1887

Abstract

A Gram-positive, short rod-shaped, non-spore-forming bacterium, designated 5516T-20, was isolated from an air sample. The results of 16S rRNA gene sequence analyses showed that strain 5516T-20 belonged to the family , having the highest sequence similarities (97.5 and 96.1 %, respectively) with respect to the type strains of and . The value for DNA–DNA hybridization between 5516T-20 and DS-8 was 41 %. Strain 5516T-20 contained menaquinone MK-8(H) as the major isoprenoid quinone, possessed phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol as the polar lipids and contained glucose and ribose as whole-cell sugars. The major fatty acids were C 8, iso-C, iso-C, C and iso-C. The G+C content of the DNA from strain 5516T-20 was 70.5 mol%. On the basis of the data from the polyphasic taxonomic study, strain 5516T-20 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 5516T-20 (=KACC 20658 =DSM 18548).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65202-0
2008-10-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/10/2389.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65202-0&mimeType=html&fmt=ahah

References

  1. DSMZ ( 2001; ). Catalogue of Strains, 7th edn. Braunschweig: Deutsche Sammlung von Mikroorganismen und Zellkulturen.
  2. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  3. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D. H., Lim, C. K. & Go, S. J. ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  4. Lee, S. D. ( 2006; ). Phycicoccus jejuensis gen. nov., sp. nov., an actinomycete isolated from seaweed. Int J Syst Evol Microbiol 56, 2369–2373.[CrossRef]
    [Google Scholar]
  5. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  6. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef]
    [Google Scholar]
  7. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  8. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  9. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  10. Seldin, L. & Dubnau, D. ( 1985; ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef]
    [Google Scholar]
  11. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  12. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  13. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  14. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  15. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  16. Yoon, J.-H., Lee, S.-Y., Kang, S.-J. & Oh, T.-K. ( 2008; ). Phycicoccus dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 58, 597–600.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65202-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65202-0
Loading

Data & Media loading...

Supplements

vol. , part 10, pp. 2389 - 2392

Cellular fatty acid profiles of strain 5516T-20 , DS-8 and KSW2-15 . [PDF](34 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error