A novel moderately halophilic, Gram-positive bacterium, designated strain QW6, was isolated from textile industry wastewater in Qom, Iran. Strain QW6 was strictly aerobic, non-motile, non-sporulating and oxidase- and catalase-positive. It grew at salinities of 1–25 % (w/v) NaCl, showing optimal growth at 7.5–10.0 % (w/v). Growth occurred at 5.0–45.0 °C and over the pH range 6.5–10.0; growth was optimal at 35 °C and pH 7.5. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain QW6 is a member of the genus . The organism possessed Lys- and Gly-containing peptidoglycan. Strain QW6 had iso-C and anteiso-C as the predominant fatty acids and MK-6 as the major respiratory lipoquinone. The chemotaxonomic profile of this organism was consistent with its classification in the genus . The DNA G+C content of strain QW6 was 54.4 mol%. On the basis of phenotypic characteristics, 16S rRNA gene sequence analysis and DNA–DNA relatedness of less than 50 % to species of the genus , it is proposed that strain QW6 (=DSM 18903=CCM 7448) should be placed in the genus as the type strain of a novel species, sp. nov.


Article metrics loading...

Loading full text...

Full text loading...



  1. Aslam, Z., Lim, J. H., Im, W.-T., Yasir, M., Chung, Y. R. & Lee, S.-T.(2007).Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57, 633–638.[CrossRef] [Google Scholar]
  2. Baron, E. J. & Finegold, S. M.(1990).Bailey and Scott's Diagnostic Microbiology, 8th edn. St Louis: Mosby.
  3. Bozzola, J. J. & Russell, L. D.(1999).Electron Microscopy: Principles and Techniques for Biologists, 2nd edn. Sudbury, MA: Jones and Bartlett Publishers.
  4. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M.(1977). A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef] [Google Scholar]
  5. Chen, Y.-G., Cui, X.-L., Pukall, R., Li, H.-M., Yang, Y.-L., Xu, L.-H., Wen, M.-L., Peng, Q. & Jiang, C.-L.(2007).Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57, 2327–2332.[CrossRef] [Google Scholar]
  6. De Ley, J., Cattoir, H. & Reynaerts, R.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  7. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. França, L., Rainey, F. A., Nobre, M. F. & da Costa, M. S.(2006).Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate. Extremophiles 10, 531–536.[CrossRef] [Google Scholar]
  10. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  11. Harrigan, W. F. & McCance, M. E.(1976).Laboratory Methods in Food and Dairy Microbiology. London: Academic Press.
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  13. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  14. Kumar, S. K., Tamura, K. & Nei, M.(2004).mega3: an integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  15. Mata, J. A., Martinez-Conovasa, J., Quesada, E. & Bejar, V.(2002). A detailed phenotypic characterization of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef] [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  17. Monciardini, P., Cavaletti, L., Schumann, P., Rohde, M. & Donadio, S.(2003).Coexibacter woesei gen. nov., sp. nov., a novel representative of a deep evolutionary line of descent within the class Actinobacteria. Int J Syst Evol Microbiol 53, 569–576.[CrossRef] [Google Scholar]
  18. Murray, R. G. E., Doetsch, R. N. & Robinow, C. F.(1994). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  19. Pakdeeto, A., Tanasupawat, S., Thawai, C., Moonmangmee, S., Kudo, T. & Itoh, T.(2007).Salinicoccus siamensis sp. nov., isolated from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 57, 2004–2008.[CrossRef] [Google Scholar]
  20. Quesada, E., Ventosa, A., Ruiz-Berraquero, F. & Ramos-Cormenzana, A.(1984).Deleya halophila, a new species of moderately halophilic bacteria. Int J Syst Bacteriol 34, 287–292.[CrossRef] [Google Scholar]
  21. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  22. Schleifer, K. H.(1985). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156. [Google Scholar]
  23. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  25. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  26. Stead, D. E., Sellwood, J. E., Wilson, J. & Viney, I.(1992). Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J Appl Bacteriol 72, 315–321.[CrossRef] [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  28. Ventosa, A., Quesada, E., Rodríguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A.(1982). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968. [Google Scholar]
  29. Ventosa, A., Marquez, M. C., Ruiz-Berraquero, F. & Kocur, M.(1990).Salinicoccus roseus gen. nov., sp. nov., a new moderately halophilic Gram-positive coccus. Syst Appl Microbiol 13, 29–33.[CrossRef] [Google Scholar]
  30. Ventosa, A., Marquez, M. C., Weiss, N. & Tindall, B. J.(1992). Transfer of Marinococcus hispanicus to the genus Salinicoccus as Salinicoccus hispanicus comb. nov. Syst Appl Microbiol 15, 530–534.[CrossRef] [Google Scholar]
  31. Washington, J. A., II & Sutter, V. L.(1980). Dilution susceptibility test: agar and macro-broth dilution procedures. In Manual of Clinical Microbiology, 3rd edn, pp. 453–458. Edited by E. H. Lennette, A. Balows, W. J. Hausler, Jr & J. P. Truant. Washington, DC: American Society for Microbiology.
  32. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  33. Zhang, W., Xue, Y., Ma, Y., Zhou, P., Ventosa, A. & Grant, W. D.(2002).Salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from Baer soda lake in inner Mongolia autonomous region, China. Int J Syst Evol Microbiol 52, 789–793.[CrossRef] [Google Scholar]
  34. Zhang, Y.-Q., Yu, L.-Y., Liu, H.-Y., Zhang, Y.-Q., Xu, L.-H. & Li, W.-J.(2007).Salinicoccus luteus sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol 57, 1901–1905.[CrossRef] [Google Scholar]

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error