1887

Abstract

A Gram-negative, microaerophilic slender rod, measuring approximately 10 μm long and approximately 1 μm wide, isolated from the gastric mucosa of a cat and designated strain M50, was subjected to a polyphasic taxonomic study. Despite its apparent lack of helical coils, the organism showed a corkscrew-like motion by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril coiled around the body. Strain M50 grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominated in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 37 °C, but not at 25 or 42 °C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus and showed about 98 to 99 % sequence similarity to , and ‘ Helicobacter heilmannii’, five highly related species previously detected in the feline or canine gastric mucosa. Protein profiling of strain M50 using SDS-PAGE revealed a pattern different from those of other species of mammalian gastric origin. Additionally, the urease and HSP60 gene sequences of strain M50 were different from those of , and ‘ H. heilmannii’. It is thus proposed that strain M50 (=LMG 23839=CCUG 53816) represents a novel species within this genus, for which the name sp. nov. is proposed.

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2008-02-01
2020-01-18
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References

  1. Andersen, L. P., Boye, K., Blom, J., Holck, S., Norgaard, A. & Elsborg, L. ( 1999; ). Characterization of a culturable “Gastrospirillum hominis” (Helicobacter heilmannii) strain isolated from human gastric mucosa. J Clin Microbiol 37, 1069–1076.
    [Google Scholar]
  2. Baele, M., Devriese, L. A. & Haesebrouck, F. ( 2001; ). Lactobacillus agilis is an important component of the pigeon crop flora. J Appl Microbiol 91, 488–491.[CrossRef]
    [Google Scholar]
  3. Baele, M., Van den Bulck, K., Decostere, A., Vandamme, P., Hanninen, M. L., Ducatelle, R. & Haesebrouck, F. ( 2004; ). Multiplex PCR assay for differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis. J Clin Microbiol 42, 1115–1122.[CrossRef]
    [Google Scholar]
  4. Bronsdon, M. A., Goodwin, C. S., Sly, L. I., Chilvers, T. & Schoenknecht, F. D. ( 1991; ). Helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (Macaca nemestrina). Int J Syst Bacteriol 41, 148–153.[CrossRef]
    [Google Scholar]
  5. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R. W., Kersters, K. & Vandamme, P. ( 1999; ). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef]
    [Google Scholar]
  6. Dewhirst, F. E., Fox, J. G., Mendes, E. N., Paster, B. J., Gates, C. E., Kirkbride, C. A. & Eaton, K. A. ( 2000a; ).Flexispira rappini’ strains represent at least 10 Helicobacter taxa. Int J Syst Evol Microbiol 50, 1781–1787.
    [Google Scholar]
  7. Dewhirst, F. E., Fox, J. G. & On, S. L. W. ( 2000b; ). Recommended minimal standards for describing new species of the genus Helicobacter. Int J Syst Evol Microbiol 50, 2231–2237.[CrossRef]
    [Google Scholar]
  8. Dewhirst, F. E., Shen, Z. L., Scimeca, M. S., Stokes, L. N., Boumenna, T., Chen, T. T., Paster, B. J. & Fox, J. G. ( 2005; ). Discordant 16S and 23S rRNA gene phylogenies for the genus Helicobacter: implications for phylogenetic inference and systematics. J Bacteriol 187, 6106–6118.[CrossRef]
    [Google Scholar]
