1887

Abstract

Two Gram-negative, rod-shaped, thermophilic bacterial strains, HC145 and HC148, were isolated from a compost sample from a compost facility in Ichon, Korea. Sequencing of the 16S rRNA genes of HC145 and HC148 and comparative analyses of the resulting sequences clearly showed that these strains had a phylogenetic affiliation to the genus . The level of 16S rRNA similarity between the two novel strains was 98.4 % and the levels of sequence similarity between them and existing species were 97.8–98.1 (HC145) and 97.4–98.7 % (HC148). The DNA–DNA reassociation values between the two strains and the type strains of species ranged from 38 to 51 %. The polar lipid profiles for both isolates consisted of phosphatidylglycerol, diphosphatidylglycerol, phospholipids and glycolipids of unknown composition. The major quinones were MK-8, MK-9 and MK-7, the peptidoglycan type was -Lys←-Asp and the main cellular fatty acid was iso-C. The DNA G+C contents of strains HC145 and HC148 were 42.4 and 38.5 mol%, respectively. On the basis of the data from this polyphasic study, strains HC145 and HC148 represent members of the genus , for which the names sp. nov. and sp. nov., respectively, are proposed. The type strain of is HC145 (=KACC 11361 =DSM 17951) and the type strain of is HC148 (=KACC 11362 =DSM 17952).

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2007-12-01
2020-01-17
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