Six representatives of a luminous bacterium commonly found in association with deep, cold-dwelling marine fishes were isolated from the light organs and skin of different fish species. These bacteria were Gram-negative, catalase-positive, and weakly oxidase-positive or oxidase-negative. Morphologically, cells of these strains were coccoid or coccoid-rods, occurring singly or in pairs, and motile by means of polar flagellation. After growth on seawater-based agar medium at 22 °C for 18 h, colonies were small, round and white, with an intense cerulean blue luminescence. Analysis of 16S rRNA gene sequence similarity placed these bacteria in the genus . Phylogenetic analysis based on seven housekeeping gene sequences (16S rRNA gene, , , , , and ), seven gene sequences of the operon (, , , , , and ) and four gene sequences of the operon (, , and ), resolved the six strains as members of the genus and as a clade distinct from other species of . These strains were most closely related to and . DNA–DNA hybridization values between the designated type strain, .1.1, and . LMG 4233, . LMG 19543 and LMG 22857 were 51, 43 and 19 %, respectively. In AFLP analysis, the six strains clustered together, forming a group distinct from other analysed species. The fatty acid C cyclo was present in these bacteria, but not in . , . or . A combination of biochemical tests (arginine dihydrolase and lysine decarboxylase) differentiates these strains from . and . . The DNA G+C content of .1.1 is 40.2 %, and the genome size is approximately 4.2 Mbp, in the form of two circular chromosomes. These strains represent a novel species, for which the name sp. nov. is proposed. The type strain, .1.1 (=ATCC BAA-1194=LMG 23890), is a luminous symbiont isolated from the light organ of the deep-water fish .


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vol. , part 9, pp. 2073 - 2078

– Methods

Phylogenetic tree based on analysis of three genes ( , and )

. Primer sequences, PCR conditions and references for amplification of genetic sequences used in this study

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