  9. Eaton, K. A., Dewhirst, F. E., Radin, M. J., Fox, J. G., Paster, B. J., Krakowka, S. & Morgan, D. R. ( 1993; ). Helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. Int J Syst Bacteriol 43, 99–106.[CrossRef]
    [Google Scholar]
  10. Eaton, K. A., Dewhirst, F. E., Paster, B. J., Tzellas, N., Coleman, B. E., Paola, J. & Sherding, R. ( 1996; ). Prevalence and varieties of Helicobacter species in dogs from random sources and pet dogs: animal and public health implications. J Clin Microbiol 34, 3165–3170.
    [Google Scholar]
  11. Fox, J. G., Taylor, N. S., Edmonds, P. & Brenner, D. J. ( 1988; ). Campylobacter pylori subsp. mustelae subsp. nov., isolated from the gastric mucosa of ferrets (Mustela putorius furo), and an emended description of Campylobacter pylori. Int J Syst Bacteriol 38, 367–370.[CrossRef]
    [Google Scholar]
  12. Fox, J. G., Yan, L. L., Dewhirst, F. E., Paster, B. J., Shames, B., Murphy, J. C., Howard, A., Belcher, J. C. & Mendes, E. N. ( 1995; ). Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice. J Clin Microbiol 33, 445–454.
    [Google Scholar]
  13. Gruntar, I., Mehle, J., Krt, B., Ocepek, M., Gombac, M., Cerne, M. & Pogacnik, M. ( 2003; ). New approaches in isolation of Helicobacter spp. from gastric mucosa in dogs. Int J Med Microbiol 293 (Suppl.), 65
    [Google Scholar]
  14. Hänninen, M. L., Happonen, I., Saari, S. & Jalava, K. ( 1996; ). Culture and characteristics of Helicobacter bizzozeronii, a new canine gastric Helicobacter sp. Int J Syst Bacteriol 46, 160–166.[CrossRef]
    [Google Scholar]
  15. Hänninen, M. L., Utriainen, M., Happonen, I. & Dewhirst, F. E. ( 2003; ). Helicobacter sp. flexispira 16S rDNA taxa 1, 4 and 5 and Finnish porcine Helicobacter isolates are members of the species Helicobacter trogontum (taxon 6). Int J Syst Evol Microbiol 53, 425–433.[CrossRef]
    [Google Scholar]
  16. Hänninen, M. L., Kärenlampi, R. I., Koort, J. M. K., Mikkonen, T. & Björkroth, K. J. ( 2005; ). Extension of the species Helicobacter bilis to include the reference strains of Helicobacter sp. flexispira taxa 2, 3 and 8 and Finnish canine and feline flexispira strains. Int J Syst Evol Microbiol 55, 891–898.[CrossRef]
    [Google Scholar]
  17. Houf, K., On, S. L. W., Coenye, T., Mast, J., Van Hoof, J. & Vandamme, P. ( 2005; ). Arcobacter cibarius sp nov., isolated from broiler carcasses. Int J Syst Evol Microbiol 55, 713–717.[CrossRef]
    [Google Scholar]
  18. Jalava, K., Kaartinen, M., Utriainen, M., Happonen, I. & Hanninen, M. L. ( 1997; ). Helicobacter salomonis sp. nov., a canine gastric Helicobacter sp. related to Helicobacter felis and Helicobacter bizzozeronii. Int J Syst Bacteriol 47, 975–982.[CrossRef]
    [Google Scholar]
  19. Jalava, K., On, S. L. W., Vandamme, P. A. R., Happonen, I., Sukura, A. & Hanninen, M. L. ( 1998; ). Isolation and identification of Helicobacter spp. from canine and feline gastric mucosa. Appl Environ Microbiol 64, 3998–4006.
    [Google Scholar]
  20. Jalava, K., On, S. L. W., Harrington, C. S., Andersen, L. P., Hanninen, M. L. & Vandamme, P. ( 2001; ). A cultured strain of “Helicobacter heilmannii”, a human gastric pathogen, identified as H. bizzozeronii: evidence for zoonotic potential of Helicobacter. Emerg Infect Dis 7, 1036–1038.[CrossRef]
    [Google Scholar]
  21. Lee, A., Hazell, S. L., O'Rourke, J. & Kouprach, S. ( 1988; ). Isolation of a spiral-shaped bacterium from the cat stomach. Infect Immun 56, 2843–2850.
    [Google Scholar]
