1887

Abstract

Gram-negative, spore-forming rods were isolated from a human urine sample (strain 5402403) and a human cerebrospinal fluid sample (strain 4401170). Based on genotypic characteristics, these strains belonged to the genus and were closely related. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that they clustered with 2301065 (95.9 and 94.3 % 16S rRNA gene sequence similarity, respectively, for strains 5402403 and 4401170), NRRL NRS-61356 (90.6 and 93.8 %), XIL14 (95.3 and 95.4 %), BP-23 (94.3 and 94.0 %), NCIMB 12781 (89.1 and 92.3 %) and NRRL NRS-290 (94.2 and 93.8 %). The predominant fatty acids were 15 : 0 anteiso (49.0 and 55.3 %, respectively, for strains 5402403 and 4401170), 16 : 0 iso (15.4 and 13.5 %), 16 : 0 (7.6 and 3.6 %), 15 : 0 (6.3 and 2.8 %), 17 : 0 anteiso (5.7 and 7.5 %), 14 : 0 iso (4.1 and 2.7 %) and 15 : 0 iso (4.1 and 3.4 %). 16S rRNA gene sequence similarity between strain 4401170 and strain 5402403 was 98.4 %, but the DNA–DNA reassociation rate between the two strains was 53.2 %. So, considering the recommendations of the ad hoc committee, they do not belong to the same species. On the basis of phenotypic data and genotypic inference, it is proposed that the strains should be assigned to the novel species sp. nov. (type strain 5402403 =CIP 109357 =CCUG 53521) and sp. nov. (type strain 4401170 =CIP 109358 =CCUG 53519).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65228-0
2008-03-01
2020-09-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/3/682.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65228-0&mimeType=html&fmt=ahah

References

  1. Ash, C., Priest, F. G. & Collins, D. C. ( 1993; ). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposals for the creation of a new genus Paenibacillus. Antonie van Leeuwenhoek 64, 253–260.
    [Google Scholar]
  2. Bosshard, P. P., Zbinden, R. & Altwegg, M. ( 2002; ). Paenibacillus turicensis sp. nov., a novel bacterium harbouring heterogeneities between 16S rRNA genes. Int J Syst Evol Microbiol 52, 2241–2249.[CrossRef]
    [Google Scholar]
  3. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  4. Daane, L. L., Harjono, I., Barns, S. M., Launen, L. A., Palleroni, N. J. & Häggblom, M. M. ( 2002; ). PAH-degradation by Paenibacillus spp. and description of Paenibacillus naphthalenovorans sp. nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants. Int J Syst Evol Microbiol 52, 131–139.
    [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  6. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  7. Karlovsky, M. E. & Pontari, M. A. ( 2002; ). Theories of prostatitis etiology. Curr Urol Rep 3, 307–312.[CrossRef]
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  10. Nasu, Y., Nosaka, Y., Otsuka, Y., Tsuruga, T., Nakajima, M., Watanabe, Y. & Jin, M. ( 2003; ). A case of Paenibacillus polymyxa bacteraemia in a patient with cerebral infarction. Kansenshogaku Zasshi 77, 844–848 (in Japanese).[CrossRef]
    [Google Scholar]
  11. Noskin, G. A., Suriano, T., Collins, S., Sesler, S. & Peterson, L. R. ( 2001; ). Paenibacillus macerans pseudobacteremia resulting from contaminated blood culture bottles in a neonatal intensive care unit. Am J Infect Control 29, 126–129.[CrossRef]
    [Google Scholar]
  12. Rivas, R., Mateos, P. F., Martinez-Molina, E. & Velazquez, E. ( 2005; ). Paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium. Int J Syst Evol Microbiol 55, 405–408.[CrossRef]
    [Google Scholar]
  13. Roux, V. & Raoult, D. ( 2004; ). Paenibacillus massiliensis sp. nov., Paenibacillus sanguinis sp. nov. and Paenibacillus timonensis sp. nov., isolated from blood cultures. Int J Syst Evol Microbiol 54, 1049–1054.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Sánchez, M. M., Fritze, D., Blanco, A., Spröer, C., Tindall, B. J., Schumann, P., Kroppenstedt, R. M., Diaz, P. & Pastor, F. I. J. ( 2005; ). Paenibacillus barcinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the Ebro River delta. Int J Syst Evol Microbiol 55, 935–939.[CrossRef]
    [Google Scholar]
  16. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. ( 1997a; ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef]
    [Google Scholar]
  17. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. ( 1997b; ). Emended description of Paenibacillus amylolyticus and description of Paenibacillus illinoisensis sp. nov. and Paenibacillus chibensis sp. nov. Int J Syst Bacteriol 47, 299–306.[CrossRef]
    [Google Scholar]
  18. Teng, J. L. L., Woo, P. C. Y., Leung, K. W., Lau, S. K. P., Wong, M. K. M. & Yuen, K. Y. ( 2003; ). Pseudobacteraemia in a patient with neutropenic fever caused by a novel paenibacillus species: Paenibacillus hongkongensis sp. nov. Mol Pathol 56, 29–35.[CrossRef]
    [Google Scholar]
  19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  20. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  21. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65228-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65228-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 682 - 687

Antibiotic susceptibility of sp. nov. 4401170 and sp. nov. 5402403 .

Extended phylogenetic tree of the genus inferred from 16S rRNA gene sequence comparison.

[PDF file of Supplementary Table S1 and Supplementary Fig. S1](26 KB)



PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error