@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65228-0, author = "Roux, Véronique and Fenner, Lukas and Raoult, Didier", title = "Paenibacillus provencensis sp. nov., isolated from human cerebrospinal fluid, and Paenibacillus urinalis sp. nov., isolated from human urine", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "3", pages = "682-687", doi = "https://doi.org/10.1099/ijs.0.65228-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65228-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "CSF, cerebrospinal fluid", abstract = "Gram-negative, spore-forming rods were isolated from a human urine sample (strain 5402403T) and a human cerebrospinal fluid sample (strain 4401170T). Based on genotypic characteristics, these strains belonged to the genus Paenibacillus and were closely related. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that they clustered with Paenibacillus massiliensis 2301065T (95.9 and 94.3 % 16S rRNA gene sequence similarity, respectively, for strains 5402403T and 4401170T), Paenibacillus illinoisensis NRRL NRS-61356T (90.6 and 93.8 %), Paenibacillus xylanilyticus XIL14T (95.3 and 95.4 %), Paenibacillus barcinonensis BP-23T (94.3 and 94.0 %), Paenibacillus pabuli NCIMB 12781T (89.1 and 92.3 %) and Paenibacillus amylolyticus NRRL NRS-290T (94.2 and 93.8 %). The predominant fatty acids were 15 : 0 anteiso (49.0 and 55.3 %, respectively, for strains 5402403T and 4401170T), 16 : 0 iso (15.4 and 13.5 %), 16 : 0 (7.6 and 3.6 %), 15 : 0 (6.3 and 2.8 %), 17 : 0 anteiso (5.7 and 7.5 %), 14 : 0 iso (4.1 and 2.7 %) and 15 : 0 iso (4.1 and 3.4 %). 16S rRNA gene sequence similarity between strain 4401170T and strain 5402403T was 98.4 %, but the DNA–DNA reassociation rate between the two strains was 53.2 %. So, considering the recommendations of the ad hoc committee, they do not belong to the same species. On the basis of phenotypic data and genotypic inference, it is proposed that the strains should be assigned to the novel species Paenibacillus urinalis sp. nov. (type strain 5402403T =CIP 109357T =CCUG 53521T) and Paenibacillus provencensis sp. nov. (type strain 4401170T =CIP 109358T =CCUG 53519T).", }