  22. Lee, A., Phillips, M. W., O'Rourke, J. L., Paster, B. J., Dewhirst, F. E., Fraser, G. J., Fox, J. G., Sly, L. I., Romaniuk, P. J. & other authors ( 1992; ). Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int J Syst Bacteriol 42, 27–36.[CrossRef]
    [Google Scholar]
  23. Mast, J., Nanbru, C., van den Berg, T. & Meulemans, G. ( 2005; ). Ultrastructural changes of the tracheal epithelium after vaccination of day-old chickens with the La Sota strain of Newcastle disease virus. Vet Pathol 42, 559–565.[CrossRef]
    [Google Scholar]
  24. Mendes, E. N., Queiroz, D. M. M., Dewhirst, F. E., Paster, B. J., Moura, S. B. & Fox, J. G. ( 1996; ). Helicobacter trogontum sp. nov., isolated from the rat intestine. Int J Syst Bacteriol 46, 916–921.[CrossRef]
    [Google Scholar]
  25. Mikkonen, T. P., Karenlampi, R. I. & Hänninen, M. L. ( 2004; ). Phylogenetic analysis of gastric and enterohepatic Helicobacter species based on partial HSP60 gene sequences. Int J Syst Evol Microbiol 54, 753–758.[CrossRef]
    [Google Scholar]
  26. Mills, C. K. & Gherna, R. L. ( 1987; ). Hydrolysis of indoxyl acetate by Campylobacter species. J Clin Microbiol 25, 1560–1561.
    [Google Scholar]
  27. O'Rourke, J. L., Solnick, J. V., Neilan, B. A., Seidel, K., Hayter, R., Hansen, L. M. & Lee, A. ( 2004; ). Description of ‘Candidatus Helicobacter heilmannii’ based on DNA sequence analysis of 16S rRNA and urease genes. Int J Syst Evol Microbiol 54, 2203–2211.[CrossRef]
    [Google Scholar]
  28. Patterson, M. M., Schrenzel, M. D., Feng, Y., Xu, S., Dewhirst, F. E., Paster, B. J., Thibodeau, S. A., Versalovic, J. & Fox, J. G. ( 2000; ). Helicobacter aurati sp. nov., a urease-positive Helicobacter species cultured from gastrointestinal tissues of Syrian hamsters. J Clin Microbiol 38, 3722–3728.
    [Google Scholar]
  29. Pot, B., Devriese, L. A., Hommez, J., Miry, C., Vandemeulebroecke, K., Kersters, K. & Haesebrouck, F. ( 1994; ). Characterization and identification of Vagococcus fluvialis strains isolated from domestic animals. J Appl Bacteriol 77, 362–369.[CrossRef]
    [Google Scholar]
  30. Solnick, J. V., O'Rourke, J., Lee, A., Paster, B. J., Dewhirst, F. E. & Tompkins, L. S. ( 1993; ). An uncultured gastric spiral organism is a newly identified Helicobacter in humans. J Infect Dis 168, 379–385.[CrossRef]
    [Google Scholar]
  31. Van den Bulck, K., Decostere, A., Baele, M., Driessen, A., Debongnie, J. C., Burette, A., Stolte, M., Ducatelle, R. & Haesebrouck, F. ( 2005; ). Identification of non-Helicobacter pylori spiral organisms in gastric samples from humans, dogs and cats. J Clin Microbiol 43, 2256–2260.[CrossRef]
    [Google Scholar]
  32. Van den Bulck, K., Decostere, A., Baele, M., Vandamme, P., Mast, J., Ducatelle, R. & Haesebrouck, F. ( 2006; ). Helicobacter cynogastricus sp. nov., isolated from the canine gastric mucosa. Int J Syst Evol Microbiol 56, 1559–1564.[CrossRef]
    [Google Scholar]
  33. Vandamme, P., Pot, B., Gillis, M., De Vos, P., Kersters, K. & Swings, J. ( 1996; ). Polyphasic taxonomy, a consensus approach to bacterial classification. Microbiol Rev 60, 407–438.
    [Google Scholar]
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vol. , part 2, pp. 357 - 364

Distance matrices of strains based on 16S rRNA, and gene sequences. [ Combined PDF] 220 KB.



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