1887

Abstract

We investigated the use of gene sequences as alternative phylogenetic and identification markers for vibrios. A fragment of 1322 bp (corresponding to approximately 88 % of the coding region) was analysed in 151 strains of vibrios. The relationships observed were in agreement with the phylogeny inferred from 16S rRNA gene sequence analysis. For instance, the , , and species groups appeared in the gene phylogenetic analyses, suggesting that these groups may be considered as separate genera within the current genus. Overall, gene sequences appeared to be more discriminatory for species differentiation than 16S rRNA gene sequences. 16S rRNA gene sequence similarities above 97 % corresponded to gene sequences similarities above 80 %. The intraspecies variation in the gene sequence was about 99 % sequence similarity. The results showed clearly that gene sequences are a suitable alternative for the identification and phylogenetic study of vibrios.

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2007-11-01
2019-10-21
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Supplements

A list of the strains used in this study. [ PDF] (74 KB)

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Regression curve between and 16S rRNA pairwise gene sequence similarity generated by Pearson's product-moment correlation coefficient using the software BioNumerics (Applied Maths). [ PDF] (127 KB)

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Phylogenetic tree based on the maximum-parsimony method, using gene sequences (1322 bp), of 151 representative strains of the family . [ PDF] (123 KB)

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