1887

Abstract

Five isolates that were related phylogenetically to members of the genus were isolated from freshwater of the hard-water creek Westerhöfer Bach, North Germany. The five strains (WB 2.1-25, WB 2.3-71, WB 3.3-3, WB 3.3-22 and WB 2.3-45) were Gram-negative and chemoheterotrophic, with rod-shaped cells. Most of their metabolic properties matched those given in the description of the genus . Consistent with the genus description, their fatty acids included mainly iso-C and summed feature 3 (C 7, iso-C 2-OH or both); C 5, C, iso-C 3-OH, C 3-OH and iso-C 3-OH were present in smaller amounts. The major isoprenoid quinone was menaquinone 7. With one exception, binary similarity values of the almost complete 16S rRNA gene sequences determined among the isolates as well as between the isolates and type strains of species were lower than 98.5 %. The only exception was the close relationship between DSM 16990 and strain WB 2.3-45 (99.2 % similarity). DNA–DNA reassociation values determined for this pair of strains was 29.8 %, indicating that strain WB 2.3-45 represents a unique genospecies. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains WB 2.1-25 and WB 2.3-71 form a group that is moderately related to and strain WB 2.3-45 (98.5 % similarity). Strains WB 3.3-3 and WB 3.3-22 (98.5 % similarity) branched separately from these four organisms. The five phylogenetically isolated strains differed from each other as well as from the type strain of the type species ( DSM 2366) and some related representatives of the genus in several metabolic reactions and cultural parameters. On the basis of phenotypic and phylogenetic distinctiveness, five novel species are proposed: sp. nov., with WB 2.1-25 (=DSM 19034=CIP 109481) as the type strain; sp. nov., with WB 3.3-22 (=DSM 19036=CIP 109479) as the type strain; sp. nov., with WB 2.3-71 (=DSM 19035=CIP 109480) as the type strain; sp. nov., with WB 3.3-3 (=DSM 19033=CIP 109468) as the type strain; and sp. nov., with WB 2.3-45 (=DSM 19110=CIP 109507) as the type strain.

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2007-10-01
2019-10-19
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