1887

Abstract

A Gram-negative, non-spore-forming, rod-shaped, motile bacterium, designated strain 43, was isolated from a Japanese salty food and then subjected to a polyphasic taxonomic study. Strain 43 is moderately halophilic, growing at NaCl concentrations in the range 5–25 % (w/v), with optimum growth between 7.5 and 12.5 % (w/v) NaCl. Growth occurs at temperatures from 15 to 42 °C (optimally at 28–37 °C) and at pH 5.5–9.0 (optimally at pH 7.0–8.0). A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain 43 belongs to the genus . The closest relatives were ATCC 43984 (99.3 % 16S rRNA gene sequence similarity), ATCC 19372 (99.1 %), LV4 (98.3 %), LTS-4N (97.9 %) and ATCC 17056 (97.9 %). The DNA G+C content was 62.9 mol%, which is within the range described for the genus . DNA–DNA hybridization studies between strain 43 and CECT 5385 and DSM 7218 showed 38 and 49 % relatedness, respectively; lower DNA–DNA hybridization percentages were obtained with respect to other related species. The major fatty acids of strain 43 were C, C cyclo 8 and C 3-OH. Overall, the phenotypic, genotypic and phylogenetic results demonstrated that strain 43 represents a novel species within the genus . The name sp. nov. is proposed, with strain 43 (=CECT 7219 =CCM 7416) as the type strain.

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2007-10-01
2021-03-06
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