1887

Abstract

Two Gram-positive, coccoid, non-spore-forming bacteria (strains 3-M5-R-4 and 3-M5-R-7), cells of which formed diploid, tetrad and cluster arrangements, were isolated from air of the Regenerative Enclosed Life Support Module Simulator system. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the family and were related to members of the genus , with similarities to the seven known species of the genus of 96.71–97.76 %. The fatty acid profile supported affiliation of these novel isolates to the genus , although larger amounts of octadecanoic acid (C) and -9-octadecenoic acid (C) were observed in the isolates, thus enabling them to be differentiated from other species. In addition, DNA–DNA hybridization studies indicated that these strains belonged to a novel species that could be readily distinguished from its nearest neighbour, DSM 13192, which had less than 20 % DNA–DNA relatedness. Physiological and biochemical tests showed few phenotypic differences, but genotypic analysis enabled these gelatin-liquefying strains to be differentiated from the seven species. The strains described in this study therefore represent a novel species, for which the name sp. nov. is proposed; the type strain is 3-M5-R-4 (=ATCC BAA-1496 =CIP 109413).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65137-0
2007-12-01
2020-11-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/12/2749.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65137-0&mimeType=html&fmt=ahah

References

  1. Blackall L. L., Seviour E. M., Bradford D., Rossetti S., Tandoi V., Seviour R. J. 2000; Candidatus Nostocoida limicola’, a filamentous bacterium from activated sludge. Int J Syst Evol Microbiol 50:703–709 [CrossRef]
    [Google Scholar]
  2. Carter L., Tabb D., Tatara J. D., Mason R. K. 2005; Performance qualification test of the ISS Water Processor Assembly (WPA) expendables. In Proceedings of the 35th International Conference on Environmental Systems 11–14 July 2005 Rome, Italy: SAE Technical Paper 2005-01-2837
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Fernandez-Garayzabal J. F., Dominguez L., Pascual C., Jones D., Collins M. D. 1995; Phenotypic and phylogenetic characterization of some unknown coryneform bacteria isolated from bovine blood and milk: description of Sanguibacter gen. nov. Lett Appl Microbiol 20:69–75 [CrossRef]
    [Google Scholar]
  5. Hanada S., Liu W. T., Shintani T., Kamagata Y., Nakamura K. 2002; Tetrasphaera elongata sp. nov., a polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Evol Microbiol 52:883–887 [CrossRef]
    [Google Scholar]
  6. Ishikawa T., Yokota A. 2006; Reclassification of Arthrobacter duodecadis Lochhead 1958 as Tetrasphaera duodecadis comb. nov. and emended description of the genus Tetrasphaera . Int J Syst Evol Microbiol 56:1369–1373 [CrossRef]
    [Google Scholar]
  7. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  8. Mastronarde D. N. 1997; Dual-axis tomography: an approach with alignment methods that preserve resolution. J Struct Biol 120:343–352 [CrossRef]
    [Google Scholar]
  9. Maszenan A. M., Seviour R. J., Patel B. K. C., Schumann P., Burghardt J., Tokiwa Y., Stratton H. M. 2000; Three isolates of novel polyphosphate-accumulating Gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp.nov. and Tetrasphaera australiensis sp. nov. Int J Syst Evol Microbiol 50:593–603 [CrossRef]
    [Google Scholar]
  10. McKenzie C. M., Seviour E. M., Schumann P., Maszenan A. M., Liu J. R., Webb R. I., Monis P., Saint C. P., Steiner U., Seviour R. J. 2006; Isolates of ‘ Candidatus Nostocoida limicola’ Blackall et al. 2000 should be described as three novel species of the genus Tetrasphaera , asTetrasphaera jenkinsii sp. nov., Tetrasphaera vanveenii sp. nov. and Tetrasphaera veronensis sp. nov. Int J Syst Evol Microbiol 56:2279–2290 [CrossRef]
    [Google Scholar]
  11. Moissl C., Hosoya N., Bruckner J., Stuecker T., Roman M., Venkateswaran K. 2007; Molecular microbial community structure of the Regenerative Enclosed Life Support Module Simulator (REMS) air system. Int J Astrobiol 6:131–145 [CrossRef]
    [Google Scholar]
  12. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology . pp 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  13. Saha P., Krishnamurthi S., Mayilraj S., Prasad G. S., Bora T. C., Chakrabarti T. 2005; Aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of Assam, India. Int J Syst Evol Microbiol 55:1491–1495 [CrossRef]
    [Google Scholar]
  14. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  15. Zheng Q. S., Braunfeld M. B., Sedat J. W., Agard D. A. 2004; An improved strategy for automated electron microscopic tomography. J Struct Biol 147:91–101 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65137-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65137-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error