%0 Journal Article %A Park, Dong-Jin %A Dastager, Syed G. %A Lee, Jae-Chan %A Yeo, Soo-Hwan %A Yoon, Jung-Hoon %A Kim, Chang-Jin %T Shimazuella kribbensis gen. nov., sp. nov., a mesophilic representative of the family Thermoactinomycetaceae %D 2007 %J International Journal of Systematic and Evolutionary Microbiology, %V 57 %N 11 %P 2660-2664 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.65194-0 %I Microbiology Society, %X A mesophilic strain, designated A 9500T, was isolated from a soil sample collected from Sobaek Mountain, South Korea, and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew well on different ISP media and no diffusible pigments were produced. The optimum temperature for growth was 32 °C. The aerial mycelium was well developed, but not fragmented. The strain was Gram-positive, non-motile and formed endospores on vegetative and aerial hyphae with a spiny surface. Cell walls of strain A 9500T contained meso-diaminopimelic acid as the diagnostic amino acid, but no characteristic sugars or other amino acids were found (chemotype-III). The major menaquinone was MK-9(H4) and the minor menaquinone was MK-10(H4) and they were detected at a ratio of 7 : 3. Phosphatidylethanolamine was the diagnostic phospholipid. The G+C content of the genomic DNA was 39.4 mol%. The major fatty acids were anteiso-C15 : 0 (43.34 %), iso-C16 : 0 (14.23 %) and C16 : 0 (7.90 %), a composition that differed from members of related genera of the family Thermoactinomycetaceae. The novel strain formed a distinct clade in a phylogenetic tree based on 16S rRNA gene sequences. On the basis of a range of phenotypic and genotypic data and on 16S rRNA gene sequence similarities (88.35–90.38 %), it is suggested that strain A 9500T represents a novel species in a new genus with the name Shimazuella kribbensis gen. nov., sp. nov. The type strain of the type species is A 9500T (=KCTC 9933T=DSM 45090T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65194-0