1887

Abstract

Three strains of budding, ellipsoid-shaped and rosette-forming bacteria were isolated from acidic -dominated boreal wetlands of northern Russia and were designated strains MPL7, MOB77 and SB2. The presence of crateriform pits and numerous fibrillar appendages on the cell surface and an unusual spur-like projection on one pole of the cell indicated a planctomycete morphotype. These isolates are moderately acidophilic, mesophilic organisms capable of growth at pH values between 4.2 and 7.5 (with an optimum at pH 5.0–6.2) and at temperatures between 4 and 32 °C (optimum 15–26 °C). The major fatty acids are C and C 7; the major quinone is MK-6. The G+C content of the DNA is 54.4–56.5 mol%. Strains MPL7, MOB77 and SB2 possess nearly identical 16S rRNA gene sequences and belong to the planctomycete lineage defined by the genus , being most closely related to DSM 3776 (86.9–87.1 % sequence similarity). However, strain MPL7 showed only 28 % DNA–DNA hybridization with DSM 3776. Compared with currently described members of the genus , the isolates from northern wetlands do not form long and distinctive stalks, have greater tolerance of acidic conditions and low temperatures, are more sensitive to NaCl, lack pigmentation and degrade a wider range of biopolymers. The data therefore suggest that strains MPL7, MOB77 and SB2 represent a novel genus and species, for which the name gen. nov., sp. nov., is proposed. Strain MPL7 (=ATCC BAA-1393 =VKM B-2452) is the type strain of .

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2007-11-01
2019-12-07
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vol. , part 11, pp. 2680 - 2687

Electron micrographs of negatively stained cells displaying fimbria-like fibrillar structures and thicker fibrillar appendages on the cell surface of strain MPL7 .

Influence of medium pH and temperature on the growth of strain MPL7 .

[PDF file of Supplementary Figs S1 and S2](69 KB)



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