1887

Abstract

A Gram-negative, non-spore-forming, rod-shaped, motile bacterium, designated strain 43, was isolated from a Japanese salty food and then subjected to a polyphasic taxonomic study. Strain 43 is moderately halophilic, growing at NaCl concentrations in the range 5–25 % (w/v), with optimum growth between 7.5 and 12.5 % (w/v) NaCl. Growth occurs at temperatures from 15 to 42 °C (optimally at 28–37 °C) and at pH 5.5–9.0 (optimally at pH 7.0–8.0). A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain 43 belongs to the genus . The closest relatives were ATCC 43984 (99.3 % 16S rRNA gene sequence similarity), ATCC 19372 (99.1 %), LV4 (98.3 %), LTS-4N (97.9 %) and ATCC 17056 (97.9 %). The DNA G+C content was 62.9 mol%, which is within the range described for the genus . DNA–DNA hybridization studies between strain 43 and CECT 5385 and DSM 7218 showed 38 and 49 % relatedness, respectively; lower DNA–DNA hybridization percentages were obtained with respect to other related species. The major fatty acids of strain 43 were C, C cyclo 8 and C 3-OH. Overall, the phenotypic, genotypic and phylogenetic results demonstrated that strain 43 represents a novel species within the genus . The name sp. nov. is proposed, with strain 43 (=CECT 7219 =CCM 7416) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65212-0
2007-10-01
2020-01-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/10/2262.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65212-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R. & Ventosa, A. ( 2006; ). The family Halomonadaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 6, pp. 811–835. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  2. Arahal, D. R., Garcia, M. T., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2001a; ). Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51, 1443–1448.
    [Google Scholar]
  3. Arahal, D. R., Garcia, M. T., Vargas, C., Canovas, D., Nieto, J. J. & Ventosa, A. ( 2001b; ). Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174. Int J Syst Evol Microbiol 51, 1457–1462.
    [Google Scholar]
  4. Arahal, D. R., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2002; ). Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. Int J Syst Evol Microbiol 52, 241–249.
    [Google Scholar]
  5. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M. ( 1966; ). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45, 493–496.
    [Google Scholar]
  6. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. De Ley, J. & Tijtgat, R. ( 1970; ). Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36, 461–474.[CrossRef]
    [Google Scholar]
  8. Elazari-Volcani, B. ( 1940; ). Studies on the microflora of the Dead Sea. PhD thesis, Hebrew University of Jerusalem.
  9. Garrity, G. M., Bell, J. A. & Lilburn, T. ( 2005; ). Family IV. Halomonadaceae Franzmann, Wehmeyer and Stackebrandt 1989, 205VP emend. Dobson and Franzmann 1996, 558. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, p. 300. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  10. Hof, T. ( 1935; ). An investigation of the microorganisms commonly present in salted beans. Rec Trav Bot Neerl 32, 151–173.
    [Google Scholar]
  11. Johnson, J. L. ( 1994; ). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–681. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  12. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  13. Ludwig, W., Strunk, O., Klugbauer, S., Klugbauer, N., Weizenernegger, M., Neumaier, J., Bachleitner, M. & Schleifer, K.-H. ( 1998; ). Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554–568.[CrossRef]
    [Google Scholar]
  14. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  15. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  16. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  17. Mellado, E., Moore, E. R. B., Nieto, J. J. & Ventosa, A. ( 1995; ). Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45, 712–716.[CrossRef]
    [Google Scholar]
  18. Miller, L. T. ( 1982; ). Single derivation method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxyl acids. J Clin Microbiol 16, 584–586.
    [Google Scholar]
  19. Mormile, M. R., Romine, M. F., Garcia, M. T., Ventosa, A., Bailey, T. J. & Peyton, B. M. ( 1999; ). Halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium. Syst Appl Microbiol 22, 551–558.[CrossRef]
    [Google Scholar]
  20. Onishi, H., Fuchi, H., Konomi, K., Hidaka, O. & Kamekura, M. ( 1980; ). Isolation and distribution of a variety of halophilic bacteria and their classification by salt-response. Agric Biol Chem 44, 1253–1258.[CrossRef]
    [Google Scholar]
  21. Owen, R. J. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists, 2nd edn, pp. 277–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  22. Peçonek, J., Gruber, C., Gallego, V., Ventosa, A., Busse, H.-J., Kämpfer, P., Radax, C. & Stan-Lotter, H. ( 2006; ). Reclassification of Pseudomonas beijerinckii Hof 1935 as Chromohalobacter beijerinckii comb. nov., and emended description of the species. Int J Syst Evol Microbiol 56, 1953–1957.[CrossRef]
    [Google Scholar]
  23. Prado, B., Lizama, C., Aguiler, M., Ramos-Cormenzana, A., Fuentes, S., Campos, V. & Monteoliva-Sanchez, M. ( 2006; ). Chromohalobacter nigrandesensis sp. nov., a moderately halophilic, Gram-negative bacterium isolated from Lake Tebenquiche on the Atacama Saltern, Chile. Int J Syst Evol Microbiol 56, 647–651.[CrossRef]
    [Google Scholar]
  24. Quillaguamán, J., Delgado, O., Mattiasson, B. & Hatti-Kaul, R. ( 2004; ). Chromohalobacter sarecensis sp. nov., a psychrotolerant moderate halophile isolated from the saline Andean region of Bolivia. Int J Syst Evol Microbiol 54, 1921–1926.[CrossRef]
    [Google Scholar]
  25. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E., Fredericksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  27. Ventosa, A. ( 2005; ). Genus III. Chromohalobacter Ventosa, Gutierrez, García and Ruiz-Berraquero 1989, 384VP emend. Arahal, García, Ludwig, Schleifer and Ventosa 2001a, 1446. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 316–319. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  28. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  29. Ventosa, A., Gutierrez, M. C., Garcia, M. T. & Ruiz-Berraquero, F. ( 1989; ). Classification of “Chromobacterium marismortui” in a new genus, Chromohalobacter gen. nov., as Chromohalobacter marismortui comb. nov., nom. rev. Int J Syst Bacteriol 39, 382–386.[CrossRef]
    [Google Scholar]
  30. Ventosa, A., Gutierrez, M. C., Kamekura, M. & Dyall-Smith, M. L. ( 1999; ). Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 49, 131–136.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65212-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65212-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error