1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 1

sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond Free

Abstract

A low-G+C, Gram-positive isolate, designated strain CVS-8, was isolated from a sea salt evaporation pond on the Island of Sal in the Cape Verde Archipelago. This organism was found to be a catalase- and oxidase-positive, non-motile, spore-forming, aerobic, curved rod-shaped organism with an optimum growth temperature of about 35–37 °C and an optimum pH between 7.5 and 8.0. Optimal growth occurred in media containing 4–6 % (w/v) NaCl and no growth occurred in medium without NaCl. The cell-wall peptidoglycan was of the A1 type with -diaminopimelic acid, the major respiratory quinone was MK-7, the major fatty acids were iso-15 : 0, 16 : 0, anteiso-15 : 0 and iso-16 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminoglycophospholipid. The G+C content of the DNA was 37.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CVS-8 represented a novel species of the genus , the highest levels of sequence similarity (mean pairwise similarity values of ∼97.5 %) being found with respect to the type strains of and . On the basis of the phylogenetic, physiological and biochemical data, strain CVS-8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CVS-8 (=LMG 22838=CIP 108578).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65217-0
2008-01-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/1/226.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65217-0&mimeType=html&fmt=ahah

References

  1. Agnew, M. D., Koval, S. F. & Jarrell, K. F.(1995). Isolation and characterization of novel alkaliphiles from bauxite-processing waste and description of Bacillus vedderi sp. nov., a new obligate alkaliphile. Syst Appl Microbiol 18, 221–230.[CrossRef] [Google Scholar]
  2. Albert, R. A., Archambault, J., Rosselló-Mora, R., Tindall, B. J. & Matheny, M.(2005).Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55, 2125–2130.[CrossRef] [Google Scholar]
  3. Albuquerque, L., Simões, C., Nobre, M. F., Pino, N. M., Battista, J. R., Silva, M. T., Rainey, F. A. & da Costa, M. S.(2005).Truepera radiovictrix gen. nov., sp. nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov. FEMS Microbiol Lett 247, 161–169.[CrossRef] [Google Scholar]
  4. Arahal, D. R., Marquez, M. C., Volcani, B. E., Schleifer, K.-H. & Ventosa, A.(1999).Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J Syst Bacteriol 49, 521–530.[CrossRef] [Google Scholar]
  5. Degryse, E., Glansdorff, N. & Pierard, A.(1978). A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch Microbiol 117, 189–196.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Fujita, T., Shida, O., Takagi, H., Kunugita, K., Pankrushina, A. N. & Matsuhashi, M.(1996). Description of Bacillus carboniphilus sp. nov. Int J Syst Bacteriol 46, 116–118.[CrossRef] [Google Scholar]
  8. Logan, N. A.(2005). International Committee on Systematics of Prokaryotes; Subcommittee on the taxonomy of the genus Bacillus and related organisms: Minutes of the meeting, 30 July 2002, Paris, France. Int J Syst Evol Microbiol 55, 977–979.[CrossRef] [Google Scholar]
  9. Logan, N. A., Lebbe, L., Verhelst, A., Goris, J., Forsyth, G., Rodriguez-Diaz, M., Heyndrickx, M. & De Vos, P.(2004).Bacillus shackletonii sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. Int J Syst Evol Microbiol 54, 373–376.[CrossRef] [Google Scholar]
  10. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  12. Moreira, C., Rainey, F. A., Nobre, M. F., da Silva, M. T. & da Costa, M. S.(2000).Tepidimonas ignava gen. nov., sp. nov., a new chemolithoheterotrophic and slightly thermophilic member of the β-Proteobacteria. Int J Syst Evol Microbiol 50, 735–742.[CrossRef] [Google Scholar]
  13. Nielsen, P., Rainey, F. A., Outtrup, H., Priest, F. G. & Fritze, D.(1994). Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus. FEMS Microbiol Lett 117, 61–66.[CrossRef] [Google Scholar]
  14. Prado, A., da Costa, M. S. & Madeira, V. M. C.(1988). Effect of growth temperature on the lipid composition of two strains of Thermus sp. J Gen Microbiol 134, 1653–1660. [Google Scholar]
  15. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  16. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  17. Schleifer, K. H.(1985). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156. [Google Scholar]
  18. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  19. Tiago, I., Mendes, V., Pires, C., Morais, P. V. & Veríssimo, A.(2006).Chimaereicella alkaliphila gen. nov., sp. nov., a Gram-negative alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 29, 100–108.[CrossRef] [Google Scholar]
  20. Tindall, B. J.(1989). Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum. FEMS Microbiol Lett 60, 251–254.[CrossRef] [Google Scholar]
  21. Ventosa, A., Garcia, M. T., Kamekura, M., Onishi, H. & Ruiz-Berraquero, F.(1989).Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Syst Appl Microbiol 12, 162–165.[CrossRef] [Google Scholar]
  22. Yoon, J. H., Kim, I. G., Kang, K. H., Oh, T. K. & Park, Y. H.(2004).Bacillus hwajinpoensis sp. nov. and an unnamed Bacillus genomospecies, novel members of Bacillus rRNA group 6 isolated from sea water of the East Sea and the Yellow Sea in Korea. Int J Syst Evol Microbiol 54, 803–808.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.65217-0
Loading
Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond
Int J Syst Evol Microbiol 58, 226 (2008); https://doi.org/10.1099/ijs.0.65217-0
/content/journal/ijsem/10.1099/ijs.0.65217-0
/content/journal/ijsem/10.1099/ijs.0.65217-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 226 - 230

Maximum-parsimony phylogenetic dendrogram based on a comparison of the 16S rRNA gene sequences of strain CVS-8 and the closest phylogenetic relatives.

Effect of salt on growth of strain CVS-8 .

[ Combined PDF] (31 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error