A Gram-type negative or variable, aerobic, rod-shaped, nitrogen-fixing bacterium, designated strain CC4, was isolated on nutrient agar from a soil sample collected at a regional agricultural research field station located in Kaohsiung County, Taiwan. 16S rRNA gene sequence analysis demonstrated that this isolate is unique, showing <92.5 % similarity with respect to species of the genera (maximum of 92.2 % similarity), (92.0 %), (91.9 %), (91.9 %) and (91.9 %). The organism utilized acetic acid, formic acid, -hydroxybutyric acid, -lactic acid, succinic acid, bromosuccinic acid, -alaninamide, -alanine, -alanyl glycine, -glutamic acid and -proline as substrates, but not methanol or methylamine. Chemotaxonomic data revealed that strain CC4 contains ubiquinone Q-10 as the major respiratory quinone. The characteristic diamino acid of the peptidoglycan was -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, an unknown lipid and an unknown aminolipid. The fatty acid profile differed from those of members of the genera , , and . The predominant fatty acids were C 7, C cyclo and C. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and physiological data, strain CC4 represents a novel genus and species, for which we propose the name gen. nov., sp. nov. The type strain of is CC4 (=DSM 19599 =CIP 109513).


Article metrics loading...

Loading full text...

Full text loading...



  1. Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef] [Google Scholar]
  2. Hahn, T.-W., Willby, M. J. & Krause, D. C.(1998). HMW1 is required for cytadhesin P1 trafficking to the attachment organelle in Mycoplasma pneumoniae. J Bacteriol 180, 1270–1276. [Google Scholar]
  3. Im, W. T., Yokota, A., Kim, M. K. & Lee, S. T.(2004).Kaistia adipata gen. nov., sp. nov., a novel alpha-proteobacterium. J Gen Appl Microbiol 50, 249–254.[CrossRef] [Google Scholar]
  4. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  5. Kämpfer, P., Steiof, M. & Dott, W.(1991). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef] [Google Scholar]
  6. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  7. Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef] [Google Scholar]
  8. Peña, A., Valens, M., Santos, F., Buczolits, S., Antón, J., Kämpfer, P., Busse, H.-J., Amann, R. & Rosselló-Mora, R.(2005). Intraspecific comparative analysis of the species Salinibacter ruber. Extremophiles 9, 151–161.[CrossRef] [Google Scholar]
  9. Powers, E. M.(1995). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61, 3756–3758. [Google Scholar]
  10. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S.(1955). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844–4846.[CrossRef] [Google Scholar]
  11. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  12. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  13. Tindall, B. J.(1990a). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef] [Google Scholar]
  14. Tindall, B. J.(1990b). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef] [Google Scholar]
  15. Wiegel, J. K. W.(2005). Genus XX. Xanthobacter Wiegel, Wilke, Baumgarten, Opitz and Schlegel 1978, 573AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 555–566. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  16. Wiegel, J. K. W. & Schlegel, H. G.(1984). Genus Xanthobacter Wiegel, Wilke, Baumgarten, Opitz and Schlegel 1978, 573AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 325–333. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  17. Yoon, J.-H., Kang, S.-J., Park, S. & Oh, T. K.(2007).Devosia insulae sp. nov., isolated from soil of Dokdo in Korea, and emended description of the genus Devosia. Int J Syst Evol Microbiol 57, 1310–1314.[CrossRef] [Google Scholar]
  18. Young, C.-C., Kämpfer, P., Shen, F.-T., Lai, W.-A. & Arun, A. B.(2005).Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55, 423–426.[CrossRef] [Google Scholar]

Data & Media loading...


vol. , part 7, pp. 1571 - 1575

Scanning electron micrograph of cells of strain CC4 . Bar, 1 µm.


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error