1887

Abstract

Fifteen strains of red-pigmented, strictly aerobic, coccoid, extremely halophilic archaea were isolated from fish sauce (nam-pla) produced in Thailand. They grew optimally at 37 °C, pH 6–8 and in the presence of 20–30 % (w/v) NaCl. The DNA G+C contents of the isolates were 60.0–61.8 mol%. They had MK-8(H) as a major menaquinone component and CC and CC derivatives of phosphatidylglycerol, phosphatidylglycerol methylphosphate and a sulfated glycolipid, S-DGA-1, as major polar lipid components. 16S rRNA gene sequence comparisons revealed that a representative strain, HDB5-2, was affiliated with JCM 12289, JCM 13587 and JCM 8876 (levels of similarity of 98.2–98.7 %). Based on data from DNA–DNA hybridization experiments, the 15 strains represented a single species, showing hybridization values of >78.9 % to representative strain HDB5-2, but were unrelated to either JCM 12289 or JCM 8876, with levels of relatedness of <50 %. Moreover, a comparison of phenotypic properties discriminated these new isolates from recognized species of the genus . The 15 strains are thus considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HDB5-2 (=BCC 20213 =JCM 13552 =PCU 278).

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2007-10-01
2019-12-06
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