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Volume 58, Issue 1

sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond Free

Abstract

A low-G+C, Gram-positive isolate, designated strain CVS-8, was isolated from a sea salt evaporation pond on the Island of Sal in the Cape Verde Archipelago. This organism was found to be a catalase- and oxidase-positive, non-motile, spore-forming, aerobic, curved rod-shaped organism with an optimum growth temperature of about 35–37 °C and an optimum pH between 7.5 and 8.0. Optimal growth occurred in media containing 4–6 % (w/v) NaCl and no growth occurred in medium without NaCl. The cell-wall peptidoglycan was of the A1 type with -diaminopimelic acid, the major respiratory quinone was MK-7, the major fatty acids were iso-15 : 0, 16 : 0, anteiso-15 : 0 and iso-16 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminoglycophospholipid. The G+C content of the DNA was 37.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CVS-8 represented a novel species of the genus , the highest levels of sequence similarity (mean pairwise similarity values of ∼97.5 %) being found with respect to the type strains of and . On the basis of the phylogenetic, physiological and biochemical data, strain CVS-8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CVS-8 (=LMG 22838=CIP 108578).

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2008-01-01
2024-04-18
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Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond
Int J Syst Evol Microbiol 58, 226 (2008); https://doi.org/10.1099/ijs.0.65217-0
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vol. , part 1, pp. 226 - 230

Maximum-parsimony phylogenetic dendrogram based on a comparison of the 16S rRNA gene sequences of strain CVS-8 and the closest phylogenetic relatives.

Effect of salt on growth of strain CVS-8 .

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