1887

Abstract

Two bacterial organisms, 50640 and 823, were isolated from fresh water in Marseilles, France, and were further identified as members of the genus on the basis of their phenotypic characteristics and 16S rRNA gene sequencing. Their unique phenotypic profile differed from that of closely related species of and by exhibiting positive indole and inositol tests, and from that of by lacking the ability to ferment -rhamnose. A polyphasic approach, including almost complete 16S rRNA gene sequencing (1461 bp) and partial sequencing of (683 bp), (662 bp), (624 bp) and (1049 bp) showed that isolates 50640 and 823 exhibited 98.5, 93.5, 90.4, 92.4 and 96.6 % similarity with , 98.7, 93.0, 90.1, 89.1 and 96.2 % with , and 98.4, 93.2, 89.8, 88.9 and 95.2 % with , respectively. Both isolates exhibited an identical 16S rRNA gene sequence and differed by one to five point mutations in housekeeping gene sequences. Phylogenetic reconstructions based on the combination of these four housekeeping genes indicated that the two isolates formed a unique branch supported by a bootstrap value of 93 %. Their unique phenotypic traits, 16S rRNA gene sequence, together with housekeeping gene sequences exhibiting <97 % similarity with closely related species, and phylogenetic analyses suggested that the two isolates represent a so far undescribed species. The name sp. nov. is proposed for this new taxon (type strain 50640=CIP 109351=CCUG 53443; isolate 823=CIP 109352=CCUG 53444).

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2008-04-01
2020-01-29
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vol. , part 4, pp. 779-784

Phylogenetic treesbased on , , and gene sequences of isolates 50640 and 823 and 11 species prepared by using the neighbour-joining method and Kimura’s two-parameter distance correction model. The support of each branch, as determined from 100 bootstrap samples, is indicated by the value (%) at each node. The scale bars represent the percentage difference in nucleotide sequence.



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