1887

Abstract

Fifteen strains of red-pigmented, strictly aerobic, coccoid, extremely halophilic archaea were isolated from fish sauce (nam-pla) produced in Thailand. They grew optimally at 37 °C, pH 6–8 and in the presence of 20–30 % (w/v) NaCl. The DNA G+C contents of the isolates were 60.0–61.8 mol%. They had MK-8(H) as a major menaquinone component and CC and CC derivatives of phosphatidylglycerol, phosphatidylglycerol methylphosphate and a sulfated glycolipid, S-DGA-1, as major polar lipid components. 16S rRNA gene sequence comparisons revealed that a representative strain, HDB5-2, was affiliated with JCM 12289, JCM 13587 and JCM 8876 (levels of similarity of 98.2–98.7 %). Based on data from DNA–DNA hybridization experiments, the 15 strains represented a single species, showing hybridization values of >78.9 % to representative strain HDB5-2, but were unrelated to either JCM 12289 or JCM 8876, with levels of relatedness of <50 %. Moreover, a comparison of phenotypic properties discriminated these new isolates from recognized species of the genus . The 15 strains are thus considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HDB5-2 (=BCC 20213 =JCM 13552 =PCU 278).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65218-0
2007-10-01
2020-09-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/10/2199.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65218-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Bohácek J., Kocur M., Martinec T. 1968; Deoxyribonucleic acid base composition of some marine and halophilic micrococci. J Appl Bacteriol 31:215–219 [CrossRef]
    [Google Scholar]
  3. Chaiyanan S., Chaiyanan S., Maugel T., Huq A., Robb F. T., Colwell R. R. 1999; Polyphasic taxonomy of a novel Halobacillus , Halobacillu s thailandensis sp. nov. isolated from fish sauce. Syst Appl Microbiol 22:360–365 [CrossRef]
    [Google Scholar]
  4. Denner E. B. M., McGenity T. J., Busse H.-J., Grant W. D., Wanner G., Stan-Lotter H. 1994; Halococcus salifodinae sp. nov., an archaeal isolate from an Austrian salt mine. Int J Syst Bacteriol 44:774–780 [CrossRef]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Goh F., Leuko S., Allen M. A., Bowman J. P., Kamekura M., Neilan B. A., Burns B. P. 2006; Halococcus hamelinensis sp. nov., a novel halophilic archaeon isolated from stromatolites in Shark Bay, Australia. Int J Syst Evol Microbiol 56:1323–1329 [CrossRef]
    [Google Scholar]
  8. Grant W. D. 2001; Genus Halococcus Schoop 1935a, 871AL. In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol 1 pp 311–314 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
  9. Grant W. D., Larsen H. 1989; Group III. Extremely halophilic archaeobacteria. Order Halobacteriales ord. nov. In Bergey's Manual of Systematic Bacteriology vol. 3 pp 2216–2219 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Kocur M., Hodgkiss W. 1973; Taxonomic status of the genus Halococcus Schoop. Int J Syst Bacteriol 23:151–156 [CrossRef]
    [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  12. Kumar S., Tamura K., Jakobson I.-B., Nei M. 2001; mega2: molecular evolution analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  14. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  15. Montero C. G., Ventosa A., Rodríguez-Valera F., Kates M., Moldoveanu N., Ruiz-Berraquero F. 1989; Halococcus saccharolyticus sp. nov., a new species of extremely halophilic non-alkaliphilic cocci. Syst Appl Microbiol 12:167–171 [CrossRef]
    [Google Scholar]
  16. Namwong S., Tanasupawat S., Smitinont T., Visessanguan W., Kudo T., Itoh T. 2005; Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. isolated from fish sauce in Thailand. Int J Syst Evol Microbiol 55:315–320 [CrossRef]
    [Google Scholar]
  17. Oren A., Ventosa A., Grant W. D. 1997; Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  18. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [CrossRef]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  20. Schoop G. 1935; Halococcus litoralis , ein obligat halophiler Farbstoffbildner. Dtsch Tierarztl Wochenschr 43:817–820 (in German
    [Google Scholar]
  21. Stan-Lotter H., Pfaffenhuemer M., Legat A., Busse H.-J., Radax C., Gruber C. 2002; Halococcus dombrowskii sp. nov., an archaeal isolate from a Permian alpine salt deposit. Int J Syst Evol Microbiol 52:1807–1814 [CrossRef]
    [Google Scholar]
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  23. Tanasupawat S., Komagata K. 2001; Lactic acid bacteria in fermented foods in Southeast Asia. In Microbial Diversity in Asia Technology and Prospects pp 43–59 Edited by Nga B. H., Tan H. M., Suzuki K. Singapore: World Scientific Publishing;
    [Google Scholar]
  24. Tanasupawat S., Shida O., Okada S., Komagata K. 2000; Lactobacillus acidipiscis sp. nov., and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50:1479–1485 [CrossRef]
    [Google Scholar]
  25. Tanasupawat S., Pakdeeto A., Namwong S., Thawai C., Kudo T., Itoh T. 2006; Lentibacillus halophilus sp. nov., from fish sauce in Thailand. Int J Syst Evol Microbiol 56:1859–1863 [CrossRef]
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  27. Thongsanit J., Tanasupawat S., Keeratipibul S., Jatikavanich S. 2002; Characterization and identification of Tetragenococcus halophilus and Tetragenococcus muriaticus strains from fish sauce (nam-pla). Jpn J Lactic Acid Bacteria 13:46–52 [CrossRef]
    [Google Scholar]
  28. Thongthai C., McGenity T. J., Suntinanalert P., Grant W. D. 1992; Isolation and characterization of an extremely halophilic archaeobacterium from traditionally fermented Thai fish sauce (nam pla). Lett Appl Microbiol 14:111–114 [CrossRef]
    [Google Scholar]
  29. Wang Q.-F., Li W., Yang H., Liu Y.-L., Cao H.-H., Dornmayr-Phaffenhuemer M., Stan-Lotter H., Guo G.-Q. 2007; Halococcus qingdaonensis sp. nov., a halophilic archaeon isolated from a crude sea-salt sample. Int J Syst Evol Microbiol 57:600–604 [CrossRef]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65218-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65218-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error