1887

Abstract

Phenotypic and phylogenetic studies were performed on 15 isolates of an unidentified Gram-positive, anaerobic, non-sporulating coccobacillus-shaped bacterium isolated from human faeces. The novel organisms were catalase-negative, indole-negative and produced acetate and succinate as end products of metabolism. Comparative 16S rRNA gene sequencing demonstrated that the 15 isolates were highly related to each other and formed a hitherto unknown subline within the clostridial rRNA cluster XIVa. The novel isolates formed a robust phylogenetic group with a number of organisms which included , , and a number of other misclassified ruminococci. On the basis of these studies, a novel genus, gen. nov., is proposed. It is suggested that , , , , , and are transferred to this genus as gen. nov., comb. nov., comb. nov., comb. nov., comb. nov., comb. nov. and comb. nov. One of the new isolates, the hitherto unknown coccus-shaped bacterial strain WAL 14507 (=ATCC BAA-1564=DSM 19850) is proposed as representing the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65208-0
2008-08-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/8/1896.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65208-0&mimeType=html&fmt=ahah

References

  1. Barcenilla, A., Pryde, S. E., Martin, J. C., Duncan, S. H., Stewart, C. S., Henderson, C. & Flint, H. J.(2000). Phylogenetic relationships of butyrate-producing bacteria from the human gut. Appl Environ Microbiol 66, 1654–1661.[CrossRef] [Google Scholar]
  2. Bernalier, A., Willems, A., Leclerc, M., Rochet, V. & Collins, M. D.(1996).Ruminococcus hydrogenotrophicus sp. nov., a new H2/CO2-utilizing acetogenic bacterium isolated from human feces. Arch Microbiol 166, 176–183.[CrossRef] [Google Scholar]
  3. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F.(1978). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef] [Google Scholar]
  4. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J. & Farrow, J. A. E.(1994). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef] [Google Scholar]
  5. Ezaki, T., Li, N., Hashimoto, Y., Miura, H. & Yamamoto, H.(1994). 16S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov. Int J Syst Bacteriol 44, 130–136.[CrossRef] [Google Scholar]
  6. Ezaki, T., Li, N. & Kawamura, Y.(2006). The anaerobic gram-positive cocci. In The Prokaryotes, vol. 6, pp. 795–808. Edited by M. Dworkin, S. Fallow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer-Verlag.
  7. Finegold, S. M., Molitoris, D., Song, Y. L., Liu, C. X., Vaisanen, M. L., Bolte, E., McTeague, M., Sandler, R., Wexler, H. & other authors(2002). Gastrointestinal microflora studies in late-onset autism. Clin Infect Dis 35 (Suppl. 1), S6–S16.[CrossRef] [Google Scholar]
  8. Holdeman, L. V., Cato, E. P. & Moore, W. E. C.(1977).Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  9. Holdeman, L. V. & Moore, W. E. C.(1974). New genus, Coprococcus, twelve new species, and emended descriptions of bacteria from human feces. Int J Syst Bacteriol 24, 260–277.[CrossRef] [Google Scholar]
  10. Jousimies-Somer, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. & Finegold, S. M.(2002).Wadsworth-KTL Anaerobic Bacteriology Manual, 6th edn. Belmont, CA: Star Publishing Co.
  11. Kaneuchi, C., Benno, Y. & Mitsuoka, T.(1976).Clostridium coccoides, a new species from the feces of mice. Int J Syst Bacteriol 26, 482–486.[CrossRef] [Google Scholar]
  12. Liou, J. S.-C., Balkwill, D. L., Drake, G. R. & Tanner, R. S.(2005).Clostridium carboxidivorans sp. nov., a solvent-producing clostridium isolated from an agricultural settling lagoon, and reclassification of the acetogen Clostridium scatologenes strain SL-1 as Clostridium drakei sp. nov. Int J Syst Evol Microbiol 55, 2085–2091.[CrossRef] [Google Scholar]
  13. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  14. Pearson, W. R. & Lipman, D. J.(1988). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef] [Google Scholar]
  15. Rainey, F. A. & Janssen, P. H.(1995). Phylogenetic analysis by 16S ribosomal DNA sequence comparison reveals two unrelated groups of species within the genus Ruminococcus. FEMS Microbiol Lett 129, 69–73. [Google Scholar]
  16. Rieu-Lesme, F., Morvan, B., Collins, M. D., Fonty, G. & Willems, A.(1996). A new H2/CO2-using acetogenic bacterium from the rumen: description of Ruminococcus schinkii sp. nov. FEMS Microbiol Lett 140, 281–286. [Google Scholar]
  17. Simmering, R., Taras, D., Schwiertz, A., Le Blay, G., Gruhl, B., Lawson, P. A., Collins, M. D. & Blaut, M.(2002).Ruminococcus luti sp. nov., isolated from a human faecal sample. Syst Appl Microbiol 25, 189–193.[CrossRef] [Google Scholar]
  18. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155. [Google Scholar]
  19. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  20. Suau, A., Bonnet, R., Sutren, M., Godon, J.-J., Gibson, G. R., Collins, M. D. & Doré, J.(1999). Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65, 4799–4807. [Google Scholar]
  21. Wexler, H. M., Reeves, D., Summanen, P. H., Molitoris, E., McTeague, M., Duncan, J., Wilson, K. H. & Finegold, S. M.(1996).Sutterella wadsworthensis gen. nov., sp. nov., bile-resistant microaerophilic Campylobacter gracilis-like clinical isolates. Int J Syst Bacteriol 46, 252–258.[CrossRef] [Google Scholar]
  22. Willems, A. & Collins, M. D.(1995). Phylogenetic analysis of Ruminococcus flavefaciens, the type species of the genus Ruminococcus does not support the reclassification of Streptococcus hansenii and Peptostreptococcus productus as ruminococci. Int J Syst Bacteriol 45, 572–575.[CrossRef] [Google Scholar]
  23. Zoetendal, E. G., Ben-Amor, K., Harmsen, H. J. M., Schut, F., Akkermans, A. D. L. & de Vos, W. M.(2002). Quantification of uncultured Ruminococcus obeum-like bacteria in human fecal samples by fluorescent in situ hybridization and flow cytometry using 16S rRNA-targeted probes. Appl Environ Microbiol 68, 4225–4232.[CrossRef] [Google Scholar]
  24. Zoetendal, E. G., Akkermans, A. D. L. & de Vos, W. M.(1998). Temperature gradient gel electrophoresis analysis of 16s rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol 64, 3854–3859. [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.65208-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65208-0
Loading

Data & Media loading...

Supplements

vol. , part 8, pp. 1896 - 1902

Unrooted phylogenetic tree showing the inter-relationships of sp. nov. with some of its nearest relatives within the rRNA cluster XIVa. [ PDF] 71 KB



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error