growth was pH 4.6 to 9.5, with an optimum at pH 8.0. The predominant fatty acids were C 7, C and C 7. The DNA G+C content of strain M41 was 52.2 mol%. The 16S rRNA gene sequence was 94 % similar to that of the type strain of , the closest cultivated relative. Other related type strains were (93 % gene sequence similarity), (92%) and (92 %). According to phylogenetic analysis and physiological characteristics, it is suggested that strain M41 represents a new genus and novel species for which the name gen. nov., sp. nov. is proposed. The type strain is M41 (=DSM 18887=LMG 24143).


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  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J.(1990). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef] [Google Scholar]
  2. Boyde, A. & Wood, C.(1969). Preparation of animal tissues for surface-scanning electron microscopy. J Microsc 90, 221–249.[CrossRef] [Google Scholar]
  3. Cole, J. R., Chai, B., Farris, R. J., Wang, Q., Kulam-Syed-Mohideen, A. S., McGarrell, D. M., Bandela, A. M., Cardenas, E., Garrity, G. M. & Tiedje, J. M.(2007). The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. Nucleic Acids Res 35, D169–D172.[CrossRef] [Google Scholar]
  4. Faulkner, D. J.(2000). Marine natural products. Nat Prod Rep 17, 7–55.[CrossRef] [Google Scholar]
  5. González, J. & Whitman, W.(2001).Oceanospirillum and related genera. The Prokaryotes, pp. 887–915. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer.
  6. Gregersen, T.(1978). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef] [Google Scholar]
  7. Guindon, S., Lethiec, F., Duroux, P. & Gascuel, O.(2005).phyml online – a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33, W557–W559.[CrossRef] [Google Scholar]
  8. Haefner, B.(2003). Drugs from the deep: marine natural products as drug candidates. Drug Discov Today 8, 536–544.[CrossRef] [Google Scholar]
  9. Hedlund, B. P., Geiselbrecht, A. D., Bair, T. J. & Staley, J. T.(1999). Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov. Appl Environ Microbiol 65, 251–259. [Google Scholar]
  10. Hoek, J., Banta, A., Hubler, F. & Reysenbach, A.-L.(2003). Microbial diversity of a sulphide spire located in the Edmond deep-sea hydrothermal vent field on the Central Indian Ridge. Geobiology 1, 119–127.[CrossRef] [Google Scholar]
  11. Hylemon, P. B., Wells, J. S. J., Krieg, N. R. & Jannasch, H. W.(1973). Genus I Oceanospirillum. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 271–281. Edited by G. M. Garrity, D. J. Brenner, N. R. Krieg & J. T. Staley. New York: Springer.
  12. Jannasch, H. W. & Taylor, C. D.(1984). Deep-sea microbiology. Annu Rev Microbiol 38, 487–514.[CrossRef] [Google Scholar]
  13. Jensen, P. R. & Fenical, W.(1994). Strategies for the discovery of secondary metabolites from marine-bacteria – ecological perspectives. Annu Rev Microbiol 48, 559–584.[CrossRef] [Google Scholar]
  14. Kelecom, A.(2002). Secondary metabolites from marine microorganisms. An Acad Bras Cienc 74, 151–170.[CrossRef] [Google Scholar]
  15. Kelley, D. S., Baross, J. A. & Delaney, J. R.(2002). Volcanoes, fluids, and life at Mid-Ocean Ridge spreading centers. Annu Rev Earth Planet Sci 30, 385–491.[CrossRef] [Google Scholar]
  16. Krieg, N. R.(1976). Biology of the chemoheterotrophic spirilla. Bacteriol Rev 40, 55–115. [Google Scholar]
  17. Lane, D.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  18. Lane, D. J., Pace, B., Olsen, G. J., Stahl, D. A., Sogin, M. L. & Pace, N. R.(1985). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82, 6955–6959.[CrossRef] [Google Scholar]
  19. López-García, P., Duperron, S., Philippot, P., Foriel, J., Susini, J. & Moreira, D.(2003). Bacterial diversity in hydrothermal sediment and epsilonproteobacterial dominance in experimental microcolonizers at the Mid-Atlantic Ridge. Environ Microbiol 5, 961–976.[CrossRef] [Google Scholar]
  20. Ludwig, W.(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  21. Mesbah, M., Premachandran, U. & Whitman, W.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  22. Muyzer, G., Dewaal, E. C. & Uitterlinden, A. G.(1993). Profiling of complex microbial-populations by denaturing gradient gel-electrophoresis analysis of polymerase chain reaction-amplified genes-coding for 16S ribosomal-RNA. Appl Environ Microbiol 59, 695–700. [Google Scholar]
  23. Pearson, W. R.(1990). Rapid and sensitive sequence comparison with fastp and fasta. Methods Enzymol 183, 63–98. [Google Scholar]
  24. Radjasa, O. K., Urakawa, H., Kita-Tsukamoto, K. & Ohwada, K.(2001). Characterization of psychrotrophic bacteria in the surface and deep-sea waters from the northwestern Pacific Ocean based on 16S ribosomal DNA analysis. Mar Biotechnol 3, 454–462.[CrossRef] [Google Scholar]
  25. Reysenbach, A.-L., Longnecker, K. & Kirshtein, J.(2000). Novel bacterial and archaeal lineages from an in situ growth chamber deployed at a Mid-Atlantic Ridge hydrothermal vent. Appl Environ Microbiol 66, 3798–3806.[CrossRef] [Google Scholar]
  26. Rittenberg, S. C. & Williams, M. A.(1956). Microcyst formation and germination in Spirillum lunatum. J Gen Microbiol 15, 205–209.[CrossRef] [Google Scholar]
  27. Romanenko, L. A., Schumann, P., Rohde, M., Mikhailov, V. V. & Stackebrandt, E.(2004).Reinekea marinisedimentorum gen. nov., sp. nov., a novel gammaproteobacterium from marine coastal sediments. Int J Syst Evol Microbiol 54, 669–673.[CrossRef] [Google Scholar]
  28. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  29. Satomi, M., Kimura, B., Hamada, T., Harayama, S. & Fujii, T.(2002). Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov. Int J Syst Evol Microbiol 52, 739–747.[CrossRef] [Google Scholar]
  30. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods of General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.

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