1887

Abstract

A Gram-negative, short rod-shaped, nitrogen-fixing bacterium (CC-21) was isolated from a soil sample collected from the regional agricultural research station in Kaohsiung County (Taiwan). Using 16S rRNA gene sequence analysis, it could be clearly demonstrated that this isolate was novel: it showed <97 % similarity to species of the genera , , , and . The organism used several organic acids, but only a few sugars as substrates. The fatty acid profile differed from those reported for members of the genera , , , and . On the basis of 16S rRNA gene sequence analysis in combination with physiological data, strain CC-21 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain is CC-21 (=CCUG 54492=CIP 109510).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65175-0
2008-02-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/2/491.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65175-0&mimeType=html&fmt=ahah

References

  1. Burris, R. H. ( 1972; ). Nitrogen fixation assay – methods and techniques. Methods Enzymol 24, 415–431.
    [Google Scholar]
  2. Gardan, L., Stead, D. E., Dauga, C. & Gillis, M. ( 2003; ). Acidovorax valerianellae sp. nov., a novel pathogen of lamb's lettuce [Valerianella locusta (L.) Laterr.]. Int J Syst Evol Microbiol 53, 795–800.[CrossRef]
    [Google Scholar]
  3. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  4. Grabovich, M., Gavrish, E., Kuever, J., Lysenko, A. M., Podkopaeva, D. & Dubinina, G. ( 2006; ). Proposal of Giesbergeria voronezhensis gen. nov., sp. nov. and G. kuznetsovii sp. nov. and reclassification of [Aquaspirillum] anulus, [A.] sinuosum and [A.] giesbergeri as Giesbergeria anulus comb. nov., G. sinuosa comb. nov. and G. giesbergeri comb. nov. and [Aquaspirillum] metamorphum and [A.] psychrophilum as Simplicispira metamorpha gen. nov., comb. nov. and S. psychrophila comb. nov. Int J Syst Evol Microbiol 56, 569–576.[CrossRef]
    [Google Scholar]
  5. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  6. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  7. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J. ( 2003; ). Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef]
    [Google Scholar]
  8. Khan, S. T. & Hiraishi, A. ( 2002; ). Diaphorobacter nitroreducens gen. nov., sp. nov. a poly(3-hydoxybutyrate)-degrading denitrifying bacterium isolated from activated sludge. J Gen Appl Microbiol 48, 299–308.[CrossRef]
    [Google Scholar]
  9. Krieg, N. R. ( 1984; ). Genus Aquaspirillum Hylemon, Wells, Krieg and Jannasch 1973, 361. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 72–90. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  11. Malik, K. A. & Schlegel, H. G. ( 1981; ). Chemolithoautotrophic growth of bacteria able to grow under N2-fixing conditions. FEMS Microbiol Lett 11, 63–67.[CrossRef]
    [Google Scholar]
  12. Mechichi, T., Stackebrandt, E. & Fuchs, G. ( 2003; ). Alicycliphilus denitrificans gen. nov., sp. nov., a cyclohexanol-degrading, nitrate-reducing β-proteobacterium. Int J Syst Evol Microbiol 53, 147–152.[CrossRef]
    [Google Scholar]
  13. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  14. Poly, F., Monrozier, L. J. & Bally, R. ( 2001; ). Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol 152, 95–103.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Sakane, T. & Yokota, A. ( 1994; ). Chemotaxonomic investigation of heterotrophic, aerobic and microaerobic spirilla, the genera Aquaspirillum, Magnetospirillum and Oceanospirillum. Syst Appl Microbiol 17, 128–134.[CrossRef]
    [Google Scholar]
  17. Schulze, R., Spring, S., Amann, R., Huber, I., Ludwig, W., Schleifer, K.-H. & Kämpfer, P. ( 1999; ). Genotypic diversity of Acidovorax strains isolated from activated sludge and description of Acidovorax defluvii sp. nov. Syst Appl Microbiol 22, 205–214.[CrossRef]
    [Google Scholar]
  18. Spring, S., Jäckel, U., Wagner, M. & Kämpfer, P. ( 2004; ). Ottowia thiooxydans gen. nov., sp. nov., a novel facultatively anaerobic, N2O-producing bacterium isolated from activated sludge, and transfer of Aquaspirillum gracile to Hylemonella gracilis gen. nov., comb. nov. Int J Syst Evol Microbiol 54, 99–106.[CrossRef]
    [Google Scholar]
  19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  20. Wen, A., Fegan, M., Hayward, C., Chakraborty, S. & Sly, L. I. ( 1999; ). Phylogenetic relationships among members of the Comamonadaceae, and description of Delftia acidovorans (den Dooren de Jong 1926 and Tamaoka et al. 1987) gen. nov., comb. nov. Int J Syst Bacteriol 49, 567–576.[CrossRef]
    [Google Scholar]
  21. Willems, A., Falsen, E., Pot, B., Jantzen, E., Hoste, B., Vandamme, P., Gillis, M., Kersters, K. & De Ley, J. ( 1990; ). Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb. nov., and Acidovorax temperans sp. nov. Int J Syst Bacteriol 40, 384–398.[CrossRef]
    [Google Scholar]
  22. Young, C.-C., Kämpfer, P., Shen, F.-T., Lai, W.-A. & Arun, A. B. ( 2005; ). Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55, 423–426.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65175-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65175-0
Loading

Data & Media loading...

Supplements

vol. , part 2, pp. 491 - 495

gene sequence-based neighbour-joining tree showing the relationship between strain CC-21 ( gen. nov., sp. nov.) and other dinitrogen-fixing bacteria. [PDF](83 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error