A Gram-negative, microaerophilic slender rod, measuring approximately 10 μm long and approximately 1 μm wide, isolated from the gastric mucosa of a cat and designated strain M50, was subjected to a polyphasic taxonomic study. Despite its apparent lack of helical coils, the organism showed a corkscrew-like motion by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril coiled around the body. Strain M50 grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominated in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 37 °C, but not at 25 or 42 °C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus and showed about 98 to 99 % sequence similarity to , and ‘ Helicobacter heilmannii’, five highly related species previously detected in the feline or canine gastric mucosa. Protein profiling of strain M50 using SDS-PAGE revealed a pattern different from those of other species of mammalian gastric origin. Additionally, the urease and HSP60 gene sequences of strain M50 were different from those of , and ‘ H. heilmannii’. It is thus proposed that strain M50 (=LMG 23839=CCUG 53816) represents a novel species within this genus, for which the name sp. nov. is proposed.


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vol. , part 2, pp. 357 - 364

Distance matrices of strains based on 16S rRNA, and gene sequences. [ Combined PDF] 220 KB.

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