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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 3

sp. nov., isolated from human cerebrospinal fluid, and sp. nov., isolated from human urine Free

Abstract

Gram-negative, spore-forming rods were isolated from a human urine sample (strain 5402403) and a human cerebrospinal fluid sample (strain 4401170). Based on genotypic characteristics, these strains belonged to the genus and were closely related. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that they clustered with 2301065 (95.9 and 94.3 % 16S rRNA gene sequence similarity, respectively, for strains 5402403 and 4401170), NRRL NRS-61356 (90.6 and 93.8 %), XIL14 (95.3 and 95.4 %), BP-23 (94.3 and 94.0 %), NCIMB 12781 (89.1 and 92.3 %) and NRRL NRS-290 (94.2 and 93.8 %). The predominant fatty acids were 15 : 0 anteiso (49.0 and 55.3 %, respectively, for strains 5402403 and 4401170), 16 : 0 iso (15.4 and 13.5 %), 16 : 0 (7.6 and 3.6 %), 15 : 0 (6.3 and 2.8 %), 17 : 0 anteiso (5.7 and 7.5 %), 14 : 0 iso (4.1 and 2.7 %) and 15 : 0 iso (4.1 and 3.4 %). 16S rRNA gene sequence similarity between strain 4401170 and strain 5402403 was 98.4 %, but the DNA–DNA reassociation rate between the two strains was 53.2 %. So, considering the recommendations of the ad hoc committee, they do not belong to the same species. On the basis of phenotypic data and genotypic inference, it is proposed that the strains should be assigned to the novel species sp. nov. (type strain 5402403 =CIP 109357 =CCUG 53521) and sp. nov. (type strain 4401170 =CIP 109358 =CCUG 53519).

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Keyword(s): CSF, cerebrospinal fluid
© SGM
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2008-03-01
2024-04-19
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Paenibacillus provencensis sp. nov., isolated from human cerebrospinal fluid, and Paenibacillus urinalis sp. nov., isolated from human urine
Int J Syst Evol Microbiol 58, 682 (2008); https://doi.org/10.1099/ijs.0.65228-0
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Supplements

vol. , part 3, pp. 682 - 687

Antibiotic susceptibility of sp. nov. 4401170 and sp. nov. 5402403 .

Extended phylogenetic tree of the genus inferred from 16S rRNA gene sequence comparison.

[PDF file of Supplementary Table S1 and Supplementary Fig. S1](26 KB)

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