1887

Abstract

Strain BCRC 14193, isolated from soil, shared more than 99 % 16S rRNA gene sequence similarity with BCRC 11601 and BCRC 17467. This strain was previously identified as , based on DNA–DNA hybridization, but its DNA relatedness value with BCRC 17467 was 89 %. To investigate the relatedness of strain BCRC 14193, and , the partial sequence of the gene encoding the subunit B protein of DNA gyrase () was determined. BCRC 17467 shared high gene sequence similarity with BCRC 14193 (98.4 %) and all of the strains available (95.5–95.6 %). DNA–DNA hybridization experiments revealed high relatedness values between BCRC 17467 and BCRC 11601 (74 %) and the reference strains (74–89 %). Based on these data and the lack of phenotypic distinctive characteristics, we propose as a later heterotypic synonym of .

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2008-03-01
2019-10-22
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References

  1. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 2002; ). phylip (Phylogeny Inference Package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  3. Fukumoto, J. ( 1943a; ). Studies on the production of bacterial amylase. I. Isolation of bacteria secreting potent amylase and their distribution. Nippon Nogei Kaggakai 19, 487–503 (in Japanese).[CrossRef]
    [Google Scholar]
  4. Fukumoto, J. ( 1943b; ). Studies on the production of bacterial amylase. II. Bacterial and physiological nature. Nippon Nogei Kaggakai 19, 643–650 (in Japanese).
    [Google Scholar]
  5. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  6. Harwood, C. R. ( 1992; ). Bacillus subtilis and its relatives: molecular biological and industrial workhorses. Trends Biotechnol 10, 247–256.[CrossRef]
    [Google Scholar]
  7. Kostinek, M., Pukall, R., Rooney, A. P., Schillinger, U., Hertel, C., Holzapfel, W. H. & Franz, C. M. ( 2005; ). Lactobacillus arizonensis is a later heterotypic synonym of Lactobacillus plantarum. Int J Syst Evol Microbiol 55, 2485–2489.[CrossRef]
    [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  9. Logan, N. A. & Berkeley, R. C. ( 1984; ). Identification of Bacillus strains using the API system. J Gen Microbiol 130, 1871–1882.
    [Google Scholar]
  10. Nakamura, L. K. ( 1987; ). Deoxyribonucleic acid relatedness of lactose-positive Bacillus subtilis strains and Bacillus amyloliquefaciens. Int J Syst Bacteriol 37, 444–445.[CrossRef]
    [Google Scholar]
  11. Nakamura, L. K. ( 1989; ). Taxonomic relationship of black-pigmented Bacillus subtilis strains and a proposal for Bacillus atrophaeus sp. nov. Int J Syst Bacteriol 39, 295–300.[CrossRef]
    [Google Scholar]
  12. Nakamura, L. K., Roberts, M. S. & Cohan, F. M. ( 1999; ). Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov. Int J Syst Bacteriol 49, 1211–1215.[CrossRef]
    [Google Scholar]
  13. Naser, S. M., Hagen, K. E., Vancanneyt, M., Cleenwerck, I., Swings, J. & Tompkins, T. A. ( 2006a; ). Lactobacillus suntoryeus Cachat and Priest 2005 is a later synonym of Lactobacillus helveticus (Orla-Jensen 1919) Bergey et al. 1925 (Approved Lists 1980). Int J Syst Evol Microbiol 56, 355–360.[CrossRef]
    [Google Scholar]
  14. Naser, S. M., Vancanneyt, M., Hoste, B., Snauwaert, C., Vandemeulebroecke, K. & Swings, J. ( 2006b; ). Reclassification of Enterococcus flavescens Pompei et al. 1992 as a later synonym of Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984 and Enterococcus saccharominimus Vancanneyt et al. 2004 as a later synonym of Enterococcus italicus Fortina et al. 2004. Int J Syst Evol Microbiol 56, 413–416.[CrossRef]
    [Google Scholar]
  15. O'Donnell, A. G., Norris, J. R., Berkeley, R. C. W., Kaneko, Logan, N. A. & Nozaki, R. ( 1980; ). Characterization of Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, and Bacillus amyloliquefaciens by pyrolysis gas-liquid chromatography, deoxyribonucleic acid-deoxyribonucleic acid hybridization, biochemical tests, and API systems. Int J Syst Bacteriol 30, 448–459.[CrossRef]
    [Google Scholar]
  16. Priest, F. G. ( 1977; ). Extracellular enzyme synthesis in the genus Bacillus. Bacteriol Rev 41, 711–753.
    [Google Scholar]
  17. Priest, F. G., Goodfellow, M., Shute, L. A. & Berkeley, R. C. W. ( 1987; ). Bacillus amyloliquefaciens sp. nov., nom. rev. Int J Syst Bacteriol 37, 69–71.[CrossRef]
    [Google Scholar]
  18. Roberts, M. S., Nakamura, L. K. & Cohan, F. M. ( 1994; ). Bacillus mojavensis sp. nov., distinguishable from Bacillus subtilis by sexual isolation, divergence in DNA sequence, and differences in fatty acid composition. Int J Syst Bacteriol 44, 256–264.[CrossRef]
    [Google Scholar]
  19. Roberts, M. S., Nakamura, L. K. & Cohan, F. M. ( 1996; ). Bacillus vallismortis sp. nov., a close relative of Bacillus subtilis, isolated from soil in Death Valley, California. Int J Syst Bacteriol 46, 470–475.[CrossRef]
    [Google Scholar]
  20. Ruiz-García, C., Bejar, V., Martinez-Checa, F., Llamas, I. & Quesada, E. ( 2005; ). Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain. Int J Syst Evol Microbiol 55, 191–195.[CrossRef]
    [Google Scholar]
  21. Skerman, V. B. D., McGowan, V. & Sneath, P. H. A. (editors) 1980; ). Approved lists of bacterial names. Int J Syst Bacteriol 30, 225–420.[CrossRef]
    [Google Scholar]
  22. Tai, C. J., Kuo, H. P., Lee, F. L., Chen, H. K., Yokota, A. & Lo, C. C. ( 2006; ). Chryseobacterium taiwanense sp. nov., isolated from soil in Taiwan. Int J Syst Evol Microbiol 56, 1771–1776.[CrossRef]
    [Google Scholar]
  23. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  24. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  25. Wang, L. T., Lee, F. L., Tai, C. J., Yokota, A. & Kuo, H. P. ( 2007a; ). Reclassification of Bacillus axarquiensis Ruiz-García et al. 2005 and Bacillus malacitensis Ruiz-García et al. 2005 as later heterotypic synonyms of Bacillus mojavensis Roberts et al. 1994. Int J Syst Evol Microbiol 57, 1663–1667.[CrossRef]
    [Google Scholar]
  26. Wang, L. T., Lee, F. L., Tai, C. J. & Kasai, H. ( 2007b; ). Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA–DNA hybridization in the Bacillus subtilis group. Int J Syst Evol Microbiol 57, 1846–1850.[CrossRef]
    [Google Scholar]
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Maximum-parsimony, maximum-likelihood and neighbour-joining trees obtained with 16S rRNA gene sequence, gene sequence and GyrB protein sequence of selected Bacillus strains. [PDF](414 KB)

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Cellular fatty acid composition and differential phenotypic characteristics of and related species [PDF](68 KB)

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