A novel actinomycete, designated strain YIM 46034, was isolated from an evergreen broadleaved forest at Menghai, in southern Yunnan Province, China. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that the strain belonged to the family . Strain YIM 46034 showed more than 3 % 16S rRNA gene sequence divergence from recognized species of genera in the family . Characteristic features of strain YIM 46034 were the production of two types of spores, namely motile spores, which were formed in sporangia produced on substrate mycelia, and single globose spores, which were observed on short sporophores of the substrate mycelia. The cell wall contained -diaminopimelic acid, glycine, arabinose and xylose, which are characteristic components of cell-wall chemotype II of actinomycetes. Phosphatidylethanolamine was the major phospholipid (phospholipid type II). Based on morphological, chemotaxonomic, phenotypic and genetic characteristics, strain YIM 46034 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is YIM 46034 (=CCTCC AA 205013 =DSM 44991).


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vol. , part 6, pp. 1324 - 1331

Scanning electron micrographs of strain YIM 46034 grown on GYM agar. (a) Finger-like sporangia (arrows) growing directly on substrate mycelium; (b) rod-like motile sporangiospores (flagella not visible); (c) globose, non-motile spores arising from short sporophores of the substrate mycelium.

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