1887

Abstract

The type strain of and 38 strains identified as , four strains of , including the type and two subcultures of the type, two strains of and six unidentified strains that resembled were examined by PCR fingerprints using primers M13 and (GAC)5. The same strains, together with four strains of the recently introduced nom. inval., were sequenced for the D1/D2 domain of the 26S rRNA gene and parts of the ITS domain and also studied for their physiological properties. Of the strains identified previously as , CBS 2076 had the same fingerprint as the type of and strain CBS 4855 was distinct from all other strains. The six strains that resembled were separated into two groups distinct from any of the other clades. A total of six groups obtained by fingerprint and sequence data were evaluated by performing DNA reassociation reactions. Mating experiments among the 35 strains of showed that 15 strains represented one mating type and 16 strains represented the opposite mating type, while four strains were self-sporulating. Teleomorph states were not produced by , or any of the unidentified isolates. However, positive mating reactions were rarely observed in crosses among and some strains of Y. and . Consequently, sharing the same mating type system, and could be considered anamorphs of unnamed species. Results from PCR fingerprints, sequencing and mating studies support the grouping of the studied strains into , , , nom. inval. and three novel species in the clade: sp. nov. (type strain CBS 10146 =NRRL Y-48252; Mycobank number MB 510769), sp. nov. (type strain CBS 10151 =NRRL Y-48253; Mycobank number MB 510770) and sp. nov. (type strain CBS 4855 =NRRL Y-48254; Mycobank number MB 510771).

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2007-10-01
2019-10-23
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vol. , part 10, pp. 2426 - 2435

Inter- and intragroup genome variation between strains of and related species based on mean DNA–DNA reassociation values.

Intragenomic differences in ITS sequences of some strains.

[PDF file of Supplementary Figs S1 and S2](99 KB)



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