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Abstract

A mesophilic strain, designated A 9500, was isolated from a soil sample collected from Sobaek Mountain, South Korea, and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew well on different ISP media and no diffusible pigments were produced. The optimum temperature for growth was 32 °C. The aerial mycelium was well developed, but not fragmented. The strain was Gram-positive, non-motile and formed endospores on vegetative and aerial hyphae with a spiny surface. Cell walls of strain A 9500 contained -diaminopimelic acid as the diagnostic amino acid, but no characteristic sugars or other amino acids were found (chemotype-III). The major menaquinone was MK-9(H) and the minor menaquinone was MK-10(H) and they were detected at a ratio of 7 : 3. Phosphatidylethanolamine was the diagnostic phospholipid. The G+C content of the genomic DNA was 39.4 mol%. The major fatty acids were anteiso-C (43.34 %), iso-C (14.23 %) and C (7.90 %), a composition that differed from members of related genera of the family . The novel strain formed a distinct clade in a phylogenetic tree based on 16S rRNA gene sequences. On the basis of a range of phenotypic and genotypic data and on 16S rRNA gene sequence similarities (88.35–90.38 %), it is suggested that strain A 9500represents a novel species in a new genus with the name gen. nov., sp. nov. The type strain of the type species is A 9500 (=KCTC 9933=DSM 45090).

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2007-11-01
2020-01-27
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