sp. nov., isolated from a marine solar saltern in Korea Free

Abstract

A Gram-negative, motile, rod-shaped, -like bacterial strain, ISL-52, was isolated from a marine solar saltern of the Yellow Sea in Korea and was subjected to a polyphasic taxonomic investigation. Strain ISL-52 grew optimally at pH 7.0–8.0 and at 30–37 °C. It contained Q-8 as the predominant ubiquinone. The major fatty acids (>10 % of total fatty acids) were iso-C, iso-C and iso-C 9. The DNA G+C content was 53.9 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-52 fell within the genus , joining the type strain of at a bootstrap resampling value of 100 %. Strain ISL-52 exhibited 16S rRNA gene sequence similarity values of 94.9–96.7 % with respect to the type strains of eight recognized species. The differential phenotypic properties of ISL-52, together with its phylogenetic distinctiveness, demonstrated that this strain is distinguishable from the recognized species. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain ISL-52 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ISL-52 (=KCTC 12971=CCUG 54359).

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2007-11-01
2024-03-28
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References

  1. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium ,Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes . pp 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer-Verlag;
  2. Brettar I., Christen R., Höfle M. G. 2003; Idiomarina baltica sp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 53:407–413 [CrossRef]
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  4. Choi D. H., Cho B. C. 2005; Idiomarina seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea. Int J Syst Evol Microbiol 55:379–383 [CrossRef]
    [Google Scholar]
  5. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [CrossRef]
    [Google Scholar]
  6. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  7. Donachie S. P., Hou S., Gregory T. S., Malahoff A., Alam M. 2003; Idiomarina loihiensis sp. nov., a halophilic γ - Proteobacterium from the Lō'ihi submarine volcano. Hawai'i. Int J Syst Evol Microbiol 53:1873–1879 [CrossRef]
    [Google Scholar]
  8. Ivanova E. P., Romanenko L. A., Chun J., Matte M. H., Matte G. R., Mikhailov V. V., Svetashev V. I., Huq A., Maugel T., Colwell R. R. 2000; Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp.nov. and Idiomarina zobellii sp. nov.. Int J Syst Evol Microbiol 50:901–907 [CrossRef]
    [Google Scholar]
  9. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  10. Kwon S.-W., Kim B.-Y., Weon H.-Y., Baek Y.-K., Koo B.-S., Go S.-J. 2006; Idiomarina homiensis sp. nov., isolated from seashore sand in Korea. Int J Syst Evol Microbiol 56:2229–2233 [CrossRef]
    [Google Scholar]
  11. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  12. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  13. Martínez-Cánovas M. J., Béjar V., Martínez-Checa F., Páez R., Quesada E. 2004; Idiomarina fontislapidosi sp. nov. and Idiomarina ramblicola sp. nov., isolated from inland hypersaline habitats in Spain. Int J Syst Evol Microbiol 54:1793–1797 [CrossRef]
    [Google Scholar]
  14. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids , MIDI. Technical Note 101: Newark, DE: MIDI;
    [Google Scholar]
  15. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  16. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  17. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  18. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  19. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  20. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef]
    [Google Scholar]
  21. Yurkov V., Stackebrandt E., Holmes A., Fuerst J. A., Hugenholtz P., Golecki J., Gad'on N., Gorlenko V. M., Kompantseva E. I., Drews G. 1994; Phylogenetic positions of novel aerobic, bacteriochlorophyll a -containing bacteria and description of Roseococcus thiosulfatophilus gen.nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov.. Int J Syst Bacteriol 44:427–434 [CrossRef]
    [Google Scholar]
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