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Abstract

Strain Gsoil 259, a -glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the Republic of Korea and characterized in order to determine its taxonomic position. Cells were Gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16S rRNA gene sequence analysis revealed that strain Gsoil 259 belonged to the genus and was closely related to IFO 3750 (98.5 %) and IFO 12610 (97.9 %). However, it has low values for DNA–DNA relatedness with the above strains (20.7 and 17.5 %, respectively). Strain Gsoil 259 possessed chemotaxonomic markers that were consistent with classification in the genus , i.e. MK-11 and MK-12 were the major menaquinones and anteiso-C, anteiso-C and iso-C were the predominant cellular fatty acids. The DNA G+C content was 69.4 mol%. The cell-wall sugar was rhamnose and the diamino acid in the cell-wall peptidoglycan was ornithine. On the basis of data from this polyphasic study, strain Gsoil 259 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Gsoil 259 (=KCTC 19189 =DSM 18659).

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2008-02-01
2024-12-02
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Supplements

vol. , part 2, pp. 429 - 433

Extended neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, showing the phylogenetic relationships between strain Gsoil 259 and species of the genus .

Fatty acid compositions (%) for strain Gsoil 259 and its closest phylogenetic neighbours.

[PDF file for Supplementary Fig. S1 and Supplementary Table S1](54 KB)



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